DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment mfas and stab2

DIOPT Version :9

Sequence 1:	NP_788655.1	Gene:	mfas / 41455	FlyBaseID:	FBgn0260745	Length:	905	Species:	Drosophila melanogaster
Sequence 2:	XP_017210540.1	Gene:	stab2 / 565194	ZFINID:	ZDB-GENE-041210-336	Length:	2543	Species:	Danio rerio

Alignment Length:	994	Identity:	177/994 - (17%)
Similarity:	297/994 - (29%)	Gaps:	426/994 - (42%)

- Green bases have known domain annotations that are detailed below.

  Fly   227 GQERDGVTNVVRSPLFGQFQFSVNSCAEK-PNKHVCTKIVNQNGKKK---------------TLT 275
            |.:.|||.....:|.......:...||.. |||||||.....:|..:               |..
Zfish   220 GYQGDGVQCTSINPCLRSVCHANAVCAHTGPNKHVCTCTEGYSGDGRVCMPIDPCQTNLGNCTSG 284

  Fly   276 LTR-----------QC-----------------------CYGYGRPRNAD-FTTPCE-------- 297
            .||           :|                       |:.|.....|: .|..|.
Zfish   285 STRCVYDGPGKAHCECLEGFEKFVEGQGCSIIDLCKPDSCHKYATCATAEPGTVECNCREGFIGN 349

  Fly   298 -KIDIKDVEATASDMGAKQFLESARTAGELAD---MLGAGSG------KKVTLFVPNDAAFMEYR 352
             ||...|:....::|.:|   ...:..|:|:.   :....:|      ...|:|||.:.||   :
Zfish   350 GKICFGDIIQQLNEMNSK---PGGKWTGQLSSAITLFNQVTGWFLTARGPFTVFVPVNKAF---K 408

  Fly   353 GHHQQENNVEDAKAEASKPSIYKLHAVLGEVQLEDVPNEKLLQT----------ELPDQKIRINS 407
            |...:....::.||.    .:.|||.|.|||..|.:....|..|          :....|||::.
Zfish   409 GTSVKSLLADEMKAR----YLAKLHVVAGEVNSESLKKGILFHTLTGMGAESMIDAEQVKIRLHG 469

  Fly   408 YQLPAALGLEPYRFAANCVPIEKHDKLSEQALVHTLGGVLKPLTKNLMDIIRERADMSIMRTVLE 472
            .:...|| :|...||:|.: |...|||.: |:..|   |:....::|::|:.:....|..:::||
Zfish   470 SRKKGAL-VESDIFASNGM-IHLIDKLMD-AVPST---VISEKEESLLEILSKNGKFSQFKSLLE 528

  Fly   473 KTNLSAMLEDDKPVTIFVPTDAAFDKLEPHLRRALK--EGRGCASNILKNHLLDLTFCS---LAT 532
            ||||:.:||:|.|.|:|.||:.||..::|.....||  ||:.....:::||::..|..|   :.:
Zfish   529 KTNLATVLEEDGPYTLFAPTNVAFALMKPDYLAYLKSEEGKTKLLELMRNHIVSTTPLSAYVIVS 593

  Fly   533 VPGAKTTAYNLLGEPLLLNRTHRAANQTGPTPIYINNLAKIIDADIMGTNGVLHVIDTILPTESA 597
            .|.|.|.|..:|           ..|.|....|.:|. ..:|:.|:...||.||.::.:|...|.
Zfish   594 NPRAVTMAEQIL-----------TFNVTTAGQILVNG-ESVIELDVEAKNGRLHSLEGLLIPPSI 646

  Fly   598 LPMTSLMSQKNLTIFQRLLEASGYDDQFDDLDNVTIFAPTDKALQNTEWARMLKEQPELLNHNLD 662
            .|                                                        :|.|..|
Zfish   647 EP--------------------------------------------------------ILPHRCD 655

  Fly   663 LLEFLNYHVVKPMI-------KTCDLSEK-----------------SLPTVAGSSVRLNLYST-- 701
            :::.:.|  :.|.:       .||...|.                 |||::..|.:..:..:|
Zfish   656 IIKPIKY--MAPCVSCTLISKSTCPTGESLKAYRRGCVFNKKQLGLSLPSIGCSHICNDTKTTPQ 718

  Fly   702 ----------------------------------------------------------------- 701

Zfish   719 CCKGFFGPDCSPCPGGFTTPCSSHGTCSEGIDGNGTCQCEPKFKGSRCQYCADSNKYGPNCDKTC 783

  Fly   702 --------------------------------------------HALFSDVMNRATVNCARLVHF 722
                                                        ||..:.|.|:....|.....|
Zfish   784 WCIHGTCDNHPEASGKCKQGSCKDGYTGEYCELQTQPCGPNQPCHAHANCVSNKGAFTCVCKPGF 848

  Fly   723 ---------------------------------------------DDESCGSVLH---------- 732
                                                         |.:.|.::.:
Zfish   849 QGDGYMCMESDPCALPHRGGCSKNAICIKTGPGTHKCKCLSGWREDGDECQAINNCLDPSRGGCH 913

  Fly   733 -----------QVDRAL----------APPKN--------------------------------- 743
                       |:|.|.          ..|.|
Zfish   914 PNATCIYVGPGQIDCACKSGYHGNGRECEPVNQCVEQKGGCHFLATCQFLNPDGWHCVCEDGYAG 978

  Fly   744 -------NMLKLLEANPNYSKFLELVRKANLTQLLSNDSRSLTLLVPKNDIFEELNESGEG--SK 799
                   .:|:.:..||:...|.:.:.||.|:|||| :..:|||.||.....|::::..:.  ..
Zfish   979 DGKICYGTLLQEVSTNPDLLGFNQWISKAELSQLLS-ERENLTLFVPSAQAIEKMSKEDKDIWMT 1042

  Fly   800 PADPMALVKTHIVEDVVCCAGIIPTNWPFVRSIESISGQHLRITRDRRPKIENAG-VTKCDVVAT 863
            |::..:|||.||:......:.:..:..|..|.: |:..:.|.:.......|...| :|..|:.|.
Zfish  1043 PSNLPSLVKNHILSGSFTLSDLNTSPSPPPRLV-SLLKRTLPVYSTNDTTIVAGGKITFGDMAAK 1106

  Fly   864 NGILHEINDIIVPRPQRQQ 882
            ||::|.|:.:::|..|..:
Zfish  1107 NGVIHLIDTVLIPEQQMSE 1125

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mfas	NP_788655.1	FAS1	339..450	CDD:214719	35/120 (29%)
		Fasciclin	462..592	CDD:280607	41/134 (31%)
		Fasciclin	607..740	CDD:280607	24/343 (7%)
		Fasciclin	753..875	CDD:280607	34/124 (27%)
stab2	XP_017210540.1	EGF_3	239..268	CDD:289699	10/28 (36%)
		Fasciclin	374..495	CDD:280607	34/129 (26%)
		Fasciclin	518..643	CDD:280607	42/136 (31%)
		EGF_3	824..855	CDD:289699	6/30 (20%)
		EGF_3	910..941	CDD:289699	3/30 (10%)
		Fasciclin	1000..1119	CDD:280607	33/120 (28%)
		Fasciclin	1135..1252	CDD:280607
		EGF_3	1465..1501	CDD:289699
		EGF_3	1550..1586	CDD:289699
		Fasciclin	1616..1716	CDD:280607
		Fasciclin	1742..1873	CDD:280607
		EGF_3	2076..2111	CDD:289699
		EGF_3	2117..2154	CDD:289699
		Link_Domain	2188..2280	CDD:295393
		FAS1	2345..2438	CDD:214719
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	60	1.000	Domainoid score	I10602
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1830
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.940