Protein Alignment ClC-a and CLC-B

DIOPT Version :10

• Sequence 1: NP_788639.1
• Gene: ClC-a / 41428
• FlyBaseID: FBgn0051116
• Length: 1193
• Species: Drosophila melanogaster
• Sequence 2: NP_189353.1
• Gene: CLC-B / 822336
• AraportID: AT3G27170
• Length: 780
• Species: Arabidopsis thaliana
• Alignment Length: 759
• Identity: 188/759 - (24%)
• Similarity: 321/759 - (42%)
• Gaps: 160/759 - (21%)

  Fly   138 DKQRNKELLGKHSTRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALLSFIMDKGI-SICTNARI 201
            |.:.|:..|.||..| ||..:.:.::...:    |....|:|:...|::.:::..: :|.....:
plant    57 DYEINENDLFKHDWR-KRSKAQVLQYVFLK----WTLACLVGLFTGLIATLINLAVENIAGYKLL 116

  Fly   202 WLYRDLTSQPFVQ-YIAWVSLPVCLILFSAGFVHLIAPQSIGSGIPEMKTILRGVQLKEYLTFKT 265
            .:...||.:.:|. .:..|...:.|.|.::......||.:.|.||||:|..|.||.........|
plant   117 AVGHFLTQERYVTGLMVLVGANLGLTLVASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATT 181

  Fly   266 LVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLVTSFQGI-------YENESRNSEMLAA 323
            ::.|::|....:.:|:.||||||.|||.|.:|.||.:..|....|       :.|:....:::..
plant   182 MIVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGTDNHRIKWRWLRYFNNDRDRRDLITC 246

  Fly   324 ACAVGVGACFAAPVGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQNADTVRA--- 385
            ..|.||.|.|.:|||||||::|...|::.....||.||:.   |.|..:|..:.:..::.:.
plant   247 GSAAGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFST---AVVVVVLREFIEICNSGKCGLF 308

  Fly   386 ------LFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYVWVHRRYVLFMRSNKRMNKFLQKNR 444
                  :|..:..| :.:...::....|||::.|:.|:.|                 |..|.|..
plant   309 GKGGLIMFDVSHVT-YTYHVTDIIPVMLIGVIGGILGSLY-----------------NHLLHKVL 355

  Fly   445 FLY-----PGFLALLVSSISFPLGTGQFLAG---------------ELSTHEQVTQLFSNFTWSR 489
            .||     .|.:..::.|::..|.|...|.|               |:......:..|..|...:
plant   356 RLYNLINEKGKIHKVLLSLTVSLFTSVCLYGLPFLAKCKPCDPSIDEICPTNGRSGNFKQFHCPK 420

  Fly   490 ---DD-----LTVEQAAVVTHWMTSYTSVF--GNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKI 544
               :|     ||....||...:.::..:.|  |:|.|:.:...::.:....|..|||:|:|:..:
plant   421 GYYNDLATLLLTTNDDAVRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLFLPIILM 485

  Fly   545 GAGFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAA-FSGSVTHTVSVAVIIFEMTGQIT 608
            ||.:||::|..|           |..:.|..|.|||:|||| .:||:..|||:.||..|:|..:.
plant   486 GAAYGRMLGAAM-----------GSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNNLL 539

  Fly   609 HVVPVMIAVLVANAVAALLQPSIYDSIILIKKLPYL---PDLLPSSSGMYSIFVEDFMVRDVK-- 668
            .:...||.:|:|..|.....|||||.|:.:|.||:|   |:     ..|.::.|.:  :.|.|
plant   540 LLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLEANPE-----PWMRNLTVGE--LGDAKPP 597

  Fly   669 -YIWHGI-SYQKLKEVLKLNKTLRSLPLVDSPD---------NMILLGSVQRYELIKMIEKHIGR 722
             ....|: ....:.:||| |.|..:.|::|..:         ...|.|.:.|..|:|:::|
plant   598 VVTLQGVEKVSNIVDVLK-NTTHNAFPVLDEAEVPQVGLATGATELHGLILRAHLVKVLKK---- 657

  Fly   723 EKRMEVAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKKRAE 787
                    :|       .|.|:::.:|.|::.:.|.        |.|:.|:...|::.......|
plant   658 --------RW-------FLTEKRRTEEWEVREKFPW--------DELAEREDNFDDVAITSAEME 699

  Fly   788 TMHGSLAPRKSILKKTNSFNLKTYAQPMGHSPSITPYTTITGNS 831
             |:..|.|      .||:                ||||.:...|
plant   700 -MYVDLHP------LTNT----------------TPYTVMENMS 720

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ClC-a	NP_788639.1	ClC_1_like	171..646	CDD:239655	140/526 (27%)
		CBS_pair_SF	659..>718	CDD:449531	16/71 (23%)
		CBS repeat	664..715	CDD:341358	14/63 (22%)
		CBS_pair_SF	<1032..1096	CDD:449531
		CBS repeat	1053..1095	CDD:341358
CLC-B	NP_189353.1	ClC_6_like	54..575	CDD:239657	147/554 (27%)
		CBS_pair_voltage-gated_CLC_euk_bac	595..762	CDD:341367	36/177 (20%)
		CBS repeat	595..707	CDD:341367	29/146 (20%)
		CBS repeat	713..761	CDD:341367	3/8 (38%)