DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sim and hif1al2

DIOPT Version :9

Sequence 1:	NP_524340.2	Gene:	sim / 41612	FlyBaseID:	FBgn0004666	Length:	688	Species:	Drosophila melanogaster
Sequence 2:	NP_001012371.2	Gene:	hif1al2 / 497283	ZFINID:	ZDB-GENE-050211-2	Length:	663	Species:	Danio rerio

Alignment Length:	710	Identity:	173/710 - (24%)
Similarity:	280/710 - (39%)	Gaps:	227/710 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 RRVRKDCYESRLHDIAKTCAMKEKSKNAARTRREKENTEFCELAKLLPLPAAITSQLDKASVIRL 69
            ::.|:.|.|.|     |||     |:.||::||:||:..|.||..||||..::..|.||||||||
Zfish    26 QKKRRVCTEWR-----KTC-----SRLAAQSRRKKESQLFKELTALLPLDLSMDGQRDKASVIRL 80

  Fly    70 TTSYLKMRQVFPDGLGEAWGS-----SPAMQRGATIKELGSHLLQTLDGFIFVVAPDGKIMYISE 129
            |.:||.:|     ||.:...|     ||.:...:..:||   |...|.||:.:::.:||:::.::
Zfish    81 TIAYLHLR-----GLLDTTDSLMKRPSPGVSDKSHEREL---LDSALGGFVVLLSLNGKVIFATK 137

  Fly   130 TASVHLGLSQVELTGNSIFEYIHNYDQDEMNAILSLHPHINQHPLAQTHTPIGSPNGVQHPSAYD 194
            ..:.|.|::|::|.|.|:.|::|..||.|:..||:              ..||:    |.|...:
Zfish   138 GLTTHTGINQMDLIGRSLLEFLHPCDQMEVKDILT--------------RLIGN----QEPQKCE 184

  Fly   195 HDRGSHTIEIEKTFFLRMKCVLAKRNAGLTTSGFKVIHCSGYLKARIYPDRGDGQGSLIQNLGLV 259
                         .|||:|..:   |..||  |:|:|.|||..::...|    |...|:....:
Zfish   185 -------------VFLRIKKAM---NTKLT--GWKIIQCSGKKRSSATP----GSSCLVLECRV- 226

  Fly   260 AVGHSLPSSAITEI--KLHQNMFMFRAKLDMKLIFFDARVSQLTGYEPQDLIEKTLYQYIHAADI 322
                 ||...|||:  .|:...||.....||...:..:||.:|.|:...||:.:::|||.|.:|.
Zfish   227 -----LPIQEITEVDEALNACTFMSVHSPDMTFTYCHSRVVKLIGFRDTDLLGQSVYQYYHPSDC 286

  Fly   323 MAMRCSHQILLYKGQVTTKYYRFLTKGGGWVWVQSYATLVHNSRSSREVFIVSVNYVLSEREVKD 387
            ..:|.:|..||.||||:|..||.|.:.||:||.::.|:||.||::.....:|.:||:|||.|..:
Zfish   287 QQIRKAHICLLSKGQVSTGKYRLLHRYGGYVWAETDASLVCNSQTGVPESVVCINYILSEVEQPN 351

  Fly   388 LVLNEIQTGVVKREPISPAAQAAQAAQAAQAAQAAQAAQAAHVAQAVQAQVVVVPQQSVVVQPQC 452
            |                                                             |..
Zfish   352 L-------------------------------------------------------------PFL 355

  Fly   453 AGATGQPVGPGTPVSLALSASPKLDPYFEPELPLQPAVTPVPPTNNSS-----SSSNNNNGVWHH 512
            ...|.|.:.|     .:.|..|:.:|            .|.||:....     ..||.:|....|
Zfish   356 LEKTEQLLKP-----TSSSYCPETEP------------APFPPSRGQEGEKDLKESNEHNSFITH 403

  Fly   513 HHVQQQQQSG-------------------------SMDHDSLSYTQLYPPLN--DLVVSSSSSVG 550
                .:|:.|                         .:|.|||:   .|.|::  |.:::.
Zfish   404 ----LKQRDGDIQEVECTEIPHNTEGCYDFNCDQCELDLDSLA---PYIPMHGEDFLLTP----- 456

  Fly   551 GGTASSAGGGSSASASSSGVYSTEMQYPDTTTGNLYYNNNNHYYYDYDATVDVATSMIRPFSANS 615
                               :....|..|:..|.....::.:.   :.|::.......::..::..
Zfish   457 -------------------IIDRAMDMPELNTCRPSCSSPSQ---NLDSSEGTTVLPVKSLNSTQ 499

  Fly   616 NSCSSSSESERQLSTGNAS---------IVNETSPSQTTYSDLSHNFELSYFSDNSSQQH 666
            .|.|:|.:::|...|...|         :||:.| ....|....||::|  ||..:...|
Zfish   500 QSVSNSFQTKRISDTNLISTKQWSDSFDLVNKVS-KVYDYDPKQHNYQL--FSSETKANH 556

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sim	NP_524340.2	HLH	26..78	CDD:238036	26/51 (51%)
		PAS	102..215	CDD:279347	29/112 (26%)
		PAS	102..163	CDD:214512	19/60 (32%)
		PAS	278..376	CDD:238075	37/97 (38%)
		PAS_3	291..377	CDD:285623	33/85 (39%)
hif1al2	NP_001012371.2	HLH	36..86	CDD:238036	29/59 (49%)
		PAS	111..>184	CDD:279347	25/93 (27%)
		PAS	119..225	CDD:238075	38/145 (26%)
		PAS	243..340	CDD:238075	37/96 (39%)
		PAS_3	254..341	CDD:285623	33/86 (38%)
		HIF-1	429..461	CDD:288296	7/58 (12%)
		HIF-1a_CTAD	620..656	CDD:285931
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3558
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000232
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810