Protein Alignment Atg4b and Atg4d

DIOPT Version :9

• Sequence 1: NP_650452.1
• Gene: Atg4b / 41868
• FlyBaseID: FBgn0038325
• Length: 668
• Species: Drosophila melanogaster
• Sequence 2: NP_705811.8
• Gene: Atg4d / 235040
• MGIID: 2444308
• Length: 474
• Species: Mus musculus
• Alignment Length: 628
• Identity: 204/628 - (32%)
• Similarity: 285/628 - (45%)
• Gaps: 214/628 - (34%)

  Fly    70 RVPSPSPVSTSGANLNLKTENAATATPQRKISTSSRFMNAFSQLYGGGNSGPSCGHVEQHNEEPG 134
            |:|.||.:..||:.:      ||                     .|...:.|:         ||.
Mouse    32 RIPDPSNLGPSGSGV------AA---------------------LGSSGTDPA---------EPD 60

  Fly   135 DLSANRTPTKGMESKLVAMWHNVKYGWSGKMRQTSFSKEQPVWLLGRCYHRRFTPPVSMESSITE 199
            ::..       .::|.:..|:||||||:.|.| |||||...|.|.|||||
Mouse    61 EVDK-------FKAKFLTAWNNVKYGWAVKSR-TSFSKISTVHLCGRCYH--------------- 102

  Fly   200 LPSGADTTPDNATSAFDSIQATSTSTSLYPALNPQQIDEIVVPQELGMDAVENQVGEQPWEEGIE 264
                           |:.                                          |..|:
Mouse   103 ---------------FEG------------------------------------------EGDIQ 110

  Fly   265 GFRRDFYSRIWMTYRREFPIMNGSNYTSDCGWGCMLRSGQMLFAQGLICHFLGRSWRYDSESQLH 329
            .|:|||.||:|:||||:||.:.|.:.|||||||||||||||:.||||:.|||.|.||:...:.|.
Mouse   111 RFQRDFVSRLWLTYRRDFPPLAGGSLTSDCGWGCMLRSGQMMLAQGLLLHFLPRDWRWVEGTGLA 175

  Fly   330 ST-----------------------------YEDNMHKKIVKWFGDSSSKSSPFSIHALVRLGEH 365
            |:                             .:|..|::||.||.|  ...:||.:|.||.||..
Mouse   176 SSEMPGPASPSRCRGPGRRGPPRWTQGALEMEQDRWHRRIVSWFAD--HPRAPFGLHRLVELGRS 238

  Fly   366 LGKKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQDIEDQCSIPEPAPKPHV 430
            .|||.||||||:.|:::|:.|:|..    ::...:.|||::|||:|..|:....|.|:|..:
Mouse   239 SGKKAGDWYGPSVVAHILRKAVESC----SEVSRLVVYVSQDCTVYKADVARLLSWPDPTAE--- 296

  Fly   431 PWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHCLKLLLSTEHCLGILGGKPKHS 495
                                |||:::|:|:|||.:.|||||..|:|.||.:|.||||:||||:||
Mouse   297 --------------------WKSVVILVPVRLGGETLNPVYVPCVKELLRSELCLGIMGGKPRHS 341

  Fly   496 LYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKSPRKLKASKMDPSCCIGFYCATKSDFD 560
            |||:|:|:|.|::|||||||..|||:|.:|.|.||||.||||:..:||||||.:|||...:.:|:
Mouse   342 LYFIGYQDDFLLYLDPHYCQPTVDVSQPSFPLESFHCTSPRKMAFAKMDPSCTVGFYAGNRKEFE 406

  Fly   561 NFMESVQLYLHPMRCASGATVDKAAGSHHTTPQSSHQTEQTEMNYPLFSFSRGRCMDHERDEMSD 625
            .....:      ||..|.::|                ||:    ||:|:.:.|...||..|.:..
Mouse   407 TLCSEL------MRILSSSSV----------------TER----YPMFTVAEGHAQDHSLDALCT 445

  Fly   626 SLYKPLIKQVASLAQELGAQLAPPQHGDEHDQDDSESEEFVLL 668
            .|.:|.::.              |..|.........||:||.|
Mouse   446 QLSQPTLRL--------------PCTGRLLKAKRPSSEDFVFL 474

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Atg4b	NP_650452.1	Peptidase_C54	264..565	CDD:281417	145/329 (44%)
Atg4d	NP_705811.8	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..60		11/63 (17%)
		Cryptic mitochondrial signal peptide. /evidence=ECO:0000250	64..103		22/76 (29%)
		Peptidase_C54	110..411	CDD:281417	145/329 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	175..198		1/22 (5%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837620
Domainoid	1	1.000	302	1.000	Domainoid score	I1399
eggNOG	1	0.900	-	-		E1_KOG2674
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H13156
Inparanoid	1	1.050	364	1.000	Inparanoid score	I2154
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D332531at33208
OrthoFinder	1	1.000	-	-		FOG0000466
OrthoInspector	1	1.000	-	-		otm43355
orthoMCL	1	0.900	-	-		OOG6_100501
Panther	1	1.100	-	-	LDO	PTHR22624
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R109
SonicParanoid	1	1.000	-	-		X289
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.790