Protein Alignment CG32473 and ANPEP

DIOPT Version :10

• Sequence 1: NP_731787.1
• Gene: CG32473 / 41636
• FlyBaseID: FBgn0052473
• Length: 1025
• Species: Drosophila melanogaster
• Sequence 2: NP_001141.2
• Gene: ANPEP / 290
• HGNCID: 500
• Length: 967
• Species: Homo sapiens
• Alignment Length: 1016
• Identity: 337/1016 - (33%)
• Similarity: 526/1016 - (51%)
• Gaps: 116/1016 - (11%)

  Fly    46 LVLCGFLLG---ALVYVGKNIAEEHQRQQHANASQWALNSTIRTVYVDRDQLLSTKPIISVLSDN 107
            |.:.|.|||   ....:..::....::.::||:|..|                ||.|..|
Human    11 LGILGILLGVAAVCTIIALSVVYSQEKNKNANSSPVA----------------STTPSAS----- 54

  Fly   108 RFYTTQVPTSKKSSIMATVAPAVKAASKVLQNLGFRLPKQIKPSKYRLHLRPDLERKN-----YS 167
               .|..|.|      ||.....||.::      :|||..:||..||:.|||.|...:     :.
Human    55 ---ATTNPAS------ATTLDQSKAWNR------YRLPNTLKPDSYRVTLRPYLTPNDRGLYVFK 104

  Fly   168 GNISISLQVLEPIAFIPVHVKQLNVSTVEVKRL---DESGAPLKDITPTLTFAHPEFEYWVTEFE 229
            |:.::.....|....|.:|.|:||.:..:..|:   ...|:...||..| ....|. ||.|...:
Human   105 GSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKT-ELVEPT-EYLVVHLK 167

  Fly   230 QPL-EAGNYSLLLNFTGSLVDRITGMYQSSYLDKLKNRSRSIISTKFEPTYARQAFPCFDEPALK 293
            ..| :...|.:...|.|.|.|.:.|.|:|.|::  .|..:.:.:|:.:...||::||||||||:|
Human   168 GSLVKDSQYEMDSEFEGELADDLAGFYRSEYME--GNVRKVVATTQMQAADARKSFPCFDEPAMK 230

  Fly   294 AQFTITVARPSGDEYHVLSNM-------PVASEYVDGDITEVTFAETVPMSTYLAAFVVSDFQYK 351
            |:|.||:..|.  :...||||       |: .|..:.::||  |..|..|||||.||:||:|.|.
Human   231 AEFNITLIHPK--DLTALSNMLPKGPSTPL-PEDPNWNVTE--FHTTPKMSTYLLAFIVSEFDYV 290

  Fly   352 ETTVEGTSIALKVYAPPAQVE--KTQYALDTAAGVMAYYINYFNVSYALPKLDLVAIPDFVSGAM 414
            |... ...:.::::|.|:.:.  ...|||:....::.::..:::..|.|||.|.:.:|||.:|||
Human   291 EKQA-SNGVLIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAM 354

  Fly   415 ENWGLVTFRETALLYDESTSSSVNKQRVAIVVAHELAHQWFGNLVTMNWWNDLWLNEGFASFLEY 479
            |||||||:||.:||:|..:|||.||:||..|:||||||||||||||:.|||||||||||||::||
Human   355 ENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEY 419

  Fly   480 KGVKQMHPEWDMDNQFVIEELHPVLTIDATLASHPI---VKSIESPAEITEYFDTITYSKGAALV 541
            .|.....|.|::.:..|:.:::.|:.:||..:|||:   ...|.:||:|:|.||.|:|||||:::
Human   420 LGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKGASVL 484

  Fly   542 RMLENLVGEEKLRNATTRYLVRHIYSTATT-----EDYLTAVEEEEGLEF--DVKQIMQTWTEQM 599
            |||.:.:.|:..:.....||  |.::...|     .|:|........::.  .|:.||..||.||
Human   485 RMLSSFLSEDVFKQGLASYL--HTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQM 547

  Fly   600 GLPVVEVEKSGSTYKLTQKRFLANEDDYAAEAEASSFNYRWSIPITYTSS---------INSEVQ 655
            |.||:.|:.|..|  |:|:.||.:.|...  ...|.|||.|.:|||....         |:...|
Human   548 GFPVITVDTSTGT--LSQEHFLLDPDSNV--TRPSEFNYVWIVPITSIRDGRQQQDYWLIDVRAQ 608

  Fly   656 SLIFNHNDNEATITLPEEASWIKINTNQVGYYRVNYGSEQWAELISALKNSRETFSTADRAHLLN 720
            :.:|:.:.||          |:.:|.|..|||||||..|.|.::.:.|:.........:||.::|
Human   609 NDLFSTSGNE----------WVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQIIN 663

  Fly   721 DANTLAAAGQLNYSVALDLISYLESEQDYVPWSVGTSALATLRNRVYYTDLYTNYTTYARKLLTP 785
            ||..||:|.::..::||:...:|..|:.|:||....|:|:..:.....:::|.....|.:|.:||
Human   664 DAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKNYLKKQVTP 728

  Fly   786 IVEKVTF--------TVAADHLENRLRIKVLSSACSLGHESSLQQAVTLFNQWLASPETRP-NPD 841
            :.  :.|        .:..:.::....:..:|:|||.|.....:....||.||:.:|...| :|:
Human   729 LF--IHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWMENPNNNPIHPN 791

  Fly   842 IRDVVYYYGLQQVNTEAAWDQVWKLYLDESDAQEKLKLMNCLTAVQVPWLLQRYINWAWDESNVR 906
            :|..||...:.| ..|..||..|:.:.:.:...|..||...|...:..|:|.||:::..:...:|
Human   792 LRSTVYCNAIAQ-GGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWILNRYLSYTLNPDLIR 855

  Fly   907 RQDYFTLLGYISTNPVGQSLVWDYVRENWEKLVDRFGINERTLGRLIPTITARFSTETKLEEMQQ 971
            :||..:.:..|:.|.:||.||||:|:.||:||.:.:|....:...||..:|.|||||.:|::::|
Human   856 KQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSNLIQAVTRRFSTEYELQQLEQ 920

  Fly   972 FFAKYPEA--GAGTAARQQALEAVKANIKWLAANKAQVGEW 1010
            |.....|.  |:||.|.:||||..||||||:..||..|.:|
Human   921 FKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQW 961

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG32473	NP_731787.1	M1_APN-Q_like	151..597	CDD:341064	174/473 (37%)
		ERAP1_C	676..995	CDD:463368	101/329 (31%)
ANPEP	NP_001141.2	Cytosolic Ser/Thr-rich junction	33..68		13/64 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	40..62		11/51 (22%)
		Metalloprotease	69..967		319/928 (34%)
		M1_APN-Q_like	84..545	CDD:341064	174/472 (37%)
		Necessary and sufficient to mediate interaction with HCoV-229E. /evidence=ECO:0000269|PubMed:1350662, ECO:0000269|PubMed:8887485	288..295		2/7 (29%)
		ERAP1_C	619..946	CDD:463368	101/329 (31%)