DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsc70-4 and HSPA5

DIOPT Version :9

Sequence 1:	NP_001262586.1	Gene:	Hsc70-4 / 41840	FlyBaseID:	FBgn0266599	Length:	651	Species:	Drosophila melanogaster
Sequence 2:	NP_005338.1	Gene:	HSPA5 / 3309	HGNCID:	5238	Length:	654	Species:	Homo sapiens

Alignment Length:	617	Identity:	401/617 - (64%)
Similarity:	500/617 - (81%)	Gaps:	6/617 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPTQTIFDA 70
            |||||||||||||||::|:|||||||||||.|||||||| :.|||||||||||:..||..|:|||
Human    31 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 95

  Fly    71 KRLIGRKFDDAAVQSDMKHWPFEVVSADGKPKIEVTY-KDEKKTFFPEEISSMVLTKMKETAEAY 134
            ||||||.::|.:||.|:|..||:||....||.|:|.. ..:.|||.|||||:|||||||||||||
Human    96 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 160

  Fly   135 LGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFD 199
            |||.||:||:||||||||:||||||||||||||||:|||||||||||||||||:. ||:|:|:||
Human   161 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKRE-GEKNILVFD 224

  Fly   200 LGGGTFDVSILSIDDGIFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRALRRLR 264
            |||||||||:|:||:|:|||.:|.|||||||||||.|::.||::.:|:|..||:..:.||:::||
Human   225 LGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLR 289

  Fly   265 TACERAKRTLSSSTQASIEIDSLFEGTDFYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKS 329
            ...|:|||.|||..||.|||:|.:||.||..::|||:|||||.|||||||.||:|.|.|:.|.||
Human   290 REVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKS 354

  Fly   330 VIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLL 394
            .|.:||||||||||||:|:|:::.|||||.::.|||||||||||||||.:|.||  |:..||:||
Human   355 DIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLL 417

  Fly   395 DVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKF 459
            ||.||:|||||.||||:.||.|||.:|||::|.|:|.|||||.|.|:||||||.:||||:|||.|
Human   418 DVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTF 482

  Fly   460 ELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEK 524
            :|:||||||||||||||||:||.||||.|||.::.|..:||||||||:.||:.|:||||||:|||
Human   483 DLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEK 547

  Fly   525 YRNEDEKQKETIAAKNGLESYCFNMKATL-DEDNLKTKISDSDRTTILDKCNETIKWLDANQLAD 588
            :..||:|.||.|..:|.||||.:::|..: |::.|..|:|..|:.|:.....|.|:||:::|.||
Human   548 FAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDAD 612

  Fly   589 KEEYEHRQKELEGVCNPIITKLYQGAGFPPGG 620
            .|:::.::||||.:..|||:|||..||.||.|
Human   613 IEDFKAKKKELEEIVQPIISKLYGSAGPPPTG 644

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsc70-4	NP_001262586.1	PTZ00009	1..612	CDD:240227	395/607 (65%)
Hsc70-4	NP_001262586.1	HSPA1-2_6-8-like_NBD	6..381	CDD:212675	260/375 (69%)
HSPA5	NP_005338.1	Required for interaction with KIAA1324. /evidence=ECO:0000269\|PubMed:26045166	1..80		41/48 (85%)
		HSP70	30..635	CDD:394970	394/606 (65%)
		Nucleotide-binding (NBD). /evidence=ECO:0000303\|PubMed:28286085	125..280		114/155 (74%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	409..419		5/9 (56%)
		Substrate-binding (SBD). /evidence=ECO:0000303\|PubMed:28286085	420..500		57/79 (72%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	633..654		8/12 (67%)
		Prevents secretion from ER. /evidence=ECO:0000255	651..654
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	1	1.010	-	-		D288077at2759
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	7	6.730