Protein Alignment timeout and TIMELESS

DIOPT Version :10

• Sequence 1: NP_524341.3
• Gene: timeout / 41615
• FlyBaseID: FBgn0038118
• Length: 1384
• Species: Drosophila melanogaster
• Sequence 2: NP_003911.2
• Gene: TIMELESS / 8914
• HGNCID: 11813
• Length: 1208
• Species: Homo sapiens
• Alignment Length: 1254
• Identity: 432/1254 - (34%)
• Similarity: 701/1254 - (55%)
• Gaps: 97/1254 - (7%)

  Fly     1 MSILLADIDATCAALGYSDGQKYQAEPDAAEGLKHLIWILRRDLDNHEYRRHLGRSKVLQTDLVY 65
            :.::..::.|||:||||.:|..|..|||..|.:|.||..||.:.:..:.|:.||.:::||:||:.
Human     3 LHMMNCELLATCSALGYLEGDTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLP 67

  Fly    66 MLPDYVHHEE--LSDLLIRLLVILTNPTLLLYREGAPKDNHGRKVFMELIDILQGYKAAFAKDKV 128
            :|..  ||::  |.|.:|||:|.||.|.||.: ...||:...|..|::::..||.||.|||.:|.
Human    68 ILTQ--HHQDKPLFDAVIRLMVNLTQPALLCF-GNLPKEPSFRHHFLQVLTYLQAYKEAFASEKA 129

  Fly   129 WSALFEKLKQALEIAFAIRSEEQNLLIERILVLVRNVLQVPANPEAECRADNDASLHDQVIWALH 193
            :..|.|.|.:.|::.:..|.||.||||||||:||||:|.|||:.:.|.:.|:|||.|||::||:|
Human   130 FGVLSETLYELLQLGWEERQEEDNLLIERILLLVRNILHVPADLDQEKKIDDDASAHDQLLWAIH 194

  Fly   194 QTGMLDLVLFVISSPDEEQFHLHGLEIVCLLFREQSAESLADASLQRSLSEKQRDQQELLAARRR 258
            .:|:.||:||:.||..|||:.||.||||.|:||:|:.|.||.....|...|:..|..||...|:|
Human   195 LSGLDDLLLFLASSSAEEQWSLHVLEIVSLMFRDQNPEQLAGVGQGRLAQERSADFAELEVLRQR 259

  Fly   259 ERARRQAR--PPPGRHSRFGGTYVIRNMKSVSDRDVICHQALERVSSIDFDREKQQQK-RSHRHI 320
            |.|.::.|  ....|||||||:|:::.:||:.:||:|.|:.|..:.:...|..||.:| ...|..
Human   260 EMAEKKTRALQRGNRHSRFGGSYIVQGLKSIGERDLIFHKGLHNLRNYSSDLGKQPKKVPKRRQA 324

  Fly   321 QEEAQVTRRSAFTVRLCLREYCIEVLRSAYNTLVRQVRRVLERNAGSSSHDDSYLLWAIRFFMEF 385
            ..|..:.||||..|||.||::|.|.|.:.||.|:..|:..|.|.. :..||::|.:||:.|||.|
Human   325 ARELSIQRRSALNVRLFLRDFCSEFLENCYNRLMGSVKDHLLREK-AQQHDETYYMWALAFFMAF 388

  Fly   386 NRLSGLQLQLVSESLSVQCFHWVLTRMQHDMDMIVSDKKQARLWAKRLHVALLTFRELLQSLLAL 450
            ||.:..:..||||:|||:.||::...:.:..:|:::|:|:|..||:|:|:||..::|||.::..:
Human   389 NRAASFRPGLVSETLSVRTFHFIEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEM 453

  Fly   451 QKLKDDNNARALFDMLLNNVCYVLEYRETVLHLLMNYNEAHSTKVFLRDVVETANVFIKMMERFC 515
            . :..|...|....::.||:.||:||||..|.|...::|....:.||||:|||.::|:||:||||
Human   454 D-ISPDEAVRESSRIIKNNIFYVMEYRELFLALFRKFDERCQPRSFLRDLVETTHLFLKMLERFC 517

  Fly   516 QD--SVVVQDKKRGRGASRKK--KQAATKSKPPAAPQPTEEELSSKWAELATEV-CSLLSTELEM 575
            :.  ::|||:|::.|...:||  .||......|::|    ||:.:.|..||.:: |...::||.|
Human   518 RSRGNLVVQNKQKKRRKKKKKVLDQAIVSGNVPSSP----EEVEAVWPALAEQLQCCAQNSELSM 578

  Fly   576 PEDEQPLPFDAASEKSIDDQREDCMIRINKLLRSEKLDQAIALLRAAREVWPENEVFGAISAAPE 640
               :..:|||||||..:::||.:.|:||...|.:.:..||:.|||:|||||||.:|||:...:||
Human   579 ---DSVVPFDAASEVPVEEQRAEAMVRIQDCLLAGQAPQALTLLRSAREVWPEGDVFGSQDISPE 640

  Fly   641 DELLLLREIFMSNITTVESEDKENEKDQKQDEYEDEDDDLDNEEYEEQDNGLTEKTFKFDDFARR 705
            :|:.||::|..:.:...:..::...::::::|.|:|:        |.|...::||.|.|.|:.:|
Human   641 EEIQLLKQILSAPLPRQQGPEERGAEEEEEEEEEEEE--------ELQVVQVSEKEFNFLDYLKR 697

  Fly   706 LLNPKIVRACTLVLSDWADIPTRSLKAAVTILHRIAYGCKCAGMLFQAKLFRIFQQVFSVERDVH 770
            .....:|||..|:|..:......:....|.:|||:|:..|...:|||..:|.:|.::.|......
Human   698 FACSTVVRAYVLLLRSYQQNSAHTNHCIVKMLHRLAHDLKMEALLFQLSVFCLFNRLLSDPAAGA 762

  Fly   771 QEELRRLAIFVVRKFVEVAPTNPKIYAELLFYKGIREANELESGY---CDAYEAGTKGAWTEEQE 832
            .:||...|.:::.||..:|..|.|.:.||||:|......|:..||   .|...:.....|:.|:|
Human   763 YKELVTFAKYILGKFFALAAVNQKAFVELLFWKNTAVVREMTEGYGSLDDRSSSRRAPTWSPEEE 827

  Fly   833 SELRFLFEENQRNPETDKDVIDWILDNLVDKTRNRRTVLKKLKELGLLFKAPTKRSTKSAQ-SGK 896
            :.||.|:..|:  ....:||::.||.:|....|.|:.::..|.::||      ..|.|..| .|.
Human   828 AHLRELYLANK--DVEGQDVVEAILAHLNTVPRTRKQIIHHLVQMGL------ADSVKDFQRKGT 884

  Fly   897 NV--WQPEEDDELRSLYDQHRIEPDCLERLVNEFAERRSKQQIIKRMLQIHLIADK--------- 950
            ::  |..:::.||:.|:::.|...|.|..::.....:||:.:|:.::|.:.|:|::
Human   885 HIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQK 949

  Fly   951 ---SEILPAKKGRGKDKPKKDVEMEGEGDEFDFAEEPMFEDQGYKKPKSKPKKVQKRQMVRTPLD 1012
               |.|||......||..::|:|.|....|.|..||   |:.|     |:.::|| ..:|.:..:
Human   950 KLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEE---EEGG-----SEAEQVQ-GSLVLSNEN 1005

  Fly  1013 VGTIRALIGQVDSEKYQSAIEWLQECLQDASEDTEEAVEDDDG----VPLLPLMENQKNAMEDGD 1073
            :|.      .:..|.:...:.|||.||..|::|.||     ||    |||:||.|..:.|||:..
Human  1006 LGQ------SLHQEGFSIPLLWLQNCLIRAADDREE-----DGCSQAVPLVPLTEENEEAMENEQ 1059

  Fly  1074 FQKVLVALGMQPPISGMEAYWRIPIYLNSADLILRSKILAGEEVDAEPEDEAADDEDGEEAEESD 1138
            ||::|..||::||.||.|.:||||..|:...|...:..|:..|.:.:.:.|......||:.  ||
Human  1060 FQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQG--SD 1122

  Fly  1139 EEEDFLEKHSRQRKENIATQQQRKLDSLMFNQSDDETEQRAPPKAKDKPKASEKKAKRKVKGKAK 1203
            ||.  .::|..|....:....::|..  :.:..:::...:.|.||..|        ||::....:
Human  1123 EEH--CKEHRAQALRALLLAHKKKAG--LASPEEEDAVGKEPLKAAPK--------KRQLLDSDE 1175

  Fly  1204 ESKDCNDDGTDKA-ELEKP 1221
            |.::  |:|.::| ||..|
Human  1176 EQEE--DEGRNRAPELGAP 1192

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
timeout	NP_524341.3	TIMELESS	22..281	CDD:461444	118/262 (45%)
		TIMELESS_C	1019..1105	CDD:461529	36/89 (40%)
TIMELESS	NP_003911.2	Required for homodimerization and for interaction with CRY1 and CHEK1. /evidence=ECO:0000250|UniProtKB:Q9R1X4	1..309		133/308 (43%)
		TIMELESS	24..284	CDD:461444	118/262 (45%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	655..679		3/31 (10%)
		DNA-binding domain. /evidence=ECO:0000269|PubMed:32705708	816..954		34/145 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	971..994		9/30 (30%)
		Interaction with PARP1. /evidence=ECO:0000269|PubMed:26344098	1000..1098		39/108 (36%)
		TIMELESS_C	1006..1092	CDD:461529	37/96 (39%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1091..1131		11/43 (26%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1143..1208		14/62 (23%)
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:31138685	1182..1199		5/11 (45%)