Protein Alignment ATP8B and DNF2

DIOPT Version :9

• Sequence 1: NP_731669.2
• Gene: ATP8B / 41469
• FlyBaseID: FBgn0037989
• Length: 1726
• Species: Drosophila melanogaster
• Sequence 2: NP_010378.1
• Gene: DNF2 / 851667
• SGDID: S000002500
• Length: 1612
• Species: Saccharomyces cerevisiae
• Alignment Length: 1915
• Identity: 508/1915 - (26%)
• Similarity: 775/1915 - (40%)
• Gaps: 631/1915 - (32%)

  Fly    50 VDEPGIEAPEAS--CSAPSKRTRWFQFARSS----------------KNRRKRLHCNEDEDVKQP 96
            ||:...|:..||  .|:.|.....|||.:.|                |.:.|.:     :|:..|
Yeast    12 VDDIEHESGSASNGLSSMSPFDDSFQFEKPSSAHGNIEVAKTGGSVLKRQSKPM-----KDISTP 71

  Fly    97 ERRNSDLEG----SPRACLAQPEGMNIGSSTQTIATPLMIGDSFYSLAPGGGNPPPNKSDDQELY 157
            :......:|    |....:...:.:| |..|:.......:.|..:|..   ..|.||....:::.
Yeast    72 DLSKVTFDGIDDYSNDNDINDDDELN-GKKTEIHEHENEVDDDLHSFQ---ATPMPNTGGFEDVE 132

  Fly   158 KQRDPSVTSESKSVPSLRRLS-QIRRRRSSYYFSENERRIRANDKEF-NA--QFK---------- 208
            ...:....::|::...|:|:. ..||.:|........:.::...|.| ||  :|.
Yeast   133 LDNNEGSNNDSQADHKLKRVRFGTRRNKSGRIDINRSKTLKWAKKNFHNAIDEFSTKEDSLENSA 197

  Fly   209 -----------YHN--------------------NYIKTSKYSLFTFLPFNLLEQFQRLANFYFL 242
                       |:|                    |.|:|:||:..||.|.|:|.||...||.|||
Yeast   198 LQNRSDELRTVYYNLPLPEDMLDEDGLPLAVYPRNKIRTTKYTPLTFFPKNILFQFHNFANIYFL 262

  Fly   243 CLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTL--------- 298
            .||:|..........|...::|||.::.:||:||..:|.:|.:.|.:|||.::..|
Yeast   263 ILLILGAFQIFGVTNPGFASVPLIVIVIITAIKDGIEDSRRTVLDLEVNNTRTHILSGVKNENVA 327

  Fly   299 ------------------------------------------------RNGK------------- 302
                                                            ||.:
Yeast   328 VDNVSLWRRFKKANTRALIKIFEYFSENLTAAGREKKLQKKREELRRKRNSRSFGPRGSLDSIGS 392

  Fly   303 --------------------------------LVE-----------AK--WSEVQVGDVIRLDNN 322
                                            ||:           ||  |..|:|||::|:.||
Yeast   393 YRMSADFGRPSLDYENLNQTMSQANRYNDGENLVDRTLQPNPECRFAKDYWKNVKVGDIVRVHNN 457

  Fly   323 QFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHD----SLWNFNGEIICE 383
            ..:.||.:|||||:.:|.|::||..||||||||.:|.|..:..:....|    ..|     :..|
Yeast   458 DEIPADMILLSTSDVDGACYVETKNLDGETNLKVRQSLKCSKIIKSSRDITRTKFW-----VESE 517

  Fly   384 RPNNLLNKFDGTLIWRG-QRFALDNE-----KILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGK 442
            .|:..|..:.|...|:. |...:.||     .:|||||.||||:|..|:|:|.|.|||:|.|:|.
Yeast   518 GPHANLYSYQGNFKWQDTQNGNIRNEPVNINNLLLRGCTLRNTKWAMGMVIFTGDDTKIMINAGV 582

  Fly   443 TQFKSTGVDRLLNFIII--GIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTG 505
            |..|.:.:.|.|||.:|  .::||::...|          |::...:....|..    :||...|
Yeast   583 TPTKKSRISRELNFSVILNFVLLFILCFTA----------GIVNGVYYKQKPRS----RDYFEFG 633

  Fly   506 -----ATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMY-----YPTTNTYAK 560
                 |:..|.:.|:...|:..::||||||:|||:|:..|:..|..|..:|     ||.|
Yeast   634 TIGGSASTNGFVSFWVAVILYQSLVPISLYISVEIIKTAQAIFIYTDVLLYNAKLDYPCT----- 693

  Fly   561 ARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNS 625
            .::..::::||||:|||||||||||||:|.|.||:|||.|||...   |..|..:.::|.:
Yeast   694 PKSWNISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAY---TEALAGLRKRQGV---- 751

  Fly   626 NTNNRPSPLSGAIVAPPAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMD 690
                                                                   |:|
Yeast   752 -------------------------------------------------------DVE------- 754

  Fly   691 ASEKRPGLKHVRYSAPSRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLS 755
             ||.|                                     .|:..|:              ..
Yeast   755 -SEGR-------------------------------------REKEEIA--------------KD 767

  Fly   756 RTSSCDKVKILHDSQQTETHNMHHSSHNRKRLVKIRFKKAPSTATLGVVISQLEHHLDEEPQSTS 820
            |.:..|:::.:.|:.|.                      .|...|.      :...:.|:.:.:|
Yeast   768 RETMIDELRSMSDNTQF----------------------CPEDLTF------VSKEIVEDLKGSS 804

  Fly   821 TLSTTKHHHHRSKAFATNWSSSPHRVHALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVF 885
                   ..|:.|..                                            ||   |
Yeast   805 -------GDHQQKCC--------------------------------------------EH---F 815

  Fly   886 FRLLALCHTVMAETVDG---KLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYE 947
            ...|||||:|:.|....   ||:.:||||||:||||.||..|:.|...:.:.:.:|:.|..:|::
Yeast   816 LLALALCHSVLVEPNKDDPKKLDIKAQSPDESALVSTARQLGYSFVGSSKSGLIVEIQGVQKEFQ 880

  Fly   948 LLNILDFNNVRKRMSVILRRGDS-------MVLYCKGADNVIYDRLHGGQED--LKARTQDHLNK 1003
            :||:|:||:.|||||.|::...|       .:|.|||||:|||.||...|.|  |..:|..||.:
Yeast   881 VLNVLEFNSSRKRMSCIIKIPGSTPKDEPKALLICKGADSVIYSRLDRTQNDATLLEKTALHLEE 945

  Fly  1004 FAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKL 1068
            :|.||||||.||:|.||...|..|......||.|:.:||::|:.:.:.||.|:.|:|.|||||:|
Yeast   946 YATEGLRTLCLAQRELTWSEYERWVKTYDVAAASVTNREEELDKVTDVIERELILLGGTAIEDRL 1010

  Fly  1069 QDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIV--DGNSVEEVEKQLRQ 1131
            |||||.|||.|..||||:|||||||.|||||||:||.:|.::: ::.:|  .|..|||.
Yeast  1011 QDGVPDSIALLAEAGIKLWVLTGDKVETAINIGFSCNVLNNDM-ELLVVKASGEDVEEF------ 1068

  Fly  1132 FKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRW- 1195
                                      ..||:.|.....:.:::|                .|..
Yeast  1069 --------------------------GSDPIQVVNNLVTKYLRE----------------KFGMS 1091

  Fly  1196 --DEKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLS-PELENK 1257
              :|:.|:.|             .|.|...|           .||::::|.:|...|: .|:..|
Yeast  1092 GSEEELKEAK-------------REHGLPQG-----------NFAVIIDGDALKVALNGEEMRRK 1132

  Fly  1258 FLDIASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQ 1322
            ||.:...||||:||||:|.|||.||:|:|:..:.:|||||||:|||:||::|.:||||:|:||.|
Yeast  1133 FLLLCKNCKAVLCCRVSPAQKAAVVKLVKKTLDVMTLAIGDGSNDVAMIQSADVGVGIAGEEGRQ 1197

  Fly  1323 AVLSSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDP 1387
            ||:.|||:|.||||:.||:||||:|.|.|:.:.:..|||||..|||...||.::..|....:|:.
Yeast  1198 AVMCSDYAIGQFRYVTRLVLVHGKWCYKRLAEMIPQFFYKNVIFTLSLFWYGIYNNFDGSYLFEY 1262

  Fly  1388 MFISVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVL 1452
            .:::.|||.:||:||:.|.|.:|||||..|:..|:||..|:..:.:|..:|::.:|.|.:.|::.
Yeast  1263 TYLTFYNLAFTSVPVILLAVLDQDVSDTVSMLVPQLYRVGILRKEWNQTKFLWYMLDGVYQSVIC 1327

  Fly  1453 FLIPYGVYKDG--VSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWY 1515
            |..||..|...  |:.||..:.....:|..|..|.:......:.:....|.            |:
Yeast  1328 FFFPYLAYHKNMVVTENGLGLDHRYFVGVFVTAIAVTSCNFYVFMEQYRWD------------WF 1380

  Fly  1516 ---FV---LDYFYNYVIGGPYVGS---------LTQAMKDLTFWVTMLITVMALVAP-----VLA 1560
               |:   |..||.:.  |.:..|         ..:......:|..:.:.|:..:.|     .:.
Yeast  1381 CGLFICLSLAVFYGWT--GIWTSSSSSNEFYKGAARVFAQPAYWAVLFVGVLFCLLPRFTIDCIR 1443

  Fly  1561 YKFYLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGK 1625
            ..||..|:          :.::::..|...|:                    :.:|:.....|..
Yeast  1444 KIFYPKDI----------EIVREMWLRGDFDL--------------------YPQGYDPTDPSRP 1478

  Fly  1626 IMHKLP--QDFAFPLGLGTKKTQVLHNN------------LNSADGPNSKGNNVTGHHMVNNNTN 1676
            .::::.  .||..|:.|.|....|.|:.            ||...| :.||..|       :.|.
Yeast  1479 RINEIRPLTDFKEPISLDTHFDGVSHSQETIVTEEIPMSILNGEQG-SRKGYRV-------STTL 1535

  Fly  1677 MRHNQ--------NQNHSSMADITADGRGN 1698
            .|.:|        |....||    |..|||
Yeast  1536 ERRDQLSPVTTTNNLPRRSM----ASARGN 1561

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ATP8B	NP_731669.2	PhoLip_ATPase_N	195..260	CDD:292826	28/108 (26%)
		Cation_ATPase	891..985	CDD:289987	45/103 (44%)
		HAD_like	<1261..1324	CDD:304363	37/62 (60%)
		PhoLip_ATPase_C	1324..1571	CDD:292829	78/268 (29%)
DNF2	NP_010378.1	ATPase-Plipid	229..1456	CDD:273734	443/1550 (29%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	151	1.000	Domainoid score	I929
eggNOG	1	0.900	-	-		E2759_KOG0206
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000076
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100089
Panther	1	1.100	-	-	O	PTHR24092
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.810