DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsc70-4 and Hspa5

DIOPT Version :9

Sequence 1:	NP_001262586.1	Gene:	Hsc70-4 / 41840	FlyBaseID:	FBgn0266599	Length:	651	Species:	Drosophila melanogaster
Sequence 2:	NP_037215.1	Gene:	Hspa5 / 25617	RGDID:	2843	Length:	654	Species:	Rattus norvegicus

Alignment Length:	617	Identity:	401/617 - (64%)
Similarity:	499/617 - (80%)	Gaps:	6/617 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAMNPTQTIFDA 70
            |||||||||||||||::|:|||||||||||.|||||||| :.|||||||||||:..||..|:|||
  Rat    31 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 95

  Fly    71 KRLIGRKFDDAAVQSDMKHWPFEVVSADGKPKIEVTY-KDEKKTFFPEEISSMVLTKMKETAEAY 134
            ||||||.::|.:||.|:|..||:||....||.|:|.. ..:.|||.|||||:|||||||||||||
  Rat    96 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 160

  Fly   135 LGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFD 199
            |||.||:||:||||||||:||||||||||||||||:|||||||||||||||||:. ||:|:|:||
  Rat   161 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKRE-GEKNILVFD 224

  Fly   200 LGGGTFDVSILSIDDGIFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRALRRLR 264
            |||||||||:|:||:|:|||.:|.|||||||||||.|::.||::.:|:|..||:..:.||:::||
  Rat   225 LGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLR 289

  Fly   265 TACERAKRTLSSSTQASIEIDSLFEGTDFYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKS 329
            ...|:|||.|||..||.|||:|.|||.||..::|||:|||||.|||||||.||:|.|.|:.|.||
  Rat   290 REVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKS 354

  Fly   330 VIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLL 394
            .|.:||||||||||||:|:|:::.|||||.::.|||||||||||||||.:|.||  |:..||:||
  Rat   355 DIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGD--QDTGDLVLL 417

  Fly   395 DVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKF 459
            ||.||:|||||.||||:.||.|||.:|||::|.|:|.|||||.|.|:||||||.:||||:|||.|
  Rat   418 DVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTF 482

  Fly   460 ELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEK 524
            :|:||||||||||||||||:||.||||.|||.::.|..:||||||||:.||:.|:||||||:|||
  Rat   483 DLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEK 547

  Fly   525 YRNEDEKQKETIAAKNGLESYCFNMKATL-DEDNLKTKISDSDRTTILDKCNETIKWLDANQLAD 588
            :..||:|.||.|..:|.||||.:::|..: |::.|..|:|..|:.|:.....|.|:||:::|.||
  Rat   548 FAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSPEDKETMEKAVEEKIEWLESHQDAD 612

  Fly   589 KEEYEHRQKELEGVCNPIITKLYQGAGFPPGG 620
            .|:::.::||||.:..|||:|||...|.||.|
  Rat   613 IEDFKAKKKELEEIVQPIISKLYGSGGPPPTG 644

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsc70-4	NP_001262586.1	PTZ00009	1..612	CDD:240227	396/607 (65%)
Hsc70-4	NP_001262586.1	HSPA1-2_6-8-like_NBD	6..381	CDD:212675	261/375 (70%)
Hspa5	NP_037215.1	Required for interaction with KIAA1324. /evidence=ECO:0000250\|UniProtKB:P11021	1..80		41/48 (85%)
		HSPA5-like_NBD	28..403	CDD:212681	260/372 (70%)
		HSP70	30..635	CDD:278441	395/606 (65%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250\|UniProtKB:P11021	125..280		114/155 (74%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	409..419		5/9 (56%)
		Substrate-binding (SBD). /evidence=ECO:0000250\|UniProtKB:P11021	420..500		57/79 (72%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	631..654		8/14 (57%)
		Prevents secretion from ER	651..654
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	1	1.010	-	-		D288077at2759
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	6.730