DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sim and Epas1

DIOPT Version :9

Sequence 1:	NP_524340.2	Gene:	sim / 41612	FlyBaseID:	FBgn0004666	Length:	688	Species:	Drosophila melanogaster
Sequence 2:	XP_038967774.1	Gene:	Epas1 / 29452	RGDID:	68404	Length:	885	Species:	Rattus norvegicus

Alignment Length:	699	Identity:	190/699 - (27%)
Similarity:	297/699 - (42%)	Gaps:	164/699 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    26 KEKSKNAARTRREKENTEFCELAKLLPLPAAITSQLDKASVIRLTTSYLKMRQVFPDGLGEAWGS 90
            ||||::|||.||.||...|.|||..||||.:::|.|||||::||..|:|:..::......|....
  Rat    27 KEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESE 91

  Fly    91 SPAMQRGATIKELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIFEYIHNYD 155
            :.|.|      ::.:..|:.|:|||.||..||.::::||..|..:||:||||||:|||::.|..|
  Rat    92 AEADQ------QMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCD 150

  Fly   156 QDEMNAILSLHPHINQHPLAQTHTPIGSPNGVQHPSAYDHDRGSHTIEIEKTFFLRMKCVLAK-- 218
            .:|:...|:|          :|.:..|..|              ..:..|:.||:||||.:..
  Rat   151 HEEIRENLTL----------KTGSGFGKKN--------------KDMSTERDFFMRMKCTVTNRG 191

  Fly   219 RNAGLTTSGFKVIHCSGYLKARIY---PDRGDGQG---SLIQNLGLVAVGHSLPSSAITEIKLHQ 277
            |...|.::.:||:||:|  :.|:|   |......|   .|:..|.::......||.  .:|.|..
  Rat   192 RTVNLKSATWKVLHCTG--QVRVYNNCPPHSSLCGYKEPLLSCLIIMCEPIQHPSH--MDIPLDS 252

  Fly   278 NMFMFRAKLDMKLIFFDARVSQLTGYEPQDLIEKTLYQYIHAADIMAMRCSHQILLYKGQVTTKY 342
            ..|:.|..:|||..:.|.|:.:|.||.|::|:.::.|::.||.|...|..|||.|..||||.:..
  Rat   253 KTFLSRHSMDMKFTYCDDRILELVGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQ 317

  Fly   343 YRFLTKGGGWVWVQSYATLVHNSRSSREVFIVSVNYVLSEREVKDLVLNEIQTGVVKREPISPAA 407
            ||.|.|.||:||:::..|:|:|.|:.:...|:.|||||||.|..|:|.:..||     |.:....
  Rat   318 YRMLAKHGGYVWLETQGTVVYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQT-----ESLFKPH 377

  Fly   408 QAAQAAQAAQAAQAAQAAQAAHVAQAVQAQVVVVPQQSVVVQPQCAGATGQPVGPGTPVSLALSA 472
            ..|..:....:...|.:.::.::...::.
  Rat   378 LMAMNSIFDSSDDVALSEKSNYLFTKLKE------------------------------------ 406

  Fly   473 SPKLDPYFEPELPLQPAVTPVPPTNNSSSSSNNNNGVWHHHHVQQQQQSGSMDHDSLSYTQLYPP 537
                    |||...|.|.||.....:....|.|                  .|..|.....:.||
  Rat   407 --------EPEDLAQLAPTPGDAIISLDFGSQN------------------FDESSTYGKAILPP 445

  Fly   538 --------LNDLVVSSSSSVGGGTASSAG--GGSSASASSSGVYSTEMQYPDTTTGNLYYNN-NN 591
                    .:....|.|.|:...|...||  |.::.||:||...||.....|      ||:: .|
  Rat   446 GQPWATELRSHSAQSESRSLPAFTVPQAGSPGNATPSATSSSSCSTPSSPED------YYSSLEN 504

  Fly   592 HYYYDYDATVDVATSMIRPFSANSNSCSSSSE-SERQLST------------------GNASIVN 637
            |      ..::|...:....:...:.||:.:: :|..|.|                  ....:|.
  Rat   505 H------LKIEVIEKLFAMDTEAKDQCSTQTDFNELDLETLAPYIPMDGEDFQLSPICPEEPLVP 563

  Fly   638 ETSPS---QTTYSDLSHNFEL--------SYFSDNSSQQ-HQHQQQQQH 674
            | ||.   |..:|.:|..|:.        ::|.|...|| ...:.:.:|
  Rat   564 E-SPQPNPQHCFSTMSSIFQPLTPGASQGTFFLDKYPQQLESRKTESEH 611

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sim	NP_524340.2	HLH	26..78	CDD:238036	29/51 (57%)
		PAS	102..215	CDD:279347	37/112 (33%)
		PAS	102..163	CDD:214512	26/60 (43%)
		PAS	278..376	CDD:238075	38/97 (39%)
		PAS_3	291..377	CDD:285623	33/85 (39%)
Epas1	XP_038967774.1	bHLH-PAS_HIF2a_PASD2	20..85	CDD:381571	29/57 (51%)
		PAS	97..158	CDD:214512	26/60 (43%)
		PAS_3	265..352	CDD:369881	33/86 (38%)
		HIF-1	527..559	CDD:402842	3/31 (10%)
		HIF-1a_CTAD	849..884	CDD:400914
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000232
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.910