Protein Alignment ATP8B and DNF2

DIOPT Version :10

• Sequence 1: NP_731669.2
• Gene: ATP8B / 41469
• FlyBaseID: FBgn0037989
• Length: 1726
• Species: Drosophila melanogaster
• Sequence 2: NP_010378.1
• Gene: DNF2 / 851667
• SGDID: S000002500
• Length: 1612
• Species: Saccharomyces cerevisiae
• Alignment Length: 1912
• Identity: 510/1912 - (26%)
• Similarity: 775/1912 - (40%)
• Gaps: 625/1912 - (32%)

  Fly    50 VDEPGIEAPEAS--CSAPSKRTRWFQFARSS----------------KNRRKRLHCNEDEDVKQP 96
            ||:...|:..||  .|:.|.....|||.:.|                |.:.|.:     :|:..|
Yeast    12 VDDIEHESGSASNGLSSMSPFDDSFQFEKPSSAHGNIEVAKTGGSVLKRQSKPM-----KDISTP 71

  Fly    97 ERRNSDLEG----SPRACLAQPEGMNIGSSTQTIATPLMIGDSFYSLAPGGGNPPPNKSDDQELY 157
            :......:|    |....:...:.:| |..|:.......:.|..:|..   ..|.||....:::.
Yeast    72 DLSKVTFDGIDDYSNDNDINDDDELN-GKKTEIHEHENEVDDDLHSFQ---ATPMPNTGGFEDVE 132

  Fly   158 KQRDPSVTSESKSVPSLRRLS-QIRRRRSSYYFSENERRIRANDKEF-NA--QFK---------- 208
            ...:....::|::...|:|:. ..||.:|........:.::...|.| ||  :|.
Yeast   133 LDNNEGSNNDSQADHKLKRVRFGTRRNKSGRIDINRSKTLKWAKKNFHNAIDEFSTKEDSLENSA 197

  Fly   209 -----------YHN--------------------NYIKTSKYSLFTFLPFNLLEQFQRLANFYFL 242
                       |:|                    |.|:|:||:..||.|.|:|.||...||.|||
Yeast   198 LQNRSDELRTVYYNLPLPEDMLDEDGLPLAVYPRNKIRTTKYTPLTFFPKNILFQFHNFANIYFL 262

  Fly   243 CLLVLQLIPAISSLTPVTTAIPLIGVLTLTAVKDAYDDIQRHISDSQVNNRKSKTL--------- 298
            .||:|..........|...::|||.::.:||:||..:|.:|.:.|.:|||.::..|
Yeast   263 ILLILGAFQIFGVTNPGFASVPLIVIVIITAIKDGIEDSRRTVLDLEVNNTRTHILSGVKNENVA 327

  Fly   299 ------------------------------------------------RNGK------------- 302
                                                            ||.:
Yeast   328 VDNVSLWRRFKKANTRALIKIFEYFSENLTAAGREKKLQKKREELRRKRNSRSFGPRGSLDSIGS 392

  Fly   303 --------------------------------LVE-----------AK--WSEVQVGDVIRLDNN 322
                                            ||:           ||  |..|:|||::|:.||
Yeast   393 YRMSADFGRPSLDYENLNQTMSQANRYNDGENLVDRTLQPNPECRFAKDYWKNVKVGDIVRVHNN 457

  Fly   323 QFVAADTLLLSTSEPNGLCFIETAELDGETNLKAKQCLTETIELGDRHD----SLWNFNGEIICE 383
            ..:.||.:|||||:.:|.|::||..||||||||.:|.|..:..:....|    ..|     :..|
Yeast   458 DEIPADMILLSTSDVDGACYVETKNLDGETNLKVRQSLKCSKIIKSSRDITRTKFW-----VESE 517

  Fly   384 RPNNLLNKFDGTLIWRG-QRFALDNE-----KILLRGCVLRNTQWCYGVVVFAGVDTKLMQNSGK 442
            .|:..|..:.|...|:. |...:.||     .:|||||.||||:|..|:|:|.|.|||:|.|:|.
Yeast   518 GPHANLYSYQGNFKWQDTQNGNIRNEPVNINNLLLRGCTLRNTKWAMGMVIFTGDDTKIMINAGV 582

  Fly   443 TQFKSTGVDRLLNFIII--GIVLFLVSICALFAIGCAIWEGLIGQHFQLYLPWEHIIPKDYIPTG 505
            |..|.:.:.|.|||.:|  .::||::...|          |::...:....|..    :||...|
Yeast   583 TPTKKSRISRELNFSVILNFVLLFILCFTA----------GIVNGVYYKQKPRS----RDYFEFG 633

  Fly   506 -----ATVIGLLVFFSYAIVLNTVVPISLYVSVEVIRFVQSFLINWDEEMY-----YPTTNTYAK 560
                 |:..|.:.|:...|:..::||||||:|||:|:..|:..|..|..:|     ||.|
Yeast   634 TIGGSASTNGFVSFWVAVILYQSLVPISLYISVEIIKTAQAIFIYTDVLLYNAKLDYPCT----- 693

  Fly   561 ARTTTLNEELGQIQYIFSDKTGTLTQNIMTFNKCSINGRSYGDVIDLRTGELVEITEQQTIFQNS 625
            .::..::::||||:|||||||||||||:|.|.||:|||.|||...   |..|..:.::|.:
Yeast   694 PKSWNISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAY---TEALAGLRKRQGV---- 751

  Fly   626 NTNNRPSPLSGAIVAPPAAPPPIILVHKAEVHAKKTSMVVTSSGEAQVLPDRPRSDIERSAPPMD 690
                                                                   |:|
Yeast   752 -------------------------------------------------------DVE------- 754

  Fly   691 ASEKRPGLKHVRYSAPSRSQDADAGRLSPRLDGSGGLSPPVGNEERRISGGFKRSGAGCMQRQLS 755
             ||.|                                     .|:..|:              ..
Yeast   755 -SEGR-------------------------------------REKEEIA--------------KD 767

  Fly   756 RTSSCDKVKILHDSQQTETHNMHHSSHNRKRLVKIRFKKAPSTATLGVVISQLEHHLDEEPQSTS 820
            |.:..|:::.:.|:.|.                      .|...|.      :...:.|:.:.:|
Yeast   768 RETMIDELRSMSDNTQF----------------------CPEDLTF------VSKEIVEDLKGSS 804

  Fly   821 TLSTTKHHHHRSKAFATNWSSSPHRVHALQSVDFSANPHHESDFRWYDRTLLDAVRSDEEHSHVF 885
                   ..|:.|..                                            ||   |
Yeast   805 -------GDHQQKCC--------------------------------------------EH---F 815

  Fly   886 FRLLALCHTVMAETVDG---KLEYQAQSPDEAALVSAARNFGFVFRTRTPNSITIEVMGQTEEYE 947
            ...|||||:|:.|....   ||:.:||||||:||||.||..|:.|...:.:.:.:|:.|..:|::
Yeast   816 LLALALCHSVLVEPNKDDPKKLDIKAQSPDESALVSTARQLGYSFVGSSKSGLIVEIQGVQKEFQ 880

  Fly   948 LLNILDFNNVRKRMSVILRRGDS-------MVLYCKGADNVIYDRLHGGQED--LKARTQDHLNK 1003
            :||:|:||:.|||||.|::...|       .:|.|||||:|||.||...|.|  |..:|..||.:
Yeast   881 VLNVLEFNSSRKRMSCIIKIPGSTPKDEPKALLICKGADSVIYSRLDRTQNDATLLEKTALHLEE 945

  Fly  1004 FAGEGLRTLALAERRLTEQYYNDWRSRQQEAALSMDSREQKLNAIYEEIESEMQLVGVTAIEDKL 1068
            :|.||||||.||:|.||...|..|......||.|:.:||::|:.:.:.||.|:.|:|.|||||:|
Yeast   946 YATEGLRTLCLAQRELTWSEYERWVKTYDVAAASVTNREEELDKVTDVIERELILLGGTAIEDRL 1010

  Fly  1069 QDGVPKSIANLQNAGIKIWVLTGDKQETAINIGYSCQLLTDELADVFIV--DGNSVEEVEKQLRQ 1131
            |||||.|||.|..||||:|||||||.|||||||:||.:|.::: ::.:|  .|..|||.
Yeast  1011 QDGVPDSIALLAEAGIKLWVLTGDKVETAINIGFSCNVLNNDM-ELLVVKASGEDVEEF------ 1068

  Fly  1132 FKESIKIYNRFRPGGFDPFDRLNSDSNMDPLSVTMTQTSAFMQETNLPPTPPPPPAISVVTFRWD 1196
                          |.||...:|:       .||......|....:                  :
Yeast  1069 --------------GSDPIQVVNN-------LVTKYLREKFGMSGS------------------E 1094

  Fly  1197 EKNKDNKGGPDSAECNNLFGDEKGSEDGGTASIVVDENTGFALVVNGHSLVHCLS-PELENKFLD 1260
            |:.|:.|             .|.|...|           .||::::|.:|...|: .|:..|||.
Yeast  1095 EELKEAK-------------REHGLPQG-----------NFAVIIDGDALKVALNGEEMRRKFLL 1135

  Fly  1261 IASQCKAVICCRVTPLQKALVVELIKRAKNAVTLAIGDGANDVSMIKAAHIGVGISGQEGLQAVL 1325
            :...||||:||||:|.|||.||:|:|:..:.:|||||||:|||:||::|.:||||:|:||.|||:
Yeast  1136 LCKNCKAVLCCRVSPAQKAAVVKLVKKTLDVMTLAIGDGSNDVAMIQSADVGVGIAGEEGRQAVM 1200

  Fly  1326 SSDYSIAQFRYLERLLLVHGRWSYYRMCKFLRYFFYKNFAFTLCHCWYSLFCGFSAQTVFDPMFI 1390
            .|||:|.||||:.||:||||:|.|.|:.:.:..|||||..|||...||.::..|....:|:..::
Yeast  1201 CSDYAIGQFRYVTRLVLVHGKWCYKRLAEMIPQFFYKNVIFTLSLFWYGIYNNFDGSYLFEYTYL 1265

  Fly  1391 SVYNLFYTSLPVLALGVFEQDVSDKNSLEFPRLYTPGLKSELFNIREFIYSVLHGAFTSLVLFLI 1455
            :.|||.:||:||:.|.|.:|||||..|:..|:||..|:..:.:|..:|::.:|.|.:.|::.|..
Yeast  1266 TFYNLAFTSVPVILLAVLDQDVSDTVSMLVPQLYRVGILRKEWNQTKFLWYMLDGVYQSVICFFF 1330

  Fly  1456 PYGVYKDG--VSANGFVVSDHMTLGAVVATILIVDNTAQISLYTSYWTVVNHVTIWGSLVWY--- 1515
            ||..|...  |:.||..:.....:|..|..|.:......:.:....|.            |:
Yeast  1331 PYLAYHKNMVVTENGLGLDHRYFVGVFVTAIAVTSCNFYVFMEQYRWD------------WFCGL 1383

  Fly  1516 FV---LDYFYNYVIGGPYVGS---------LTQAMKDLTFWVTMLITVMALVAP-----VLAYKF 1563
            |:   |..||.:.  |.:..|         ..:......:|..:.:.|:..:.|     .:...|
Yeast  1384 FICLSLAVFYGWT--GIWTSSSSSNEFYKGAARVFAQPAYWAVLFVGVLFCLLPRFTIDCIRKIF 1446

  Fly  1564 YLLDVHPSLSDKIRQKSLKKIHSRASSDVRRTASSRRGRRSVRSGYAFAHQEGFGRLITSGKIMH 1628
            |..|:          :.::::..|...|:                    :.:|:.....|...::
Yeast  1447 YPKDI----------EIVREMWLRGDFDL--------------------YPQGYDPTDPSRPRIN 1481

  Fly  1629 KLP--QDFAFPLGLGTKKTQVLHNN------------LNSADGPNSKGNNVTGHHMVNNNTNMRH 1679
            ::.  .||..|:.|.|....|.|:.            ||...| :.||..|       :.|..|.
Yeast  1482 EIRPLTDFKEPISLDTHFDGVSHSQETIVTEEIPMSILNGEQG-SRKGYRV-------STTLERR 1538

  Fly  1680 NQ--------NQNHSSMADITADGRGN 1698
            :|        |....||    |..|||
Yeast  1539 DQLSPVTTTNNLPRRSM----ASARGN 1561

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ATP8B	NP_731669.2	Haloacid Dehalogenase-like Hydrolases	211..>602	CDD:473868	162/547 (30%)
		Haloacid Dehalogenase-like Hydrolases	<885..1456	CDD:473868	237/585 (41%)
DNF2	NP_010378.1	ATPase-Plipid	229..1456	CDD:273734	445/1547 (29%)