DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment sim and hif1ab

DIOPT Version :9

Sequence 1:	NP_524340.2	Gene:	sim / 41612	FlyBaseID:	FBgn0004666	Length:	688	Species:	Drosophila melanogaster
Sequence 2:	NP_001296971.1	Gene:	hif1ab / 393202	ZFINID:	ZDB-GENE-040426-706	Length:	777	Species:	Danio rerio

Alignment Length:	731	Identity:	199/731 - (27%)
Similarity:	315/731 - (43%)	Gaps:	172/731 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    26 KEKSKNAARTRREKENTEFCELAKLLPLPAAITSQLDKASVIRLTTSYLKMRQVFPDGLGEAWGS 90
            ||||::|||:||.||:..|.|||..||||..:||.|||||::|||.|||:||::......|
Zfish    18 KEKSRDAARSRRGKESEVFYELAHQLPLPHNVTSHLDKASIMRLTISYLRMRKLLNSDEKE---- 78

  Fly    91 SPAMQRGATIKELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIFEYIHNYD 155
              ..:......:|....|:.|:||:.|::.||.::|:||..|..:||:|.:|||:||||:.|..|
Zfish    79 --EKEENELESQLNGFYLKALEGFLMVLSEDGDMVYLSENVSKSMGLTQFDLTGHSIFEFSHPCD 141

  Fly   156 QDEMNAILSLHPHINQHPLAQTHTPIGSPNGVQHPSAYDHDRGSHTIE---IEKTFFLRMKCVLA 217
            .:|:..:|.                              |..||...:   .|::|||||||.|.
Zfish   142 HEELREMLV------------------------------HRTGSKKTKEQNTERSFFLRMKCTLT 176

  Fly   218 K--RNAGLTTSGFKVIHCSGYLKARIYPDRGDGQGSLIQNLG-LVAVGHSLPSSAITEIKLHQNM 279
            .  |...:.::.:||:||:|:::.....:..:..|.....:. ||.:...:|..:..|:.|....
Zfish   177 SRGRTVNIKSATWKVLHCAGHVRVHEGSEASEDSGFKEPPVTYLVLICEPIPHPSNIEVPLDSKT 241

  Fly   280 FMFRAKLDMKLIFFDARVSQLTGYEPQDLIEKTLYQYIHAADIMAMRCSHQILLYKGQVTTKYYR 344
            |:.|..||||..:.|.|:::|.||||.||:.:::|:|.||.|...:..:|..|..|||.||..||
Zfish   242 FLSRHTLDMKFSYCDERITELMGYEPDDLLNRSVYEYYHALDSDHLTKTHHNLFAKGQATTGQYR 306

  Fly   345 FLTKGGGWVWVQSYATLVHNSRSSREVFIVSVNYVLSEREVKDLVLNEIQT----GVVKREP--- 402
            .|.|.||:|||::.||:::|.::|:...||.||||||.....|:||:..||    ..|::|.
Zfish   307 MLAKKGGFVWVETQATVIYNPKNSQPQCIVCVNYVLSGIVEGDVVLSLQQTVTEPKAVEKESEET 371

  Fly   403 ------------ISPAAQAAQAAQAAQAAQAAQAAQAAHVAQAVQAQVVV--------------- 440
                        ..|.:.......:....:..:..:|..|........::
Zfish   372 EEKTSELDILKLFKPESLNCSLESSTLYNKLKEEPEALTVLAPAAGDAIISLDFNNSDSDIQLLK 436

  Fly   441 -VPQQSVVVQPQCAGATGQPVGPGTPVSLALSASPKLDPYFEPELPLQPAVTPVP-PTNNSSSSS 503
             ||..:.|:.|..:......:.|.|| |.::.|..||:...| :.|...|...|| |||..|:|.
Zfish   437 EVPLYNDVMLPSSSEKLPLSLSPLTP-SDSIPALTKLETGGE-DFPFSSASDRVPDPTNTPSTSG 499

  Fly   504 NNNNGVWHHHHVQQQQQSGSMDH------------------------------------DSLSYT 532
            ..::|           .:..||:                                    :.|...
Zfish   500 LGSSG-----------PNSPMDYGFPVEPDISSEFKLDLVEKLFAIDTEAKTPFSTQPMEDLDLE 553

  Fly   533 QLYP--------------PLNDL-----VVSSSSSVGGGTASSAGGGSSASASSSGVYSTEMQYP 578
            .|.|              ||:.|     .||:.||:.....||.  .|.||::||.|   :.:..
Zfish   554 MLAPYIPMDDDFQLRIPSPLDPLPSATHSVSAMSSLFQPLPSSP--ASPASSTSSTV---KQEAS 613

  Fly   579 DTTTGNLYYNNNNHYYYDYDATVDVATSMIRPFSANSNSC---SSSSESERQLSTGNASIVNETS 640
            ......|:            ...:|.::.:.|||.:.::.   ||:.:|..||:.      .|.|
Zfish   614 SRAPSPLH------------LLQEVCSAPVSPFSGSRDASPVRSSTPQSSSQLNN------REMS 660

  Fly   641 PSQTTYSDLSHNFELS 656
            |....:.::....:|:
Zfish   661 PKMLAFQNIQRKRKLN 676

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sim	NP_524340.2	HLH	26..78	CDD:238036	33/51 (65%)
		PAS	102..215	CDD:279347	36/115 (31%)
		PAS	102..163	CDD:214512	25/60 (42%)
		PAS	278..376	CDD:238075	42/97 (43%)
		PAS_3	291..377	CDD:285623	36/85 (42%)
hif1ab	NP_001296971.1	HLH	14..74	CDD:238036	34/55 (62%)
		PAS	94..149	CDD:214512	24/54 (44%)
		PAS	239..338	CDD:238075	42/98 (43%)
		PAS_3	254..339	CDD:285623	36/84 (43%)
		HIF-1	543..574	CDD:288296	4/30 (13%)
		HIF-1a_CTAD	740..776	CDD:285931
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3558
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000232
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810