DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sim and epas1b

DIOPT Version :9

Sequence 1:	NP_524340.2	Gene:	sim / 41612	FlyBaseID:	FBgn0004666	Length:	688	Species:	Drosophila melanogaster
Sequence 2:	NP_001034895.2	Gene:	epas1b / 555192	ZFINID:	ZDB-GENE-060607-11	Length:	834	Species:	Danio rerio

Alignment Length:	846	Identity:	217/846 - (25%)
Similarity:	334/846 - (39%)	Gaps:	245/846 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 RKDCYESRLHDIAKTCAMKEKSKNAARTRREKENTEFCELAKLLPLPAAITSQLDKASVIRLTTS 72
            :|.|...|         .||||::|||.||.||...|.|||..||||.:|:|.|||||::||..|
Zfish     7 KKRCSSER---------RKEKSRDAARCRRSKETEVFYELAHHLPLPHSISSHLDKASIMRLAIS 62

  Fly    73 YLKMRQVFPDGLGEAWGSSPAMQRGATIKELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGL 137
            :|:.|::...|.     ::|.....|. :.:.|..|::|.|||.||..||.::::||..:..:||
Zfish    63 FLRTRKLVNSGY-----NTPTEMTDAD-RLMDSWYLKSLGGFITVVTSDGDMIFLSENINKFMGL 121

  Fly   138 SQVELTGNSIFEYIHNYDQDEMNAILSLHPHINQHPLAQTHTPIGSPNGVQHPSAYDHDRGSHTI 202
            :||||||:|||::.|..|.:|:...|||...|.:                         :|.. :
Zfish   122 TQVELTGHSIFDFTHPCDHEEIRENLSLKAGIGK-------------------------KGKE-L 160

  Fly   203 EIEKTFFLRMKCVLAK--RNAGLTTSGFKVIHCSGYLK------ARIYPDRGDGQGSLIQNLGLV 259
            ..|:.||:||||.:..  |...|.::.:||:||:|:||      ||:.      .|.....|..|
Zfish   161 STERDFFMRMKCTVTNRGRTVNLKSASWKVLHCTGHLKVCNGCPARVL------CGFKEPPLTCV 219

  Fly   260 A-----VGHSLPSSAITEIKLHQNMFMFRAKLDMKLIFFDARVSQLTGYEPQDLIEKTLYQYIHA 319
            .     :.|  ||:..|  .|....|:.|..:|||..:.|.||::|.||.|:||:.::.|::.||
Zfish   220 VMMCEPIVH--PSNIDT--PLDSKTFLSRHSMDMKYTYCDERVTELMGYNPEDLLGRSAYEFYHA 280

  Fly   320 ADIMAMRCSHQILLYKGQVTTKYYRFLTKGGGWVWVQSYATLVHNSRSSREVFIVSVNYVLSERE 384
            .|...:..|||.|..|||..:..||.|.|.||:|||::..|:::|||:|:...||.||||||:.|
Zfish   281 LDAENVTKSHQNLCTKGQAVSGQYRMLAKNGGYVWVETRGTVIYNSRNSQPQCIVCVNYVLSDVE 345

  Fly   385 VKDLVLNEIQT----------------------------GVVKREPISPAAQAAQAAQAAQAAQA 421
            .|.|:.:..||                            ..:|.||......|........:...
Zfish   346 EKSLIFSMDQTESLFKPHKLNGFFSPKEALGSDPADLLFTKLKEEPEDLTQLAPTPGDTIISLDF 410

  Fly   422 AQAAQAAH--------VAQAVQAQV----------VVVPQQSVVVQPQCAGATGQPVGPGTPVSL 468
            .|:....|        |||.|...|          .:....:....||.|     |....||...
Zfish   411 GQSQYEEHTVYNKVSSVAQTVSHPVHDGHRTSYSGEMAKMAATFSVPQSA-----PPSSATPSLS 470

  Fly   469 ALSASPKLDPYFEP------------------------------------------------ELP 485
            :.|.....|.|:.|                                                :..
Zfish   471 SCSTPSSPDDYYTPVDSDLKVELTEKLFSLDTQEAKTSRNQETDLSDLDLETLAPYIPMDGEDFQ 535

  Fly   486 LQPAVTPVPPTNNSSSSSN-----NNNGVWH------HHHVQQQQQSGSMDHDSLS--------- 530
            |.|.....||:...:..:|     |...::.      ..|.|.:..||. |..:::
Zfish   536 LNPICPEEPPSEIGTLGTNQQCFSNITSLFQPLSSPSAAHYQPKMSSGG-DKQNINGGSVESWPP 599

  Fly   531 --YTQ---LYPPLNDLVVSSSSSVGG----------GTASSAG--GGSSASASSSGVYSTEM--- 575
              |::   ..||.:|...:..||:||          ...|.||  ...:|..|..|:.:..|
Zfish   600 VPYSRDPMQMPPYHDPASTPLSSMGGRQNLQWPPDPPLPSKAGMMDPLAAKRSCQGMPANRMAPF 664

  Fly   576 ----------QYPDTTTGNLYYNNNNHYYYDYDATVDVATSMIRPFSANSNSCSSSSESE----R 626
                      .|.||:                .|.:.:|.|..|.||..:.:.:..::|:    :
Zfish   665 MQRPMENFVQNYRDTS----------------PARLALANSFKRSFSQMAMAETPPTKSQQTVWK 713

  Fly   627 QLSTGNASIVNETSPSQTTYSDLSHNFELSYFSDNSSQQHQHQQQQ----QHLMEQQHLQYQYAT 687
            :|...:.:::...|.|.:..||.|       .:.|....|||::.|    |:....:|.:.|:..
Zfish   714 KLRHESCAVMERKSLSSSALSDKS-------MAHNGGMDHQHRKSQYSGNQNGQPTKHYREQFCN 771

  Fly   688 W 688
            :
Zfish   772 Y 772

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sim	NP_524340.2	HLH	26..78	CDD:238036	30/51 (59%)
		PAS	102..215	CDD:279347	37/112 (33%)
		PAS	102..163	CDD:214512	26/60 (43%)
		PAS	278..376	CDD:238075	41/97 (42%)
		PAS_3	291..377	CDD:285623	36/85 (42%)
epas1b	NP_001034895.2	HLH	12..68	CDD:238036	31/64 (48%)
		PAS	92..147	CDD:214512	25/54 (46%)
		PAS	238..337	CDD:238075	41/98 (42%)
		PAS_3	251..338	CDD:285623	36/86 (42%)
		HIF-1	511..543	CDD:288296	2/31 (6%)
		HIF-1a_CTAD	798..833	CDD:285931
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3558
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000232
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.810