Protein Alignment Polr2B and RPB2

DIOPT Version :10

• Sequence 1: NP_476706.1
• Gene: Polr2B / 41721
• FlyBaseID: FBgn0262955
• Length: 1176
• Species: Drosophila melanogaster
• Sequence 2: NP_014794.3
• Gene: RPB2 / 854322
• SGDID: S000005677
• Length: 1224
• Species: Saccharomyces cerevisiae
• Alignment Length: 1221
• Identity: 708/1221 - (57%)
• Similarity: 894/1221 - (73%)
• Gaps: 62/1221 - (5%)

  Fly     5 NEEELYEEE--NAEEISHELWQEACWIVINAYFDEKGLVRQQLDSFDEFIQMSVQRIV-EDSPAI 66
            |.|:.|:|:  ..|:.|..:..|..|.||:|:|.|||||.||||||::|:..::|.|: |||..|
Yeast     6 NSEKYYDEDPYGFEDESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTLI 70

  Fly    67 ELQAEAQHTSGEVETPPRFSLKFEQIYLSKPTHWEKDGSPSPMMPNEARLRNLTYSAPLYVDITK 131
             |:..||||:.......::.:.|.:||::||...|.||....:.|.||||||||||:.|:||: |
Yeast    71 -LEQLAQHTTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARLRNLTYSSGLFVDV-K 133

  Fly   132 TKNVEGLD---------------PVETQHQKTFIGKIPIMLRSTYCLLSQLTDRDLTELNECPLD 181
            .:..|.:|               ..:::..|.|||::||||||..|.||:.|:.||.:|.|||.|
Yeast   134 KRTYEAIDVPGRELKYELIAEESEDDSESGKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFD 198

  Fly   182 PGGYFIINGSEKVLIAQEKMATNTVYVF-SMKDGKYAFKTEIRSCLEHSSRPTSTLWVNMMARGS 245
            .|||||||||||||||||:.|.|.|.|| .......:...||||.||..||..|||.|.:..|
Yeast   199 MGGYFIINGSEKVLIAQERSAGNIVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGR-- 261

  Fly   246 QNIKKSAIGQRIIAILPYIKQEIPIMIVFRALGFVADRDILEHIIYDFDDPEMMEMVKPSLDEAF 310
                :.:..:.|.|.||||||:|||:|:|||||.:.|.:|||||.||.:|.:|:||:||.:::.|
Yeast   262 ----EGSSARTIKATLPYIKQDIPIVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGF 322

  Fly   311 VVQEQNVALNFIGARGARPGVTKDKRIKYAKEILQKEMLPHVGVSDFCETKKAYFLGYMVHRLLL 375
            |:|::..||:|||.||...|:.|:|||:|||:|||||.|||:...:..|::||:|||||::||||
Yeast   323 VIQDRETALDFIGRRGTALGIKKEKRIQYAKDILQKEFLPHITQLEGFESRKAFFLGYMINRLLL 387

  Fly   376 ASLGRRELDDRDHYGNKRLDLAGPLLAFLFRGLFKNLMKEVRMYTQKFIDRGKDFNLELAIKTNI 440
            .:|.|::.|||||:|.|||||||||||.||:.|||.|.|::..|.|:.::...|||::|||....
Yeast   388 CALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQRTVEEAHDFNMKLAINAKT 452

  Fly   441 ITDGLRYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRVNSPIGRDGKLAKPRQLHNT 505
            ||.||:|:|||||||:||||..:||||||||||.|::||||||||.|:|||||||||||||||||
Yeast   453 ITSGLKYALATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNT 517

  Fly   506 LWGMLCPAETPEGAAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEIAPSAIADATKIFV 570
            .||::||||||||.|.||||||:||:.||||:.|.||:.||.||.||.||:..|....|||::||
Yeast   518 HWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPLEDYVPHQSPDATRVFV 582

  Fly   571 NGCWVGIHRDPEQLMATLRKLRRQMDIIVSEVSMIRDIRDREIRIYTDAGRICRPLLIVENG--- 632
            ||.|.|:||:|.:||.|||.|||:.| |..|||||||||::|::|:|||||:.|||.|||:.
Yeast   583 NGVWHGVHRNPARLMETLRTLRRKGD-INPEVSMIRDIREKELKIFTDAGRVYRPLFIVEDDESL 646

  Fly   633 ---SLLLKKTHVEMLKERDYNN----------YSWQVLVASGVVEYIDTLEEETVMIAMSPYDLK 684
               .|.::|.|:..|...:|.:          |:|..|:..|:|||||..|||:::|||.|.||:
Yeast   647 GHKELKVRKGHIAKLMATEYQDIEGGFEDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLE 711

  Fly   685 Q---------DKDYA-------YCTTYTHCEIHPAMILGVCASIIPFPDHNQSPRNTYQSAMGKQ 733
            .         |.|.|       :.||:|||||||:|||||.||||||||||||||||||||||||
Yeast   712 PAEANEENDLDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQ 776

  Fly   734 AMGVYITNFHVRMDTLAHVLYYPMKPLVTTRSMEYLRFRELPAGINSIVAILCYTGYNQEDSVIL 798
            ||||::||::|||||:|::||||.|||.|||:||||:|||||||.|:||||.||:|||||||:|:
Yeast   777 AMGVFLTNYNVRMDTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIM 841

  Fly   799 NASAVERGFFRSVFYRSYKDSENKRVGDQEENFEKPHRGTCQGMRNAHYDKLDDDGIIAPGIRVS 863
            |.|:::||.|||:|:|||.|.|.|......|.||||.|.....|::..||||||||:||||:|||
Yeast   842 NQSSIDRGLFRSLFFRSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVS 906

  Fly   864 GDDVVIGKTITLPENDDELDSNTKRFSKRDASTFLRNSETGIVDQVMLTLNSEGYKFCKIRVRSV 928
            |:||:||||..:..:::||...|...|||||||.||::|.||||||::|.|.:|.||.|:|||:.
Yeast   907 GEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTTNQDGLKFVKVRVRTT 971

  Fly   929 RIPQIGDKFASRHGQKGTCGIQYRQEDMAFTCEGLAPDIIINPHAIPSRMTIGHLIECLQGKLGS 993
            :|||||||||||||||||.||.||:|||.||.||:.||:||||||||||||:.||||||..|:.:
Yeast   972 KIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVAA 1036

  Fly   994 NKGEIGDATPFNDAVNVQKISTFLQEYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVD 1058
            ..|..|||:||.| :.|:.||..|:|:||..||.|||||||||:|:.||:|.||||||||:||||
Yeast  1037 LSGNEGDASPFTD-ITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVD 1100

  Fly  1059 DKIHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQISHGAAQFLRERLFEVSDPYRVHICNF 1123
            ||||:|||||:|:|.|||:|||:||||||||||||||.|:||||.||:|||.|.||.:|||||..
Yeast  1101 DKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLMEASDAFRVHICGI 1165

  Fly  1124 CGLI-AIANLRNNTFECKGCKNKTQISQVRLPYAAKLLFQELMSMNIAPRL 1173
            |||: .||.|.:|.||||||.||..|.|:.:||||||||||||:|||.|||
Yeast  1166 CGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRL 1216

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Polr2B	NP_476706.1	Ricin_B_lectin	28..1176	CDD:481363	701/1196 (59%)
RPB2	NP_014794.3	Ricin_B_lectin	31..1216	CDD:481363	699/1194 (59%)