Protein Alignment ATP8B and DNF1

DIOPT Version :9

• Sequence 1: NP_731669.2
• Gene: ATP8B / 41469
• FlyBaseID: FBgn0037989
• Length: 1726
• Species: Drosophila melanogaster
• Sequence 2: NP_011093.3
• Gene: DNF1 / 856913
• SGDID: S000000968
• Length: 1571
• Species: Saccharomyces cerevisiae
• Alignment Length: 1879
• Identity: 518/1879 - (27%)
• Similarity: 773/1879 - (41%)
• Gaps: 579/1879 - (30%)

  Fly    30 PIETELQ-EDNERTPTKGAGNVDEPGIEAPEASCSAPSKRTRWFQFARSSKNRRKRLHCNED--- 90
            |.|...| |||                .:.|.:..||   |.:...|.|:|..|.::..|::
Yeast    15 PFEDTFQFEDN----------------SSNEDTHIAP---THFDDGATSNKYSRPQVSFNDETPK 60

  Fly    91 ---EDVKQ------PERRNSDLEGSPRACLAQPEGMNIGSST-------QTIATPLMIGDSFYSL 139
               ||.::      .|..|...:.:|:        :|.||.|       .....|....|.....
Yeast    61 NKREDAEEFTFNDDTEYDNHSFQPTPK--------LNNGSGTFDDVELDNDSGEPHTNYDGMKRF 117

  Fly   140 APG-----GGNPPPNKSDDQELYKQRDPS----VTSESKSVPSLRRLSQIRRRRSSYYFSENERR 195
            ..|     .|||...:|...:..::..|:    .|.:.....::.|..::   |:.||.....:.
Yeast   118 RMGTKRNKKGNPIMGRSKTLKWARKNIPNPFEDFTKDDIDPGAINRAQEL---RTVYYNMPLPKD 179

  Fly   196 IRANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVT 260
            :  .|:|.|...:|..|.|:|:||:..||||.|:|.||...||.|||.|::|..........|..
Yeast   180 M--IDEEGNPIMQYPRNKIRTTKYTPLTFLPKNILFQFHNFANVYFLVLIILGAFQIFGVTNPGL 242

  Fly   261 TAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTLR---------------------NGKLV 304
            :|:||:.::.:||:|||.:|.:|.:.|.:|||.|:..|.                     |.:|:
Yeast   243 SAVPLVVIVIITAIKDAIEDSRRTVLDLEVNNTKTHILEGVENENVSTDNISLWRRFKKANSRLL 307

  Fly   305 -------------EAK------------------------------------------------- 307
                         |.|
Yeast   308 FKFIQYCKEHLTEEGKKKRMQRKRHELRVQKTVGTSGPRSSLDSIDSYRVSADYGRPSLDYDNLE 372

  Fly   308 -------------------------WSEVQVGDVIRLDNNQFVAADTLLLSTSEPNGLCFIETAE 347
                                     |..|:|||::|:.||..:.||.:|||||:.:|.|::||..
Yeast   373 QGAGEANIVDRSLPPRTDCKFAKNYWKGVKVGDIVRIHNNDEIPADIILLSTSDTDGACYVETKN 437

  Fly   348 LDGETNLKAKQCL--TETIELGD--RHDSLWNFNGEIICERPNNLLNKFDGTLIW--------RG 400
            ||||||||.:|.|  |.||....  .....|     |..|.|::.|..:.|.:.|        |.
Yeast   438 LDGETNLKVRQSLKCTNTIRTSKDIARTKFW-----IESEGPHSNLYTYQGNMKWRNLADGEIRN 497

  Fly   401 QRFALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKTQFKSTGVDRLLNF-IIIGIVLF 464
            :...::|  :|||||.||||:|..|||:|.|.|||:|.|||.|..|.:.:.|.||| ::|..||.
Yeast   498 EPITINN--VLLRGCTLRNTKWAMGVVMFTGGDTKIMLNSGITPTKKSRISRELNFSVVINFVLL 560

  Fly   465 LVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDY--IPTGATVIGLLVFFSYAIVLNTVVPI 527
            .: :|.:..|...::....|:....|         ::  |...|...|.:.|:...|:..::|||
Yeast   561 FI-LCFVSGIANGVYYDKKGRSRFSY---------EFGTIAGSAATNGFVSFWVAVILYQSLVPI 615

  Fly   528 SLYVSVEVIRFVQSFLINWDEEMY-----YPTTNTYAKARTTTLNEELGQIQYIFSDKTGTLTQN 587
            |||:|||:|:..|:..|..|..:|     ||.|     .::..::::|||::|||||||||||||
Yeast   616 SLYISVEIIKTAQAAFIYGDVLLYNAKLDYPCT-----PKSWNISDDLGQVEYIFSDKTGTLTQN 675

  Fly   588 IMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNSNTNNRPSPLSGAIVAPPAAPPPIILVH 652
            :|.|.||:|||.|||...   |..|..:.::|.|  :..|..|.                    .
Yeast   676 VMEFKKCTINGVSYGRAY---TEALAGLRKRQGI--DVETEGRR--------------------E 715

  Fly   653 KAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMDASEKRPGLKHVRYSAPSRSQDADAGRL 717
            |||:...:.:|:
Yeast   716 KAEIAKDRDTMI----------------------------------------------------- 727

  Fly   718 SPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLSRTSSCDKVKILHDSQQTETHNMHHSSH 782
                           :|.|.:||.
Yeast   728 ---------------DELRALSGN----------------------------------------- 736

  Fly   783 NRKRLVKIRFKKAPSTATLGVVISQLEHHLDEEPQSTSTLSTTKHHHHRSKAFATNWSSSPHRVH 847
                                   ||.      .|:..:.:         ||.|..:...:...|.
Yeast   737 -----------------------SQF------YPEEVTFV---------SKEFVRDLKGASGEVQ 763

  Fly   848 ALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETVDG---KLEYQAQ 909
                                .|..        ||   |...|||||:|:.|....   ||:.:||
Yeast   764 --------------------QRCC--------EH---FMLALALCHSVLVEANPDNPKKLDLKAQ 797

  Fly   910 SPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYELLNILDFNNVRKRMSVI-----LRRGD 969
            ||||||||:.||:.||.|..:|...:.||:.|..:|:|:||||:||:.|||||.|     |..||
Yeast   798 SPDEAALVATARDVGFSFVGKTKKGLIIEMQGIQKEFEILNILEFNSSRKRMSCIVKIPGLNPGD 862

  Fly   970 S--MVLYCKGADNVIYDRLH----GGQEDLKARTQDHLNKFAGEGLRTLALAERRLTEQYYNDWR 1028
            .  .:|.|||||::||.||.    ...|.:..:|..||.::|.||||||.:|:|.|:...|..|.
Yeast   863 EPRALLICKGADSIIYSRLSRQSGSNSEAILEKTALHLEQYATEGLRTLCIAQRELSWSEYEKWN 927

  Fly  1029 SRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDK 1093
            .:...||.|:.:||.:|..:.:.||.|:.|:|.|||||:||||||..|..|..||||:|||||||
Yeast   928 EKYDIAAASLANREDELEVVADSIERELILLGGTAIEDRLQDGVPDCIELLAEAGIKLWVLTGDK 992

  Fly  1094 QETAINIGYSCQLLTDELADVFI-VDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDP---FDRLN 1154
            .|||||||:||.||.:|:..:.| ..|:.|:|.                    |.:|   .|.|.
Yeast   993 VETAINIGFSCNLLNNEMELLVIKTTGDDVKEF--------------------GSEPSEIVDALL 1037

  Fly  1155 SDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRWDEKNKDNKGGPDSAECNNLFGDEK 1219
            |....:..::|.::...|                        |..||::             ..|
Yeast  1038 SKYLKEYFNLTGSEEEIF------------------------EAKKDHE-------------FPK 1065

  Fly  1220 GSEDGGTASIVVDENTGFALVVNGHSLVHCL-SPELENKFLDIASQCKAVICCRVTPLQKALVVE 1283
            |:               :|:|::|.:|...| ..::..|||.:...|:||:||||:|.|||.||:
Yeast  1066 GN---------------YAIVIDGDALKLALYGEDIRRKFLLLCKNCRAVLCCRVSPSQKAAVVK 1115

  Fly  1284 LIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWS 1348
            |:|.:.:.:|||||||:|||:||::|.:|:||:|:||.|||:.|||:|.|||||.||:|||||||
Yeast  1116 LVKDSLDVMTLAIGDGSNDVAMIQSADVGIGIAGEEGRQAVMCSDYAIGQFRYLARLVLVHGRWS 1180

  Fly  1349 YYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVS 1413
            |.|:.:.:..|||||..|.|...||.::..|....:::..::..|||.:|||||:.||:.:|||:
Yeast  1181 YKRLAEMIPEFFYKNMIFALALFWYGIYNDFDGSYLYEYTYMMFYNLAFTSLPVIFLGILDQDVN 1245

  Fly  1414 DKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLIPYGVYKDG--VSANGFVVSDHMT 1476
            |..||..|:||..|:..:.:|.|:|::.:|.|.:.|::.|..||.||...  |::||..:.....
Yeast  1246 DTISLVVPQLYRVGILRKEWNQRKFLWYMLDGLYQSIICFFFPYLVYHKNMIVTSNGLGLDHRYF 1310

  Fly  1477 LGAVVATILIVDNTAQISLYTSYW-------------TVVNHVTIWGSLVWYFVLDYFYN---YV 1525
            :|..|.||.::.....:.|:...|             .|.....||.|.:   ....|:.   .:
Yeast  1311 VGVYVTTIAVISCNTYVLLHQYRWDWFSGLFIALSCLVVFAWTGIWSSAI---ASREFFKAAARI 1372

  Fly  1526 IGGPYVGSLTQAMKDLTFWVTMLITVMALVAPVLAYK-----FYLLDVHPSLSDKIRQKSLKKIH 1585
            .|.|            :||....:.|:..:.|...|.     ||..||      :|.::..:..|
Yeast  1373 YGAP------------SFWAVFFVAVLFCLLPRFTYDSFQKFFYPTDV------EIVREMWQHGH 1419

  Fly  1586 ------SRASSDVRRTASSRRGR--RSVRSGYAFAHQEGFGRLITSGKIMHKLPQDF-----AFP 1637
                  ....:|..|...::.|:  ..:..|.|.:...|.........:..::|..|     ..|
Yeast  1420 FDHYPPGYDPTDPNRPKVTKAGQHGEKIIEGIALSDNLGGSNYSRDSVVTEEIPMTFMHGEDGSP 1484

  Fly  1638 LG--------LGTKKTQVLHNNLNSADGPNSKGNNVTGHHMVNNNTNMRHNQNQNHSSM 1688
            .|        ...|:||.|      ...|..:.....|.   ..:||:|.:.::....|
Yeast  1485 SGYQKQETWMTSPKETQDL------LQSPQFQQAQTFGR---GPSTNVRSSLDRTREQM 1534

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ATP8B	NP_731669.2	PhoLip_ATPase_N	195..260	CDD:292826	25/64 (39%)
		Cation_ATPase	891..985	CDD:289987	51/103 (50%)
		HAD_like	<1261..1324	CDD:304363	35/62 (56%)
		PhoLip_ATPase_C	1324..1571	CDD:292829	87/269 (32%)
DNF1	NP_011093.3	ATPase-Plipid	191..1413	CDD:273734	457/1539 (30%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	151	1.000	Domainoid score	I929
eggNOG	1	0.900	-	-		E2759_KOG0206
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000076
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100089
Panther	1	1.100	-	-	LDO	PTHR24092
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.810