DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsc70-4 and dnaK

DIOPT Version :9

Sequence 1:	NP_001262586.1	Gene:	Hsc70-4 / 41840	FlyBaseID:	FBgn0266599	Length:	651	Species:	Drosophila melanogaster
Sequence 2:	NP_414555.1	Gene:	dnaK / 944750	ECOCYCID:	EG10241	Length:	638	Species:	Escherichia coli

Alignment Length:	622	Identity:	300/622 - (48%)
Similarity:	402/622 - (64%)	Gaps:	40/622 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPTQTIFDA 70
            :|||||||.|||.:.......::.|.:|:|||||.:|:| |.|.|:|..||.|...||..|:|..
E. coli     5 IGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAI 69

  Fly    71 KRLIGRKFDDAAVQSDMKHWPFEVVSAD-GKPKIEVTYKDEKKTFFPEEISSMVLTKMKETAEAY 134
            ||||||:|.|..||.|:...||::::|| |...:||  |.:|..  |.:||:.||.|||:|||.|
E. coli    70 KRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEV--KGQKMA--PPQISAEVLKKMKKTAEDY 130

  Fly   135 LGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFD 199
            ||:.||.|||||||||||:|||||||||.||||.|.||||||||||:||||| |..|.|.:.::|
E. coli   131 LGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLD-KGTGNRTIAVYD 194

  Fly   200 LGGGTFDVSILSID--DG--IFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRAL 260
            |||||||:||:.||  ||  .|||.:|.|||||||||||:||:.:.|:|||:....||..:..|:
E. coli   195 LGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAM 259

  Fly   261 RRLRTACERAKRTLSSSTQASIEIDSLFEGT----DFYTSITRARFEELNADLFRSTMDPVEKAL 321
            :||:.|.|:||..|||:.|..:.:..:....    .....:|||:.|.|..||...:::|::.||
E. coli   260 QRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVAL 324

  Fly   322 RDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQ 386
            :||.|..|.|.|::||||.||:|.||:.:.:.| |||..|.:|||||||.|||||..:|.||
E. coli   325 QDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD--- 385

  Fly   387 EVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTK 451
             |:|:||||||||||||||.||||:.||.:|||||||.:|.|:|..|||..|.|.|.:|||....
E. coli   386 -VKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAA 449

  Fly   452 DNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIE 516
            ||..||:|.|.||.|||||:|||||||||||:|||:|:|.::::.||.||||....| |::::|:
E. coli   450 DNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSG-LNEDEIQ 513

  Fly   517 RMVNEAEKYRNEDEKQKETIAAKNGLESYCFNMKATLDEDN-----------------LKTKISD 564
            :||.:||.....|.|.:|.:..:|..:....:.:..::|..                 |:|.:..
E. coli   514 KMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKG 578

  Fly   565 SDRTTILDKCNETIKWLDANQLADKEEYEHRQKELEG 601
            .|:..|..|..|..:  .:.:|.:..:.:|.|::..|
E. coli   579 EDKAAIEAKMQELAQ--VSQKLMEIAQQQHAQQQTAG 613

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsc70-4	NP_001262586.1	PTZ00009	1..612	CDD:240227	300/622 (48%)
Hsc70-4	NP_001262586.1	HSPA1-2_6-8-like_NBD	6..381	CDD:212675	199/383 (52%)
dnaK	NP_414555.1	dnaK	1..638	CDD:234715	300/622 (48%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
	3	2.810