DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-theta and Polq

DIOPT Version :9

Sequence 1:	NP_524333.1	Gene:	DNApol-theta / 41571	FlyBaseID:	FBgn0002905	Length:	2059	Species:	Drosophila melanogaster
Sequence 2:	NP_084253.1	Gene:	Polq / 77782	MGIID:	2155399	Length:	2544	Species:	Mus musculus

Alignment Length:	2576	Identity:	736/2576 - (28%)
Similarity:	1103/2576 - (42%)	Gaps:	767/2576 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   172 QNLSSMSPNQLRVSPNSSRIREAMPERPAMPLDLNTLRSISAWNLPMSIQAEYKKKGVVDMFDWQ 236
            :.|:....|:.|||  ..:|.:.:               ::.|.||.::..:|...||..||:||
Mouse    48 RRLTGTGTNKRRVS--DDQIDQLL---------------LANWGLPKAVLEKYHSFGVRKMFEWQ 95

  Fly   237 VECLSKPRLLFEHCNLVYSAPTSAGKTLVSEILMLKTVLERGKKVLLILPFISVVREKMFYMQDL 301
            .|||....:| |..|||||||||||||||:|:|:||.|||..||.|.||||:||.:||..|:|.|
Mouse    96 AECLLLGHVL-EGKNLVYSAPTSAGKTLVAELLILKRVLETRKKALFILPFVSVAKEKKCYLQSL 159

  Fly   302 LTPAGYRVEGFYGGYTPPGGFESLHVAICTIEKANSIVNKLMEQGKLETIGMVVVDEVHLISDKG 366
            ....|.:|:|:.|..:|.|.|.||.:|:||||:||.:||:|:|:.|::.:|||||||:|::.|..
Mouse   160 FQEVGLKVDGYMGSTSPTGQFSSLDIAVCTIERANGLVNRLIEENKMDLLGMVVVDELHMLGDSH 224

  Fly   367 RGYILELLLAKILYMSRRNGLQ-----------IQVITMSATLENVQLLQSWLDAELYITNYRPV 420
            |||:|||||.||.|::|::...           :|::.|||||.|:||:.|||:||||.|::|||
Mouse   225 RGYLLELLLTKICYVTRKSASHQAESASTLSNAVQIVGMSATLPNLQLVASWLNAELYHTDFRPV 289

  Fly   421 ALKEMIKVGTVIYDHRLKLVRDVAKQKVL-LKGLENDSDDVALLCIETLLEGCSVIVFCPSKDWC 484
            .|.|.||:|..|||..:||||:.  |.:| :||   |.|.:..||.||:.:..||::|||||.||
Mouse   290 PLLESIKIGNSIYDSSMKLVREF--QPLLQVKG---DEDHIVSLCYETIQDNHSVLIFCPSKKWC 349

  Fly   485 ENLAVQLATA---IHVQ----IKSE----TVLGQRLRTNLNPRAIAEVKQQLRDIPTGLDGVMSK 538
            |.:|..:|..   :|.|    :||.    .:|.|        :::.||..||:..|:|||.|:..
Mouse   350 EKVADIIAREFYNLHHQPEGLVKSSEFPPVILDQ--------KSLLEVMDQLKRSPSGLDSVLKN 406

  Fly   539 AITYACAFHHAGLTTEERDIIEASFKAGALKVLVATSTLSSGVNLPARRVLIRSPLFGGKQMSSL 603
            .:.:..||||||||.|||||||.:|:.|.::||.||||||||||||||||:||:|:|.|:.:..|
Mouse   407 TVPWGVAFHHAGLTFEERDIIEGAFRQGFIRVLAATSTLSSGVNLPARRVIIRTPIFSGQPLDIL 471

  Fly   604 TYRQMIGRAGRMGKDTLGESILICNEINARMGRDLVVSELQPITSCLDMDG--STHLKRALLEVI 666
            ||:||:|||||.|.||:|||||:|.......|..|:...|:|:.|||...|  :..:.||:||:|
Mouse   472 TYKQMVGRAGRKGVDTMGESILVCKNSEKSKGIALLQGSLEPVHSCLQRQGEVTASMIRAILEII 536

  Fly   667 SSGVANTKEDIDFFVNCTLLSA--QKAFHAKEKPPDEESDANYINDALDFLVEYEFVRL-QRNEE 728
            ..|||:|.:|:..:..||.|:|  |:.....::..| ::....|:..:.:|:|.||::: :..:.
Mouse   537 VGGVASTSQDMQTYAACTFLAAAIQEGKQGMQRNQD-DAQLGAIDACVTWLLENEFIQVAEPGDG 600

  Fly   729 RETAVYVATRLGAACLASSMPPTDGLILFAELQKSRRSFVLESELHAVYLVTPYSVCYQLQDIDW 793
            ....||..|.||:|.|:||:.|||.|.:||:||::.:.||||::||.||||||  |......|||
Mouse   601 TGGKVYHPTHLGSATLSSSLSPTDTLDIFADLQRAMKGFVLENDLHIVYLVTP--VFEDWISIDW 663

  Fly   794 LLYVHMWEKLSSPMKKVGELVGVRDAFLYKALRG----QTKLDYKQMQIHKRFYIALALEELVNE 854
            ..:..:||||.:.||:|.|||||.:.||.:.::|    :|:..::||.|||||:.:|.|.:|::|
Mouse   664 YRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISE 728

  Fly   855 TPINVVVHKYKCHRGMLQSLQQMASTFAGIVTAFCNSLQWSTLALIVSQFKDRLFFGIHRDLIDL 919
            .|:..:..||.|:||.:|||||.|:.:||::|.|.|.|.|..:.|::|||:.||.|||.|:|.||
Mouse   729 IPLKDINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDL 793

  Fly   920 MRIPDLSQKRARALFDAGITSLVELAGADPVELEKVLYNSISFDSAKQHDHENADEAAKRNVVRN 984
            :|:..|:.:|||.|:.:|..::.:||.||..|:|..|.||:.|.||::...|..:.|.:|..:|.
Mouse   794 IRVSLLNAQRARFLYASGFLTVADLARADSAEVEVALKNSLPFKSARKAVDEEEEAAEERRSMRT 858

  Fly   985 FYITGKAGMTVSEAAKLLIGEARQFVQHEIGLGTIKW-------------------------TQT 1024
            .::||| |::..|||.|::.||:..:|.::....::|                         :||
Mouse   859 IWVTGK-GLSAREAAALIVEEAKMILQQDLIEMGVRWDPKSPLSSSTHSRTSTSEVKEHTFKSQT 922

  Fly  1025 QAG----VEIASRAIHDGGEVD------------------------------------------- 1042
            ::.    ..:...:|...|..|
Mouse   923 KSSHKRLASMGRNSIRASGSNDKPSPDAERGIDDCSEHADSLCKFQGNFEPQTPSICTARKRTSL 987

  Fly  1043 ------LHMSLEEEQPPVKRKL----SIEENGTANSQKNPRLETVVDTQRGYKVDK--------- 1088
                  |..||:|.:|..|..|    |.:...||.|..:.::...:.:.|..|..|
Mouse   988 GINKEMLRKSLKEGKPSTKEVLQTFSSEKTRKTALSFSSEQVNNTLPSGRDRKYQKKSWGSSPVR 1052

  Fly  1089 ---------------------------------------------NIANQSKMNPNLKEIDAQNK 1108
                                                         :.|.:....|:| .:..|..
Mouse  1053 DSGMHRGDLQGQTMCTSALCEDSQKSLEEQNAEFRSPGLFAKHLPSCAKEKCKKPSL-PLQRQQA 1116

  Fly  1109 ARRNST------------------------------------------AHMDNLNPI-------- 1123
            ..|.||                                          :.:.:..|:
Mouse  1117 CSRRSTESCAAVGHPAAGSSPAAARDRRGLAARETEKGNEALTENGGESQLQDTYPVSQYLEYHS 1181

  Fly  1124 --------------------------SND-------------------PCQNNVNVKTAQ-PIIS 1142
                                      |||                   ||:..:...|.: ..:.
Mouse  1182 EKHTNTCTRQKTLTEGQAGSSYVARDSNDAAPIKCERMKLNSKDRDSNPCRQALGSYTGRTEALQ 1246

  Fly  1143 NLNDIQKQGSQIEKM----------------------KINPATVVCS---------------PQL 1170
            :...:.:.|.|.|.:                      ..|||  .|.               .|:
Mouse  1247 STAKLGQAGGQCENLLNSSGVQGKTGAHATNRTEHSHASNPA--FCDFGDSLDLDTQSEEIIEQM 1309

  Fly  1171 ANEEKPSTSQSARRKLVNE-------------------GMAERRRVALMKIQQ------------ 1204
            |.|   :|.|.|:..::.|                   |.|....|...|::.
Mouse  1310 ATE---NTMQGAKAVVIMEEGSAMQNKCHSTPGDQHVPGAANTDHVDSKKVESVKANTEKNINRG 1371

  Fly  1205 -------RTQKEN----QSKDQPIQASRSNQL---------------SSPVNRTP---------- 1233
                   .||.||    :..:..:..|:.|..               .:|..|||
Mouse  1372 APVSLIFHTQGENGACFKGNEHSVTDSQLNSFLQGFETQEIVKPIIPLAPQMRTPTGVEEESLPE 1436

  Fly  1234 ----------------------------ANRWTQSENPNNEMNNSQLPRRNPRNQSPVPNANRTA 1270
                                        ||:...||...|..:|...|:.:|.....|.....|.
Mouse  1437 TSLNMSDSILFDSFGEDGFGQGQSPDIKANQPLLSEMTPNHFSNPPHPQEDPVMTPTVSEPQGTQ 1501

  Fly  1271 SRKVSNAEEDLFMAD-DSFML----------------NTGLAAALTAAESKIA--SCTEADVIPS 1316
            .:.|..:.|.:..:| ||..:                |:.....|..::|.:.  .|.:..::..
Mouse  1502 QQGVCLSGESIIFSDIDSAQVIEALDNMAAFHVQENCNSVALKTLEPSDSAVLGNECPQGKLVRG 1566

  Fly  1317 SQ------PKEPEV--------IGA---LTPHASRLKRSDQLRSQR------------------- 1345
            .|      ||..|.        .||   |:|...|:  .|::.|.|
Mouse  1567 DQNEGSPKPKLTETNQDNSFTWSGASFNLSPELQRI--LDKVSSPRENEKPKMIHVNLSSFEGNS 1629

  Fly  1346 ---------------IQSPSPTPQREIE---IDLESKNESNGVSS---MEISDMSMENPLM-KNP 1388
                           :|..|..|..|::   ..||||....|.||   .:.|..:.:|.|: ..|
Mouse  1630 KESHEREEINSDLGTVQRTSVFPSNEVKNRTEGLESKARHGGASSPLPRKESAAADDNGLIPPTP 1694

  Fly  1389 LHLNASHIM----------------------SC--------------------------SKVD-- 1403
            :..:||.:.                      ||                          |..|
Mouse  1695 VPASASKVAFPEILGTSVKRQKASSALQPGESCLFGSPSDNQNQDLSQELRDSLKDYDGSVADTS 1759

  Fly  1404 ------------ETASSFSSIDIIDVCGHRNAFQAAIIEINNATRLGFSVG---LQAQAGKQKPL 1453
                        :.:.|..|:.||||...:..||..:.|.....|...|:.   :.:....:...
Mouse  1760 FFLQSQDGLLLTQASCSSESLAIIDVASDQILFQTFVKEWQCQKRFSISLACEKMTSSMSSKTAT 1824

  Fly  1454 IGSNLLINQVAAAENREAAARERVLFQVDDTNF---------ISGVSFCLADNVAYYWNMQIDER 1509
            ||..|  .||:..:  ||.        |:|..|         :.|::.|.....|||.::|.:::
Mouse  1825 IGGKL--KQVSLPQ--EAT--------VEDAGFPVRGCDGAVVVGLAVCWGAKDAYYLSLQKEQK 1877

  Fly  1510 AAYQG---VPTPLKVQELCNLMARKDLTLVMHDGKEQLKMLRKAIPQLK---------------- 1555
            .:...   .|.||            |.||.:   ||:::.|:..:.:..
Mouse  1878 QSEISPSLAPPPL------------DATLTV---KERMECLQSCLQKKSDRERSVVTYDFIQTYK 1927

  Fly  1556 --------RISAKLEDAKVANWLLQPD-KTVNFLNMCQTFAPECTGLANLCGSGRGYSSYGLDTS 1611
                    .:....||.|||.|||.|| |.....::..:|.|....|.....:|.|..|.||:.:
Mouse  1928 VLLLSCGISLEPSYEDPKVACWLLDPDSKEPTLHSIVTSFLPHELALLEGMETGPGIQSLGLNVN 1992

  Fly  1612 SAILPRIRTAIESCVTLHILQG-----QTENLSRIGNGDLLKFFHDIEMPIQLTLCQMELVGFPA 1671
            :....|.|.::||.:..:.:..     |.|||..|        |..:|||.|..|..:||.|...
Mouse  1993 TEHSGRYRASVESVLIFNSMNQLNSLLQKENLHDI--------FCKVEMPSQYCLALLELNGIGF 2049

  Fly  1672 QKQRLQQLYQRMVAVMKKVETKIYEQHGSRFNLGSSQAVAKVLGLHRK----------------- 1719
            .....:.....|.|.:..:||:.|:..|..|:..|:..:|:||.|..|
Mouse  2050 STAECESQKHVMQAKLDAIETQAYQLAGHSFSFTSADDIAQVLFLELKLPPNGEMKTQGSKKTLG 2114

  Fly  1720 ------AKGR-------VTTSRQVLEKLNS--PISHLILGYRKLSGLLAKSIQPLME------CC 1763
                  ..||       .:||:.:|.||..  |:..|||.:|::|..:.|.:.||..      ..
Mouse  2115 STRRGNESGRRMRLGRQFSTSKDILNKLKGLHPLPGLILEWRRISNAITKVVFPLQREKHLNPLL 2179

  Fly  1764 QADRIHGQSITYTATGRISMTEPNLQNVAKEFSIQ---------------------VGSDV---- 1803
            :.:||:..|.::||||||:.||||:|||.::|.|:                     :|.|.
Mouse  2180 RMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVRESPPSQAPKGRFPMAIGQDKKVYG 2244

  Fly  1804 VH-------------------ISCRSPFMPTDESRCLLSADFCQLEMRILAHMSQDKALLEVMKS 1849
            :|                   :|.|..|:|. ....:|:||:.|||:|||||:|:|..|::|:.:
Mouse  2245 LHPGHRTQMEEKASDRGVPFSVSMRHAFVPF-PGGLILAADYSQLELRILAHLSRDCRLIQVLNT 2308

  Fly  1850 SQDLFIAIAAHWNKIEESEVTQDLRNSTKQVCYGIVYGMGMRSLAESLNCSEQEARMISDQFHQA 1914
            ..|:|.:|||.|..||...|..|||...||:||||:||||.:||.|.:...|.:|....|.|...
Mouse  2309 GADVFRSIAAEWKMIEPDAVGDDLRQHAKQICYGIIYGMGAKSLGEQMGIKENDAASYIDSFKSR 2373

  Fly  1915 YKGIRDYTTRVVNFARSKGFVETITGRRRYLENINSDVEHLKNQAERQAVNSTIQGSAADIAKNA 1979
            ||||..:....|...|..||||||.||||||..|..|..:.|..|||||:|:|:|||||||.|.|
Mouse  2374 YKGINHFMRDTVKNCRKNGFVETILGRRRYLPGIKDDNPYHKAHAERQAINTTVQGSAADIVKIA 2438

  Fly  1980 ILKMEKNIERY---------REKLALGDNSVDL-----------------VMHLHDELIFEVPTG 2018
            .:.::|.:|.:         ||.:...|.:..|                 ::.|||||::||...
Mouse  2439 TVNIQKQLETFRSTFKSHGHRESMLQNDRTGLLPKRKLKGMFCPMRGGFFILQLHDELLYEVAEE 2503

  Fly  2019 KAKKIAKVLSLTMENCVKLSVPLKVKLRIGRSWGEFKEVSV 2059
            ...::|:::...||..:||||.||||::||.||||.|:..|
Mouse  2504 DVVQVAQIVKNEMECAIKLSVKLKVKVKIGASWGELKDFDV 2544

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-theta	NP_524333.1	BRR2	209..978	CDD:224125	363/800 (45%)
		DEAD	251..404	CDD:278688	86/163 (53%)
		HELICc	542..616	CDD:197757	50/73 (68%)
		PolA	1489..2055	CDD:223820	217/706 (31%)
		DNA_pol_A_theta	1668..2053	CDD:176475	164/492 (33%)
Polq	NP_084253.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..57		1/8 (13%)
		PRK02362	75..830	CDD:235032	354/771 (46%)
		DEAD	95..268	CDD:278688	89/173 (51%)
		DEAH box. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00541	215..218		2/2 (100%)
		HELICc	411..484	CDD:197757	50/72 (69%)
		Interaction with RAD51. /evidence=ECO:0000250\|UniProtKB:O75417	844..890		15/46 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	896..955		5/58 (9%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1022..1058		4/35 (11%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1128..1167		0/38 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1266..1288		1/21 (5%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1331..1353		2/21 (10%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1478..1501		4/22 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1557..1591		5/33 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1606..1697		17/90 (19%)
		PolA	1852..2540	CDD:223820	217/711 (31%)
		DnaQ_like_exo	1853..2040	CDD:299142	48/209 (23%)
		DNA_pol_A_theta	2046..2539	CDD:176475	165/493 (33%)
		Loop 1. /evidence=ECO:0000250\|UniProtKB:O75417	2097..2132		3/34 (9%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2104..2124		0/19 (0%)
		Loop 2. /evidence=ECO:0000250\|UniProtKB:O75417	2212..2276		8/64 (13%)
		Loop 3. /evidence=ECO:0000250\|UniProtKB:O75417	2445..2489		5/43 (12%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837075
Domainoid	1	1.000	359	1.000	Domainoid score	I959
eggNOG	1	0.900	-	-		E1_COG0749
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H32727
Inparanoid	1	1.050	994	1.000	Inparanoid score	I305
Isobase	1	0.950	-	0	Normalized mean entropy	S5818
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D103838at33208
OrthoFinder	1	1.000	-	-		FOG0001214
OrthoInspector	1	1.000	-	-		oto95181
orthoMCL	1	0.900	-	-		OOG6_101025
Panther	1	1.100	-	-	O	PTHR10133
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2964
SonicParanoid	1	1.000	-	-		X4573
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	16	15.740