Protein Alignment MCF2 and trio

DIOPT Version :9

• Sequence 1: XP_005262470.1
• Gene: MCF2 / 4168
• HGNCID: 6940
• Length: 1044
• Species: Homo sapiens
• Sequence 2: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Alignment Length: 1028
• Identity: 250/1028 - (24%)
• Similarity: 419/1028 - (40%)
• Gaps: 248/1028 - (24%)

Human   171 LTYIDDKQLTPELGGTLQYCHSE-------W--------IIFRNAIENFALTVKEMAQMLQSFGT 220
            |.|.|:......|..||:..:.:       |        :|.|  :..|....:.::..::.:..
  Fly   740 LEYADNTGSIAALEATLEKLNKQKVELEELWSARKFRADLILR--LRYFERDARLLSVQMEMWSE 802

Human   221 ELAETELPDDIPSIEEILAIRAERYHLLKNDITAVTKEGKILLTNLE------VPDTEGAVSSRL 279
            ||...:|..|....|:::.:..|....::|....|.::|:.||...|      :.|......:|:
  Fly   803 ELQHADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFENAGFISMADATHTAQARI 867

Human   280 ECHRQISGDWQTINKLLTQVHDMETAFDGFWEKHQLKMEQYLQLWKFEQDFQQLVT--------- 335
            |             .||..:.:.|...:...|..:.|:||.:||.:|:.|..|:::
  Fly   868 E-------------YLLDFLREREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAML 919

Human   336 -----------EVEFLL--NQQAELA-DVTGTIA-QVKQKIKKLEN--------LDENSQELLSK 377
                       |.|.|.  ::|.::| :.|.|.| |||.:...|.|        :||.|.::..|
  Fly   920 VASFVTPNSLQEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKK 984

Human   378 AQFVILHG---HKL---AANHHYALDLICQRCNELRYLSDILVNEIKAKRIQLSRTFKMHKLLQQ 436
            .|.::.:.   |||   :.|.:...:.:|.       :.|.|..|.|                 :
  Fly   985 WQQLVTYAEERHKLVTASINFYKTAEQVCS-------VLDSLEREYK-----------------R 1025

Human   437 ARQCCDEGECLLANQEIDKFQSKEDAQK---------ALQDIENFLEMALPFINYEPETLQY--- 489
            ....||.|......|.|.:..||...||         |.:..|.||:.|    |...:..||
  Fly  1026 EDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYA----NRSQQCYQYKGN 1086

Human   490 -------EFDVILSPELKV------QMKTI----QLKL-----------------------ENIR 514
                   :.|.:|:.|.:|      :.|::    |..|                       .|:.
  Fly  1087 CEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSNLV 1151

Human   515 SIFENQQAG-------FRNLADKHVRPIQFVVPTPENLVTSGTPFFSSKQVGVGYSFFQACKLFS 572
            .|.:.:..|       ||:.|.:....::.::...::||..|....|..:..|. |..|..|.||
  Fly  1152 GISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVA-SVDQRYKDFS 1215

Human   573 KG--------KKTWRQNQSNLKI-----EVVPDCQEKRSSGPS--SSLDNGNSLDV--------- 613
            ..        :|:...:|..|.:     ..:......|.|.|:  :.|::.|..:.
  Fly  1216 NRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLNSSNKENKEINEEKRKS 1280

Human   614 --LKNHVLNELIQTERVYVRELYTVLLGYRAEMDNPEMFDLMPPLLRNKKDILFGNMAEIYEFHN 676
              .|..::.||:||||.||.:|.|.:..:..|....:.   :|..|..::|::|||:.||:.||.
  Fly  1281 ARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKS---VPSALIGQEDVIFGNIKEIHHFHQ 1342

Human   677 DIFLSSLENCAHAPERVGPCFLERKDDFQMYAKYCQNKPRSETIWRKYSECAFFQECQRKLKHRL 741
            .|||..||.....||.||.||:.....|.||..||:|||.|..:..::.. :||:|.||:|:...
  Fly  1343 KIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGG-SFFEELQRRLEVDH 1406

Human   742 RLDSYLLKPVQRITKYQLLLKELLKYSKDCEGSALLKKALDAMLDLLKSVNDSMHQIAINGYIGN 806
            .|.:||:||||||||||||||:||...::..|.  :|:.|:.||::.|..||:||...:.....:
  Fly  1407 PLPAYLIKPVQRITKYQLLLKDLLSCCEESHGE--IKEGLEVMLNVPKKANDAMHLSLLENCDVS 1469

Human   807 LNELGKMIMQGGFSVWIGHKKGATKMKDLARFKPMQRHLFLYEKAIVFCKRRVESGEGSDRYPSY 871
            :::||::::|..|..|        ..|.:.| |..:|.:||:|..::|.|...||     ....|
  Fly  1470 VDKLGEVVLQDAFQAW--------DTKQIIR-KGRERRVFLFELYLLFAKEVKES-----NVVKY 1520

Human   872 SFKHCWKMDEVGITEYVKGDNRKFEIWYGEKEEV----YIVQASNVDVKMTWLKEIRNILLKQQE 932
            .||......::||||:::||..||.:|.|....:    .:::|::::.|..|:|::|.::   ||
  Fly  1521 QFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVM---QE 1582

Human   933 L------LTVKK-------------RKQQDQLTERDKFQISLQQNDEKQQGAFISTEETELEHTS 978
            .      ||:.|             |...:||||.|..:.||        .:|.|...|:.::..
  Fly  1583 TCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTENDHDRCSL--------ASFGSGNTTDSDNKL 1639

Human   979 TVVEVCEAIASVQAE--ANTVWTEASQSAEISEEPAEWSSNY----FYPTYDE 1025
            ...|....:|...|.  :|.:.....|..||.|.|.....::    ..|.:|:
  Fly  1640 GNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDD 1692

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
MCF2	XP_005262470.1	SEC14	48..188	CDD:214706	5/16 (31%)
		SPEC	328..519	CDD:238103	55/280 (20%)
		RhoGEF	615..792	CDD:238091	73/176 (41%)
		PH-like	799..930	CDD:302622	34/134 (25%)
		PH	842..927	CDD:278594	26/88 (30%)
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103	7/37 (19%)
		SPEC	782..997	CDD:238103	47/229 (21%)
		SPEC	897..1119	CDD:238103	54/249 (22%)
		SPEC	1125..1226	CDD:197544	16/101 (16%)
		RhoGEF	1287..1456	CDD:279015	72/174 (41%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	36/138 (26%)
		PH	1495..1580	CDD:278594	26/89 (29%)
		SH3_Kalirin_1	1643..1707	CDD:212786	11/50 (22%)
		RhoGEF	1945..2122	CDD:279015
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4240
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810