Protein Alignment Atg4b and Atg4c

DIOPT Version :9

• Sequence 1: NP_650452.1
• Gene: Atg4b / 41868
• FlyBaseID: FBgn0038325
• Length: 668
• Species: Drosophila melanogaster
• Sequence 2: NP_001139439.1
• Gene: Atg4c / 242557
• MGIID: 2651854
• Length: 458
• Species: Mus musculus
• Alignment Length: 573
• Identity: 188/573 - (32%)
• Similarity: 268/573 - (46%)
• Gaps: 176/573 - (30%)

  Fly   146 MESKLVAMWHNVKYGWSGKMRQTSFSKEQPVWLLGRCYHRRFTPPVSMESSITELPSGADTTPDN 210
            :::|.::.|:|:||.|..|.: |.||:..||.|||:|||.::..    ||.:....||.
Mouse    12 LKTKFISAWNNMKYSWVLKTK-TYFSRNSPVLLLGKCYHFKYED----ESKMLPARSGC------ 65

  Fly   211 ATSAFDSIQATSTSTSLYPALNPQQIDEIVVPQELGMDAVENQV--GEQPWEEGIEGFRRDFYSR 273
                                                  |:|:.|  |      .:|.||:||.||
Mouse    66 --------------------------------------AIEDHVIAG------NVEEFRKDFISR 86

  Fly   274 IWMTYRREFPIMNGSNYTSDCGWGCMLRSGQMLFAQGLICHFLGRSWRY---------DSESQL- 328
            ||:|||.|||.:..|..|:||||||.||:||||.|||||.|||||:|.:         ||:|..
Mouse    87 IWLTYREEFPQIEASALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALHIENADSDSWTS 151

  Fly   329 -------------------------------------HSTYEDNMHKKIVKWFGDSSSKSSPFSI 356
                                                 |:...:..|:||:.||||  |..:.|.:
Mouse   152 NTVKKFTASFEASLSGDRELRTPAVSLKETSGKCPDDHAVRNEAYHRKIISWFGD--SPVAVFGL 214

  Fly   357 HALVRLGEHLGKKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSI 421
            |.|:..|:..|||.|||||||.|:::|:.|:|.|  .:.|...:::|||:|||:|..|:.|:
Mouse   215 HRLIEFGKKSGKKAGDWYGPAVVAHILRKAVEEA--RHPDLQGLTIYVAQDCTVYNSDVIDK--- 274

  Fly   422 PEPAPKPHVPWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLG 486
                             |.::. |......|::|:|:|:|||.::.|..|...:|.:||.|:|:|
Mouse   275 -----------------QTDSV-TAGDARDKAVIILVPVRLGGERTNTDYLEFVKGVLSLEYCVG 321

  Fly   487 ILGGKPKHSLYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGF 551
            |:|||||.|.||.|||:|.||::||||||..|||:.::|.|.:|||.||:|:...||||||.|||
Mouse   322 IIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGF 386

  Fly   552 YCATKSDFDNFMESVQLYLHPMRCASGATVDKAAGSHHTTPQSSHQTEQTEMNYPLFSFSRGRCM 616
            ||....||:...|.:...|                           ...::..||||:|..|...
Mouse   387 YCRNVQDFERASEEITKML---------------------------KISSKEKYPLFTFVNGHSK 424

  Fly   617 DHERDEMSDSLYKPLIKQVASLAQELGAQLAPPQHGDEHDQ-DDSESEEFVLL 668
            |.:            ....|:..::|.::       ||... ....:||||||
Mouse   425 DFD------------FTSTAASEEDLFSE-------DERKNFKRFSTEEFVLL 458

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Atg4b	NP_650452.1	Peptidase_C54	264..565	CDD:281417	143/347 (41%)
Atg4c	NP_001139439.1	Peptidase_C54	77..395	CDD:281417	142/342 (42%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837621
Domainoid	1	1.000	302	1.000	Domainoid score	I1399
eggNOG	1	0.900	-	-		E1_KOG2674
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	364	1.000	Inparanoid score	I2154
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D332531at33208
OrthoFinder	1	1.000	-	-		FOG0000466
OrthoInspector	1	1.000	-	-		otm43355
orthoMCL	1	0.900	-	-		OOG6_100501
Panther	1	1.100	-	-	O	PTHR22624
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R109
SonicParanoid	1	1.000	-	-		X289
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.790