Protein Alignment trx and Setd1a

DIOPT Version :10

• Sequence 1: NP_476769.1
• Gene: trx / 41737
• FlyBaseID: FBgn0003862
• Length: 3726
• Species: Drosophila melanogaster
• Sequence 2: NP_821172.2
• Gene: Setd1a / 233904
• MGIID: 2446244
• Length: 1716
• Species: Mus musculus
• Alignment Length: 2148
• Identity: 433/2148 - (20%)
• Similarity: 691/2148 - (32%)
• Gaps: 730/2148 - (33%)

  Fly  1847 ALKANGSPSVTYES-NFEVSRPVYVELDRKRKKLIEPARVQFHIGSLEVRQLGAIVPRF--SDSY 1908
            ||:........|:. :|.||...|..::    .|.:|   :.|:.| :.|.....||:|  .:.|
Mouse    31 ALRRPSQKVYRYDGVHFSVSDSKYTPVE----DLQDP---RCHVRS-KARDFSLPVPKFKLDEFY 87

  Fly  1909 EAVVPINFLCSRLYWSSKEPWKIVEYTVRTTIQNSSSTLTALDVGRNYTVDHTNPNSKEVQLGMA 1973
            ...:|:.                 |.|......|...|... |:.|.|      ...:||::.:.
Mouse    88 IGQIPLK-----------------EVTFARLNDNVRETFLK-DMCRKY------GEVEEVEILLH 128

  Fly  1974 QIARWHTSLARSEFLENGGTDWSGEFPNPNSCVPPDENTEEEPQQQADLLPPELKDAIFEDLPHE 2038
            ...|.|..|||..|....|.                    :|..:...|      .::..::.|.
Mouse   129 PRTRKHLGLARVLFTSTRGA--------------------KETVKNLHL------TSVMGNIIHA 167

  Fly  2039 LLDGISMLDIFLYDDKTDLFAISEQSKDGTQAMTSNQAQNQNQQAGGANSVSICDEDTRNSNTSL 2103
            .||......:..|:      .|...|.......|..:|.::..|..||.:.:.......:|:|:.
Mouse   168 QLDIKGQQRMKYYE------LIVNGSYTPQTVPTGGKALSEKFQGSGAAAETTEARRRSSSDTAA 226

  Fly  2104 -----------GNGWPASNPVEDAMLSAARNSSQVQMLKTLAWPKLDGNSAMATAIKRR---KLS 2154
                       |||.|.|   :|...|::|..:      ..::.:....|:..|....|   ..|
Mouse   227 YPAGTTVGGTPGNGTPCS---QDTNFSSSRQDT------PSSFGQFTPQSSQGTPYTSRGSTPYS 282

  Fly  2155 KNLAEGVFLTLSSQQRNKKEMATVAGVSRRQ---------SISETSVEGVATTSGSVRSKSFTWS 2210
            ::.|.....|.:|.:..:.|.:.....|||.         :.:.||....||.:.|..|.|.:.|
Mouse   283 QDSAYSSSTTSTSFKPRRSENSYQDSFSRRHFSTSSAPATTATATSATAAATAASSSSSSSSSSS 347

  Fly  2211 AAKRYFEKSEGREEAAKMRIMQMDGVDDSITEFRIISGDGNLSTAQFSGQVKCDRCQCTYRNYDA 2275
            ::      |.....|::.|     |.|.|...:                          |.:::.
Mouse   348 SS------SSSSSSASQFR-----GSDSSYPAY--------------------------YESWNR 375

  Fly  2276 FQRHLPSCSPTMSSNETESDVSGQGMTNNATQISAESLNELQKQLLA----NAGGLNYLQSATSF 2336
            :||| .|..|..::.|   |.||.....|    :||.........||    .:...:|...|:..
Mouse   376 YQRH-TSYPPRRATRE---DPSGASFAEN----TAERFPPSYTSYLAPEPNRSTDQDYRPPASEA 432

  Fly  2337 PQVQSLGSLGQFGLQGLQQLQLQPQSLGSGFFLSQPNPATQANTDDLQIYANSLQSLAANLGGGF 2401
            |..:.....|..|..|           |.|                           ....|||.
Mouse   433 PPPEPPEPGGGGGGSG-----------GGG---------------------------GGGGGGGG 459

  Fly  2402 TLAQP------TVTAPAQPQLIAVSTNPDGTQQFIQIPQTMQATTTPTATYQTLQATNTDKKIML 2460
            ....|      |...||.|   |.|.:|        .|:|.. .:.|.|.:.:|     |.:|.:
Mouse   460 GAPSPEREEARTPPRPASP---ARSGSP--------APETTN-ESVPFAQHSSL-----DSRIEM 507

  Fly  2461 PLTAAGKPLKTVATKAAQQAAVKQRQLKSGHQVKPIQAKL-----------QPHPQQHQQQQQTQ 2514
            .|    |..::..:..|.....::....:|...:...|::           .|.|...:....|.
Mouse   508 LL----KEQRSKFSFLASDTEEEEENSSAGPGARDAGAEVPSGAGHGPCTPPPAPANFEDVAPTG 568

  Fly  2515 VQQPITVMGQNLLQPQLLFQSSTQTQAPQIILPQAQPQNIISFVTGDGSQGQPLQYISIPTAGEY 2579
            ..:|    |.....|    :::.|.||.    |.:..:::  .::.|...|.|....:.|.....
Mouse   569 SGEP----GAARESP----KANGQNQAS----PCSSGEDM--EISDDDRGGSPPPAPTPPQQPPP 619

  Fly  2580 KPQPQPTATPTFLTTAPGAG-----ATYLQT-----------------------DASGNLVLTTT 2616
            .|.|.|...|.:|.:.| .|     ..||..                       |...:|.|
Mouse   620 PPPPPPPPPPPYLASLP-LGYPPHQPAYLLPPRPDGPPPPEYPPPPPPPPPHIYDFVNSLEL--- 680

  Fly  2617 PSNSGLQMLTAQ------SLQAQPQVIGTLIQPQTIQLGGGADGNQPGSNQQPLILGGTGGGSSG 2675
                 :..|.||      |.|.|.|::..|.|   ::.|.|.....          .|..||:.|
Mouse   681 -----MDRLGAQWGGMPMSFQMQTQMLTRLHQ---LRQGKGLTAAS----------AGPPGGAFG 727

  Fly  2676 LEFATTSPQVILATQPMYYGL-----------------ETIVQNTVMSSQQFVSTAMPGMLSQNA 2723
            ..|....|     .|...|||                 |.......|:::...|:::.|   :.|
Mouse   728 EAFLPFPP-----PQEAAYGLPYALYTQGQEGRGSYSREAYHLPLPMAAEPLPSSSVSG---EEA 784

  Fly  2724 SFSATTTQVFQASKIEPIVDLPAGYVVLNNTGDASSAGTFLNAASVLQQQ----TQDDTTTQILQ 2784
            ............||:.|                  ||||.....:.|.|:    .|.|...::::
Mouse   785 RLPHREEAEIAESKVLP------------------SAGTVGRVLATLVQEMKSIMQRDLNRKMVE 831

  Fly  2785 NANF----------QFQSVPTSSGA--------STSMDYTSPVMVT----AKIPPVTQIKRTNAQ 2827
            |..|          :.::.|..:.|        ...|....|.|::    ||...:|.|:   |.
Mouse   832 NVAFGAFDQWWESKEEKAKPFQNAAKQQAKEEDKEKMKLKEPGMLSLVDWAKSGGITGIE---AF 893

  Fly  2828 AKAAGISGVGKVPPQPQVVNKVLPTSIVTQQSQVQVKNSNLKQSQVKGKAASGTGTTCGAPPSIA 2892
            |..:|:.|..::|                             ..:||.|           .||..
Mouse   894 AFGSGLRGALRLP-----------------------------SFKVKRK-----------EPSEI 918

  Fly  2893 SKPLQKKTNMIRPIHKLEVKPKVMKPTPKVQNQNHSLLQQQQQQQPQLQQQI--PAVVVNQVPKV 2955
            |:..::|                 :|.|       |...::.:..|:.:::.  |.....:.||
Mouse   919 SEASEEK-----------------RPRP-------STPAEEDEDDPEREKEAGEPGRPGTKPPK- 958

  Fly  2956 TISQQRIPAQ-------TQQQQLQQAQMIHIPQQQQPLQQQQVQVQPSMPIITLAEAPVVQSQFV 3013
             ..::|...|       |...:.::|......::.:....:..:.:
Mouse   959 -RDEERGKTQGKHRKSFTLDSEGEEASQESSSEKDEDDDDEDEEDE------------------- 1003

  Fly  3014 MEPQALEQQELANRVQHFSTSS------SSSSSNCSL---------PTNVVNPMQQQAPSTTSSS 3063
                  ||:|..:..:..:.:|      |.|||.|||         .|:........:.|::|||
Mouse  1004 ------EQEEAVDATKKEAEASDGEDEDSDSSSQCSLYADSDGENGSTSDSESGSSSSSSSSSSS 1062

  Fly  3064 TTRPTNRVLPMQQRQEPAPLSNECPVVSSPTP-PKPVEQPIIHQMTSASVSKCYAQKSTLPS-PV 3126
            ::..::.....::.::.|.:.:..|....|.| |.|.|:|....:..:.|.. .::|.|||: |.
Mouse  1063 SSSSSSSESSSEEEEQSAVIPSASPPREVPEPLPAPDEKPETDGLVDSPVMP-LSEKETLPTQPA 1126

  Fly  3127 YEAELKVSSVLE---------------------SIVP----------DVTMDAILEEQPVTESIY 3160
            ..||....||.:                     |.:|          .|:..| .||.||.|...
Mouse  1127 GPAEEPPPSVPQPPAEPPAGPPDAAPRLDERPSSPIPLLPPPKKRRKTVSFSA-AEEAPVPEPST 1190

  Fly  3161 TEGLYEKNS-PGESKTEQLLLQQQQREQLNQQLVNNGYLLDKHTFQVEPMDTDVYR--------- 3215
            ...|..|:| |...|..:::          ::.:.| ..|| |...|:....:|.|
Mouse  1191 AAPLQAKSSGPVSRKVPRVV----------ERTIRN-LPLD-HASLVKSWPEEVARGGRNRAGGR 1243

  Fly  3216 -----EEDLEEEEDEDD-----DFSLK-----MATSACNDHEMSDSEEPAVKDKI---SKILDN- 3261
                 ||:..|...|.|     |.:|.     :||....|    |||.....|:.   |.:|.:
Mouse  1244 VRSTEEEEATESGTEVDLAVLADLALTPARRGLATLPTGD----DSEATETSDEAERPSPLLSHI 1304

  Fly  3262 LTNDDCADSIATATTMEVDASAGYQQMVEDVLATTAAQSAPTEEFEGALETAAVEAAATYINEMA 3326
            |...:.|.:|....|...      .:.:|...|..|..|:|.:|...|.|....|         |
Mouse  1305 LLEHNYALAIKPPPTTPA------PRPLEPAPALAALFSSPADEVLEAPEVVVAE---------A 1354

  Fly  3327 DAHVLDLKQLQNGVELELRRRKEEQRTVSQEQEQSKAAIV-----------------PTAAAPEP 3374
            :.....|:|.....|.|.....||:.:.|.|...|.::..                 |....|.|
Mouse  1355 EEPKQQLQQQHPEQEGEEEEEDEEEESESSESSSSSSSDEEGAIRRRSLRSHTRRRRPPLPPPPP 1419

  Fly  3375 PQPIQEPK----KMTGPHLLYEIQSEDGFTYKSSSITEIWEKVFEAVQVARRAHGLTPLPEGPLA 3435
            |.|..||:    :||   :||                :||..            ||      .|.
Mouse  1420 PPPSFEPRSEFEQMT---ILY----------------DIWNS------------GL------DLE 1447

  Fly  3436 DMGGIQMIGLKTNALKYLIEQLPGVEKCSK-----------YTPKYHKRNGNVSTAANGAHGGNL 3489
            ||..:::      ..:.|::|..|.:..:.           .|||..:|      ..:|......
Mouse  1448 DMSYLRL------TYERLLQQTSGADWLNDTHWVQHTITNLSTPKRKRR------PQDGPREHQT 1500

  Fly  3490 GGSSAS-------------------AALSVSGGDSHGLLDYGSDQDELEENAYDCARCEPYSNRS 3535
            |.:.:.                   :|..:.|||:.|                        :||.
Mouse  1501 GSARSEGYYPISKKEKDKYLDVCPVSARQLEGGDTQG------------------------TNRV 1541

  Fly  3536 EYDMFSWLASRHRKQPIQVFVQPSDNELVPRRGTGSNLPM------AMKYRTLKETYKDYVGVFR 3594
                   |:.|..:|          ..|:...||.:.:..      .:|:|..|..:.      |
Mouse  1542 -------LSERRSEQ----------RRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFG------R 1583

  Fly  3595 SHIHGRGLYCTKDIEAGEMVIEYAGELIRSTLTDKRERYYDSRGIG-CYMFKIDDNLVVDATMRG 3658
            |.||..||:..:.|.|.||||||.|:.||..:.|.||:.|...||| .|:|::|.:.::|||..|
Mouse  1584 SRIHEWGLFAMEPIAADEMVIEYVGQNIRQMVADMREKRYVQEGIGSSYLFRVDHDTIIDATKCG 1648

  Fly  3659 NAARFINHCCEPNCYSKVVDILGHKHIIIFALRRIVQGEELTYDYKFPFEDEKIPCSCGSKRCRK 3723
            |.||||||||.||||:||:.|...|.|:|::.:.|...||:|||||||.||.||||.||::.||.
Mouse  1649 NLARFINHCCTPNCYAKVITIESQKKIVIYSKQPIGVDEEITYDYKFPLEDNKIPCLCGTESCRG 1713

  Fly  3724 YLN 3726
            .||
Mouse  1714 SLN 1716

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trx	NP_476769.1	NR_DBD_like	762..>856	CDD:413390
		PRK13914	<1006..>1224	CDD:237555
		PHD1_KMT2A_like	1268..1344	CDD:276981
		PHD	1346..1390	CDD:214584
		PHD3_KMT2A_like	1423..1479	CDD:276983
		ePHD_KMT2A_like	1737..1841	CDD:277134
		FYRN	1890..1937	CDD:461787	9/48 (19%)
		FYRC	3388..3476	CDD:197781	16/98 (16%)
		SET_KMT2A_2B	3575..3726	CDD:380947	73/157 (46%)
Setd1a	NP_821172.2	Interaction with WDR82. /evidence=ECO:0000250|UniProtKB:O15047	60..89		9/32 (28%)
		RRM_Set1A	92..186	CDD:409964	24/149 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	194..367		40/192 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	380..499		34/175 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	516..670		26/168 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	849..869		2/19 (11%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	911..1206		64/358 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1230..1259		5/28 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1275..1297		7/25 (28%)
		HCFC1-binding motif (HBM). /evidence=ECO:0000250|UniProtKB:O15047	1307..1311		0/3 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1355..1427		15/71 (21%)
		Interaction with CFP1. /evidence=ECO:0000250|UniProtKB:O15047	1424..1459		13/77 (17%)
		N-SET	1434..1571	CDD:463344	32/226 (14%)
		Interaction with ASH2L, RBBP5 and WDR5. /evidence=ECO:0000250|UniProtKB:O15047	1459..1546		17/123 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1480..1508		6/33 (18%)
		WDR5 interaction motif (WIN). /evidence=ECO:0000250|UniProtKB:O15047	1501..1506		1/4 (25%)
		RxxxRR motif. /evidence=ECO:0000250|UniProtKB:P38827	1546..1551		1/14 (7%)
		SET_SETD1	1565..1712	CDD:380946	71/152 (47%)