DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsc70-2 and HSPA5

DIOPT Version :9

Sequence 1:	NP_524339.1	Gene:	Hsc70-2 / 41609	FlyBaseID:	FBgn0001217	Length:	633	Species:	Drosophila melanogaster
Sequence 2:	NP_005338.1	Gene:	HSPA5 / 3309	HGNCID:	5238	Length:	654	Species:	Homo sapiens

Alignment Length:	628	Identity:	367/628 - (58%)
Similarity:	478/628 - (76%)	Gaps:	16/628 - (2%)

- Green bases have known domain annotations that are detailed below.

  Fly     7 IGIDLGTTYSCVGVWQNSKVEIIANDQGNRTTPSYVAFN-ETERLIGDPAKNQVAMNAKNTVFDA 70
            :|||||||||||||::|.:|||||||||||.|||||||. |.||||||.||||:..|.:||||||
Human    31 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 95

  Fly    71 KRLIGRKFDDKKIQEDLKLWPFKVINEKGKPKIEVEF-KGERKRFAPEEISSMVLTKMREIAEVY 134
            ||||||.::|..:|:|:|..||||:.:|.||.|:|:. .|:.|.|||||||:||||||:|.||.|
Human    96 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 160

  Fly   135 LGGKVKDAVVTVPAYFNDSQRQATKDAGSIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 199
            ||.||..|||||||||||:|||||||||:||||||:||||||||||:||||||. :||:|:|:||
Human   161 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKR-EGEKNILVFD 224

  Fly   200 LGGGTFDVSVLSIDEGSLFEVKATAGDTHLGGEDFDNRLVNHFAEEFKRKHKSDIKGNARALRRL 264
            |||||||||:|:||.| :|||.||.|||||||||||.|::.||.:.:|:|...|::.:.||:::|
Human   225 LGGGTFDVSLLTIDNG-VFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKL 288

  Fly   265 RTACERAKRTLSSSTEASLEIDALHEGIDFYSKISRARFEELNMDLFRSTMQPVERALSDAKMDK 329
            |...|:|||.|||..:|.:||::.:||.||...::||:|||||||||||||:||::.|.|:.:.|
Human   289 RREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKK 353

  Fly   330 KAIHDVVLVGGSTRIPKIQKLLQDLFGGKQLNLSINPDEAVAYGAAVQAAILTGVGSSQIQDLLL 394
            ..|.::|||||||||||||:|:::.|.||:.:..|||||||||||||||.:|:  |.....||:|
Human   354 SDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLS--GDQDTGDLVL 416

  Fly   395 VDVAPLSLGIETAGGVMTNLVERNARIPCKQQQIFTTYSDNQNAVTIQVYEGERAMTKDNNLLGT 459
            :||.||:|||||.|||||.|:.||..:|.|:.|||:|.||||..|||:||||||.:||||:||||
Human   417 LDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGT 481

  Fly   460 FNLTGIPPAPRGVPQIEVAFDLNADGILNVNAKDNSTGKSEKITISNDKGRLSKAEIDRMLSEAE 524
            |:||||||||||||||||.|:::.:|||.|.|:|..||...||||:||:.||:..||:||:::||
Human   482 FDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAE 546

  Fly   525 KYKVEDDRQRERVQSKNNLEAYIYACRQAVDDAP--SGVLSETERSKVRDKCSSEASWLDKNSLA 587
            |:..||.:.:||:.::|.||:|.|:.:..:.|..  .|.||..::..:......:..||:.:..|
Human   547 KFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDA 611

  Fly   588 EKEEFESHLKECQRICTPIMSKIHGGKKGKSSMGKGSHPTVEE 630
            :.|:|::..||.:.|..||:||::|        ..|..||.||
Human   612 DIEDFKAKKKELEEIVQPIISKLYG--------SAGPPPTGEE 646

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsc70-2	NP_524339.1	PTZ00009	1..632	CDD:240227	367/628 (58%)
Hsc70-2	NP_524339.1	HSPA1-2_6-8-like_NBD	6..382	CDD:212675	246/376 (65%)
HSPA5	NP_005338.1	Required for interaction with KIAA1324. /evidence=ECO:0000269\|PubMed:26045166	1..80		39/48 (81%)
		HSP70	30..635	CDD:394970	361/607 (59%)
		Nucleotide-binding (NBD). /evidence=ECO:0000303\|PubMed:28286085	125..280		110/156 (71%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	409..419		3/9 (33%)
		Substrate-binding (SBD). /evidence=ECO:0000303\|PubMed:28286085	420..500		58/79 (73%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	633..654		7/22 (32%)
		Prevents secretion from ER. /evidence=ECO:0000255	651..654
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	1	1.010	-	-		D288077at2759
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	7	6.730