Protein Alignment Atg4b and Atg4c

DIOPT Version :9

• Sequence 1: NP_650452.1
• Gene: Atg4b / 41868
• FlyBaseID: FBgn0038325
• Length: 668
• Species: Drosophila melanogaster
• Sequence 2: NP_001101418.1
• Gene: Atg4c / 313391
• RGDID: 1598323
• Length: 458
• Species: Rattus norvegicus
• Alignment Length: 578
• Identity: 189/578 - (32%)
• Similarity: 269/578 - (46%)
• Gaps: 186/578 - (32%)

  Fly   146 MESKLVAMWHNVKYGWSGKMRQTSFSKEQPVWLLGRCYHRRFTPPVSMESSITELPSGADTTPDN 210
            :::|.::.|:|:||.|..|.: |.||:..||.|||:|||.::..    ||.:....||.
  Rat    12 LKTKFISAWNNMKYSWVLKTK-TYFSRNSPVLLLGKCYHFKYED----ESKVLPARSGC------ 65

  Fly   211 ATSAFDSIQATSTSTSLYPALNPQQIDEIVVPQELGMDAVENQV--GEQPWEEGIEGFRRDFYSR 273
                                                  |:|:.|  |      .:|.||:||.||
  Rat    66 --------------------------------------AIEDHVIAG------NVEEFRKDFISR 86

  Fly   274 IWMTYRREFPIMNGSNYTSDCGWGCMLRSGQMLFAQGLICHFLGRSWRY---------DSESQL- 328
            ||:|||.|||.:..|..|:||||||.||:||||.|||||.|||||:|.:         ||:|..
  Rat    87 IWLTYREEFPQIEASALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALHIESSDSDSWTS 151

  Fly   329 -------------------------------------HSTYEDNMHKKIVKWFGDSSSKSSPFSI 356
                                                 |:...:..|::|:.||||  |..:.|.:
  Rat   152 NTIHKFTASFEASLSGERELRTPAVSLKETSGKHPDDHAVQSEIYHRQIISWFGD--SPVAVFGL 214

  Fly   357 HALVRLGEHLGKKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSI 421
            |.|:..|:..|||.|||||||.|:::|:.|:|.|  .:.|...:::|||:|||:|..|:.|:
  Rat   215 HRLIEFGKKSGKKAGDWYGPAVVAHILRKAVEEA--RHPDLQGLTIYVAQDCTVYNSDVIDK--- 274

  Fly   422 PEPAPKPHVPWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLG 486
                             |.::. |......|::|:|:|:|||.::.|..|...:|.:||.|:|:|
  Rat   275 -----------------QTDSV-TAGDARDKAVIILVPVRLGGERTNIDYLEFVKGVLSLEYCVG 321

  Fly   487 ILGGKPKHSLYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGF 551
            |:|||||.|.||.|||:|.||::||||||..|||:.::|.|.:|||.||:|:...||||||.|||
  Rat   322 IIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGF 386

  Fly   552 YCATKSDFDNFMESVQLYLHPMRCASGATVDKAAGSHHTTPQSSHQTEQTEMNYPLFSFSRGRCM 616
            ||....||:...|.:...|                           ...::..||||:|..|
  Rat   387 YCRNVQDFERASEEITKML---------------------------KISSKEKYPLFTFVNG--- 421

  Fly   617 DHERDEMSDSLYKPLIKQVASLAQELGAQLAPPQHGDEHDQDDSE------SEEFVLL 668
             |.||          ....::.|.|          .|...:|:.:      :||||||
  Rat   422 -HSRD----------FDFTSTAASE----------EDLFLEDEKKNFKRFSTEEFVLL 458

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Atg4b	NP_650452.1	Peptidase_C54	264..565	CDD:281417	142/347 (41%)
Atg4c	NP_001101418.1	Peptidase_C54	76..395	CDD:397471	141/343 (41%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166341343
Domainoid	1	1.000	302	1.000	Domainoid score	I1352
eggNOG	1	0.900	-	-		E1_KOG2674
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D332531at33208
OrthoFinder	1	1.000	-	-		FOG0000466
OrthoInspector	1	1.000	-	-		otm45424
orthoMCL	1	0.900	-	-		OOG6_100501
Panther	1	1.100	-	-	O	PTHR22624
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X289
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	13	12.710