Protein Alignment ATP8B and DNF3

DIOPT Version :9

• Sequence 1: NP_731669.2
• Gene: ATP8B / 41469
• FlyBaseID: FBgn0037989
• Length: 1726
• Species: Drosophila melanogaster
• Sequence 2: NP_013885.1
• Gene: DNF3 / 855197
• SGDID: S000004772
• Length: 1656
• Species: Saccharomyces cerevisiae
• Alignment Length: 1982
• Identity: 512/1982 - (25%)
• Similarity: 788/1982 - (39%)
• Gaps: 694/1982 - (35%)

  Fly    15 RRRPDTLELSVL---------GGQPIETELQEDNERTPTKGAGNVDEPGIEAPEASCSAPSKRTR 70
            |||..:|...:.         |....|.||::.||.....|:.|.|:...:....:.:|
Yeast     8 RRRSSSLRTQMFNKHLYDKYRGRTDDEIELEDINESKTFSGSDNNDKDDRDETSGNYAA------ 66

  Fly    71 WFQFARSSKNRRKRLHCNEDEDVKQPERRNSDLEGSPRACLAQPEGMNIGSSTQTIATPLMIGDS 135
                               :||.:..|      .|||                          |.
Yeast    67 -------------------EEDYEMEE------YGSP--------------------------DV 80

  Fly   136 FYSLAPGGGNPPPNKSDDQELYKQRDPSVTSESKS---VPSLRRLSQIRRRRSSYYFSENERRIR 197
            .||:.        .|..|..|.::|    |..||.   :|.:...:.|..::::     .:|...
Yeast    81 SYSII--------TKILDTILDRRR----TFHSKDGRHIPIILDHNAIEYKQAA-----TKRDGH 128

  Fly   198 ANDKEFNAQFKYHNNYIKTSKYSLFTFLPFNLLEQFQRLANFYFLCLLVLQLIPAISSLTPVTTA 262
            ..|:.||.  .|.:|.|.:|:|:.::|||..|..||.:|||.||..:.|||:||..|:....||.
Yeast   129 LIDERFNK--PYCDNRITSSRYTFYSFLPRQLYAQFSKLANTYFFIVAVLQMIPGWSTTGTYTTI 191

  Fly   263 IPLIGVLTLTAVKDAYDDIQRHISDSQVNNR-------------------------------KS- 295
            |||...:.::..::|:||.:||..|.:.||:                               ||
Yeast   192 IPLCVFMGISMTREAWDDFRRHRLDKEENNKPVGVLVKDGNNDAQEVYTLPSSVVSSTAYLTKSA 256

  Fly   296 ------------------------------KTLRNGKLVEAKWSEVQVGDVIRLDNNQFVAADTL 330
                                          |...|..:.:.||.:::|||.:.|..:.:|.||.|
Yeast   257 AAENNPPLNDDRNSSQGHFLDTHFNNFELLKNKYNVHIHQKKWEKLRVGDFVLLTQDDWVPADLL 321

  Fly   331 LLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHDSLWNFNGEIICERPNNLLNKFDGT 395
            ||:....|..||:||..||||||||:||...|..:|......|.|.|.::..|.||..|..|:|.
Yeast   322 LLTCDGENSECFVETMALDGETNLKSKQPHPELNKLTKAASGLANINAQVTVEDPNIDLYNFEGN 386

  Fly   396 LIWRGQR------FALDNEKILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGKT-QFKSTGVDRL 453
            |..:..|      :.|..:.::.||.:|||||...|:|:|:|.:||:..|:.|. :.|:..:.|.
Yeast   387 LELKNHRNDTIMKYPLGPDNVIYRGSILRNTQNVVGMVIFSGEETKIRMNALKNPRTKAPKLQRK 451

  Fly   454 LNFIIIGIVLFLVSICALFA-IGCAIWEGLIGQHFQLYL----PWEHIIPKD--YIPTGATVIGL 511
            :|.||: .::|:|:..:||: :|..:       |.:.|:    .| ::...|  ..||
Yeast   452 INMIIV-FMVFVVATISLFSYLGHVL-------HKKKYIDQNKAW-YLFQADAGVAPT------- 500

  Fly   512 LVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMYYPTTNTYAKARTTTLNEELGQIQYI 576
              ..|:.|:.|||:|:||||::|:|:.|||.::.||.:||:..|||..::||.|:.|||||:.||
Yeast   501 --IMSFIIMYNTVIPLSLYVTMEIIKVVQSKMMEWDIDMYHAETNTPCESRTATILEELGQVSYI 563

  Fly   577 FSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNSNTNNRPSPLSGAIVAP 641
            |||||||||.|.|.|.|.|:.|.|:...:||...|        ..|:::..|      :.::..|
Yeast   564 FSDKTGTLTDNKMIFRKFSLCGSSWLHNVDLGNSE--------DNFEDNRDN------TNSLRLP 614

  Fly   642 PAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMDASEKRPGLKHVRYSAP 706
            |.|       |..      :|:.|.|.|:..|| ||    :..|..|::... ||.|.:.   ..
Yeast   615 PKA-------HNG------SSIDVVSIGDQNVL-DR----LGFSDAPIEKGH-RPSLDNF---PK 657

  Fly   707 SRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLSRTSSCDKVKILHDSQQ 771
            ||:.....|..|....|...:....|.:...:|            :|.|
Yeast   658 SRNSIEYKGNSSAIYTGRPSMRSLFGKDNSHLS------------KQAS---------------- 694

  Fly   772 TETHNMHHSSHNRKRLVKIRFKKAPSTATLGVVISQLEHHLDEEPQSTSTLSTTKHHHHRSKAFA 836
                                            |||.                        |:.|:
Yeast   695 --------------------------------VISP------------------------SETFS 703

  Fly   837 TNWSSSPHRVHALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVFFRLLALCHTVMAETV- 900
            .|..||...:..:|.               |...|.      .:.:..||..|||||:.:.:..
Yeast   704 ENIKSSFDLIQFIQR---------------YPTALF------SQKAKFFFLSLALCHSCLPKKTH 747

  Fly   901 -----DGKLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVM-------GQTEEYELLNILD 953
                 :..:|||:.||||.|||:|||:.|::...|....:||:..       .:.|.||:||.:|
Yeast   748 NESIGEDSIEYQSSSPDELALVTAARDLGYIVLNRNAQILTIKTFPDGFDGEAKLENYEILNYID 812

  Fly   954 FNNVRKRMSVILR---RGDSMVLYCKGADNVIYDRLHGGQ------------------------- 990
            ||:.||||||::|   :.:.::|.||||||||.:|||..:
Yeast   813 FNSQRKRMSVLVRMPNQPNQVLLICKGADNVIMERLHDRELAAKKMADICTSTKERKDAEAELVL 877

  Fly   991 -----------EDLKARTQ---------------------------------------------- 998
                       |:..|||.
Yeast   878 QQRKSLERMVDEEAMARTSLRNSLSSVPRASLSLQAVRKSLSMKNSRTRDPEKQIDSIDQFLETV 942

  Fly   999 ------------------------------------------------------------DH--- 1000
                                                                        :|
Yeast   943 KKSDQEIGSVVNKSRKSLHKQQIEKYGPRISIDGTHFPNNNVPIDTRKEGLQHDYDTEILEHIGS 1007

  Fly  1001 -----------------LNKFAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAI 1048
                             :::|:.||||||..|.:.:....|.:|..|..:|..|:..|:.|::..
Yeast  1008 DELILNEEYVIERTLQAIDEFSTEGLRTLVYAYKWIDIGQYENWNKRYHQAKTSLTDRKIKVDEA 1072

  Fly  1049 YEEIESEMQLVGVTAIEDKLQDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELAD 1113
            ..|||..:.|:|||||||||||||.::|..::.||||:|:|||||:||||||||||.|:.|....
Yeast  1073 GAEIEDGLNLLGVTAIEDKLQDGVSEAIEKIRRAGIKMWMLTGDKRETAINIGYSCMLIKDYSTV 1137

  Fly  1114 VFIVDGNSVEEVEKQLRQFKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNL 1178
            |.:.                                    .:|.|:      :::.:|..||.
Yeast  1138 VILT------------------------------------TTDENI------ISKMNAVSQEV-- 1158

  Fly  1179 PPTPPPPPAISVVTFRWDEKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNG 1243
                                                       :.|..|..||        |::|
Yeast  1159 -------------------------------------------DSGNIAHCVV--------VIDG 1172

  Fly  1244 HSLVHCL-SPELENKFLDIASQCKAVICCRVTPLQKALVVELIKRA-KNAVTLAIGDGANDVSMI 1306
            .::.... :|...:.|:::.::..:|||||.:|.||||:|..|:.. .|.|||||||||||::||
Yeast  1173 ATMAMFEGNPTYMSVFVELCTKTDSVICCRASPSQKALMVSNIRNTDPNLVTLAIGDGANDIAMI 1237

  Fly  1307 KAAHIGVGISGQEGLQAVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHC 1371
            ::|.|||||:|:|||||...|||||.|||:|.:||.||||::|.|..||:...|||...|.....
Yeast  1238 QSADIGVGIAGKEGLQASRVSDYSIGQFRFLLKLLFVHGRYNYIRTSKFMLCTFYKEITFYFTQL 1302

  Fly  1372 WYSLFCGFSAQTVFDPMFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIR 1436
            .|..:..||..::::|..:|::|..:||||||.:|:||:|:.....|..|.||:.|..|:.||..
Yeast  1303 IYQRYTMFSGSSLYEPWSLSMFNTLFTSLPVLCIGMFEKDLKPMTLLTVPELYSYGRLSQGFNWL 1367

  Fly  1437 EFIYSVLHGAFTSLVLFLIPYGVYKDGVSANGFVVSDH--MTLGAV--VATILIVDNTAQ-ISLY 1496
            .|:..|:.....||::..:  .|...|:|:    :||:  ..||.:  .|.:.:::..:| :.::
Yeast  1368 IFMEWVILATTNSLIITFL--NVVMWGMSS----LSDNTMYPLGLINFTAIVALINVKSQFVEMH 1426

  Fly  1497 TSYWTVVNHVTI--WGSLVW-------------YFVLDYFYNYVIGGPYVGSLTQAMKDLTFWVT 1546
            ...|.....|.:  .|.|||             |.|...|||: .|           ||:|||.|
Yeast  1427 NRNWLAFTSVVLSCGGWLVWCCALPILNNTDQIYDVAYGFYNH-FG-----------KDITFWCT 1479

  Fly  1547 MLITVMALVAPVLAYKFYLLDVHPSLSD---KIRQKS--LKKIHSRASSDVR------------- 1593
            .|:..:..:...:.||.:.:.:.||.||   ::.|||  .||:...|.|::|
Yeast  1480 SLVLALLPITLDIVYKTFKVMIWPSDSDIFAELEQKSDIRKKLELGAYSEMRQGWTWDKDPSTFT 1544

  Fly  1594 ----------RT---ASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMHKLPQDFAFPLGLGTKKT 1645
                      ||   ||::....|:.|             :::|.:.|...::|   .|..:||:
Yeast  1545 RYTDKVLSRPRTNSRASAKTHNSSIYS-------------MSNGNVDHSSKKNF---FGNSSKKS 1593

  Fly  1646 ----QVLHN-------NLNSADGPNSKGNNVT 1666
                :||.:       :|.:....:|.|.|:|
Yeast  1594 SERYEVLPSGKLIKRPSLKTQSSKDSIGGNIT 1625

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ATP8B	NP_731669.2	PhoLip_ATPase_N	195..260	CDD:292826	25/64 (39%)
		Cation_ATPase	891..985	CDD:289987	44/109 (40%)
		HAD_like	<1261..1324	CDD:304363	37/63 (59%)
		PhoLip_ATPase_C	1324..1571	CDD:292829	81/266 (30%)
DNF3	NP_013885.1	P-type_ATPase_APLT_Dnf-like	140..1386	CDD:319770	414/1499 (28%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0206
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S2398
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000076
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.760