DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nsf2 and NSF

DIOPT Version :9

Sequence 1:	NP_001287318.1	Gene:	Nsf2 / 41694	FlyBaseID:	FBgn0266464	Length:	752	Species:	Drosophila melanogaster
Sequence 2:	NP_192400.2	Gene:	NSF / 825830	AraportID:	AT4G04910	Length:	742	Species:	Arabidopsis thaliana

Alignment Length:	731	Identity:	315/731 - (43%)
Similarity:	459/731 - (62%)	Gaps:	45/731 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 MRAIKCPTDELSLTNKAIVNVSDFTEEVKYVDIS-PGPGLHYI-----FALEKISGPELPLGHVG 67
            |.....|:.:|:.||.|..:.||..:      .| ||..|...     |.|.......:..|::.
plant    12 MTVTNTPSADLAFTNLAYCSSSDLRQ------FSVPGSDLFLANVADSFILSLCGHGSIRDGNIA 70

  Fly    68 FSLVQRKWATLSINQEIDVR----PYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFL 128
            .:.:||:.|.:|....:.|.    |..||     :.:::.|.:|: ||.|..|..|:..::.:..
plant    71 LNAIQRRHARVSTGDMVSVSRFVPPENFD-----LAMLTLELEFV-KKGTKSEQVDAALLSTQLK 129

  Fly   129 MQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKA 193
            .::....|||||...|::....:    :.|:...|.    :....|..:..|.:..:|.:.||.:
plant   130 RKYTNQVLTVGQKATFEYHGTNY----ILTVNRADV----EGQDHTNGIERGLLSKDTYIVFEAS 186

  Fly   194 ENS---VLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGI 255
            ..|   ::|.:..:...|.:    :.:::...:|||||..||..|||||||||||||.:..:|||
plant   187 NASGIKIVNQREAASSNIFK----HKEFNLESLGIGGLGAEFADIFRRAFASRVFPPHVTSRLGI 247

  Fly   256 KHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKR 320
            |||||:||:|||||||||||||||.|||.::|||||||::|.|:|||:|.|:|.|||:||::::.
plant   248 KHVKGMLLFGPPGTGKTLMARQIGKMLNGKDPKIVNGPEVLSKFVGETEKNVRDLFADAEQDQRT 312

  Fly   321 LGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMID 385
            ||..|.||:|||||||||||:|||....:||||::|||||.||||||.|||:|:||||||:|::|
plant   313 LGDASELHVIIFDEIDAICKSRGSTRDGTGVHDSIVNQLLTKIDGVEALNNVLLIGMTNRKDLLD 377

  Fly   386 EALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLV 450
            ||||||||||||:|||||:|.||:|||.|||.:|::.:.:.:|::..|:||:|||:|||||||:|
plant   378 EALLRPGRLEVQVEISLPDEAGRLQILQIHTNKMKENSFLGTDINLQELAARTKNYSGAELEGVV 442

  Fly   451 RAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINW 515
            ::|.|.|:||.:..|..  ..|...|.:::|..|||||: .:::|||||:.:.||.....|:::.
plant   443 KSATSYALNRQLSMDDL--TKPVEEENIKITMEDFLHAI-YEVQPAFGASTDDLERCRLNGMVDC 504

  Fly   516 GPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVG 580
            |.....:.:..||.|:|.|.:..|.||:.|:||...|||:||||.:...||||:||:.|.|.|:|
plant   505 GHRHNHIYKRAMLLVEQVKVSTRSPLVTCLLEGPSGSGKTALAATIGIDSDFPYVKIVSAETMIG 569

  Fly   581 FTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKL 645
            .:||.||.||.|:|:|||:|.:|.|::|::||||::..||||:||:..|.|:||||:.||||:||
plant   570 LSESTKCAHIVKVFEDAYKSPMSIIILDDIERLLEFIAIGPRFSNIISQTLMVLLKRLPPKGKKL 634

  Fly   646 LILCTSSRRDVLEEMEMLSAFTSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMAGKRLCI 710
            |:..|:|....||.:.:...| ||.|.......|:...||:..:|||.:::.|.|..:..    :
plant   635 LVFGTTSEVTFLESVGISDCF-SVTHSVPTLQKEDAKKVLNQLNLFSEDDVDSAAEALND----M 694

  Fly   711 GIKKLLALIDMIRQSE 726
            .|||:..||:|..|.|
plant   695 PIKKIYMLIEMAAQGE 710

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nsf2	NP_001287318.1	CDC48_N	9..89	CDD:215012	20/89 (22%)
		CDC48_2	117..>170	CDD:215011	9/52 (17%)
		AAA	259..404	CDD:214640	104/144 (72%)
		AAA	261..402	CDD:278434	100/140 (71%)
		AAA	543..674	CDD:214640	66/130 (51%)
		P-loop_NTPase	544..>613	CDD:304359	36/68 (53%)
NSF	NP_192400.2	CDC48_N	12..92	CDD:215012	20/85 (24%)
		CDC48_2	127..173	CDD:281000	9/53 (17%)
		Parvo_NS1	170..>270	CDD:305166	49/103 (48%)
		AAA	251..396	CDD:214640	104/144 (72%)
		AAA	253..394	CDD:278434	100/140 (71%)
		AAA	527..662	CDD:99707	69/135 (51%)
		AAA	532..656	CDD:214640	63/124 (51%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	218	1.000	Domainoid score	I732
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	552	1.000	Inparanoid score	I255
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D197562at2759
OrthoFinder	1	1.000	-	-		FOG0003053
OrthoInspector	1	1.000	-	-		otm2625
orthoMCL	1	0.900	-	-		OOG6_101440
Panther	1	1.100	-	-	LDO	PTHR23078
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X2410
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	12	11.830