DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sbf and mtm-5

DIOPT Version :9

Sequence 1:	NP_477401.2	Gene:	Sbf / 41427	FlyBaseID:	FBgn0025802	Length:	1993	Species:	Drosophila melanogaster
Sequence 2:	NP_508888.2	Gene:	mtm-5 / 180792	WormBaseID:	WBGene00003477	Length:	1744	Species:	Caenorhabditis elegans

Alignment Length:	2095	Identity:	535/2095 - (25%)
Similarity:	854/2095 - (40%)	Gaps:	469/2095 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 DSDKEKTASNVGGQPTCGKIV-----QRFPEKDWPD------TPF------IEGIEWFCQPLGWS 61
            |.||.|:.      |.|..:.     :...|...||      :|.      ...|:.|.:|.||.
Worm     3 DPDKVKSG------PICDTVAVIVLEESDDENALPDVLHEVQSPHTSDNIPTSSIKKFARPRGWY 61

  Fly    62 LSYEKQEPKFFVSVLTDIDANKHYCACLSFHETVAITQTRSVDDEDETIGSSRLLGATPSSMDGI 126
            ........:||..:||.....:.....||..|            |||
Worm    62 NQSVSSPSEFFYQILTTERGTRRIAYVLSTWE------------EDE------------------ 96

  Fly   127 TTTSTPASITHHSVMYAPKCLVLISRLDCAETFKNCLGTI--------------------YTVYI 171
                       .::.:....:||||:....:.||..|..|                    |.:..
Worm    97 -----------KTLNFKAVSIVLISQNFHPKAFKEILLEISNDLRTPEFSSSSELIRFLTYELVE 150

  Fly   172 ENLAYGLETLIGNI-LGCIQVPPAGGPQVRFSIGAGDKQSLQPPQSSSLPTTGSGVHFLFKQLGI 235
            |.....:.|...:: ||...:|.:                         |.||..|..|||.||.
Worm   151 EGSTIEIRTKTLHVELGFELIPIS-------------------------PVTGKDVAMLFKMLGF 190

  Fly   236 KNVLILLCSVMTENKILFLSKCYWHLTDSCRALVALMYPFRYTHVYIPILPAPLTEVLSTPTPFI 300
            :||:.::.:::::.:|:..|.....|:....|:::|:|||.|.|..:.|||..|.|||.:||||:
Worm   191 QNVIKIIHALLSDCRIVLASSSLMRLSRCQNAILSLLYPFEYVHSCVTILPDSLAEVLESPTPFL 255

  Fly   301 MGIHSSLQTEITDLLDVIVVDLDGGLVTIPESLTPPVPILPSP--LWEQTQDLLSMILFPNLAQA 363
            :|:.|...|...|  :.|||.||.|.|.:|:.    ..|..|.  .:......|..::|...:|.
Worm   256 IGVLSEFVTSFGD--ENIVVYLDNGEVHVPDH----AEIYKSDDYYYNSLHQRLRDVMFTTTSQE 314

  Fly   364 DLAFPTLERPSAIAKTDAQIDKELRAIFMRLFAQLLQGYRSCLTIIRIHPK------PVITFHKA 422
            ||:.|..||   |...|..:||:|||.|:..||:||.||:..:...||...      ..:|||..
Worm   315 DLSIPNEER---IEVDDFILDKKLRACFIYYFAELLYGYQYYILYTRIKGNFEKKLTTSLTFHVG 376

  Fly   423 GFLGAR---DLIESEFLFRVLDSMFFTTFVNERGPPWRSSDAWDEL--YSSMNELLKSEAQNRNL 482
            .|.|.|   |::.|.    :|.|::|.||:..|..|.|..|.:||:  :..:::|:
Worm   377 AFRGFRKLTDMMSSS----LLKSVYFQTFILTRALPRRKHDLFDEISCFKELDQLI--------- 428

  Fly   483 VGRTQRFKGYFNFTFPSYFQILTHIQ-ELGRVLYENEGTLAHISYAQKVLRPPEGAFQRIHQPAF 546
                  ||.  |.|.....:|:.||. ||          :....|.:|.....:..|.:||..:.
Worm   429 ------FKQ--NSTSSESKKIIEHISCEL----------IQKERYMEKCSARKQEIFTKIHWISG 475

  Fly   547 PRISSEKVELI--IQEGIRKNGVPQRFHVTRNQHRIIPMGPRLPEALDVRPN-VQNSARRLEVLR 608
            ..::.....:|  ::..:|.|.:.|......|.|            .:...| .:..|.|:|.||
Worm   476 KELAQNNNSIIHTVKPKMRSNVILQAMLPVVNTH------------AEYHANQFEAYAHRIEALR 528

  Fly   609 ICVSYIFENRITDARKLLPAVMRTLMHRDARLILCREFFGYVHGNKAVLDHQQFELVVRFMNKAL 673
            .|::.|||.::..|.|.|.||..::.....|:.|||........:|  |..:|||.:...||.||
Worm   529 NCLAAIFEGKVAFASKSLDAVKSSMRFAPLRIELCRLLNQKCSHDK--LTDKQFEDIALLMNAAL 591

  Fly   674 QKSSGIDEYTVAAALLPMSTIFCRKLSTGVVQFAYTEIQDHAIWKNLQFWESTFFQDVQGQIKAL 738
            |.....|:..|..:|:.:|.::.||::.|:.|:.||.:|:|.:|||.:||.|.|:.:|...:.:.
Worm   592 QAECEEDKDGVVRSLMYLSNVYSRKVAQGMQQYMYTAVQEHKVWKNQRFWTSCFYYEVHEMLFSE 656

  Fly   739 YLLHRRQNEHQKEANCVLDEVPLEEPTALEITAEQLRKSPNIEEEKKAELAKSEESTLYSQAIHF 803
            .|...|:.......:.:       .|.|:|:.        |.::..:.||.|.|...:.:||.||
Worm   657 MLQKDRKITESLWCHTL-------RPCAMEMI--------NTDDTDQEELVKQENEMIQAQAKHF 706

  Fly   804 ANRMVSLLIPLDVNVDAASKPKPAFRLEENQSVSNSIMGSHSLSEHSDEGFEENNALEIGVTVG- 867
            ||.::||.|||                                   |:|.||..:|....:...
Worm   707 ANILISLQIPL-----------------------------------SEEFFEHEDAHRSVLNEKC 736

  Fly   868 KTISRFIDCVCTEGGVTSEHIRNLH-DMVPGVVHMHIESLEPVYLEAKRHPHVQKPKIQTPCLLP 931
            |.|...:|.:.   |||. .|..|. ..:...|..|:|||..||:|.....|::|.... |.|..
Worm   737 KWIVNTLDSIL---GVTG-RINGLSLSRIQTYVEAHVESLRDVYVEMSTGEHLKKGNFD-PVLAH 796

  Fly   932 GEDLVTDHLRCFLMPDGREDE-----TQCLIPAEGALFLTNYRVIFKGSPCDPLFCEQVIVRTFP 991
            ||.|::|.:.|:|:....|.|     .:.|:||:|:||||||||||||...|.......||:|.|
Worm   797 GEFLISDPIDCYLLTSIEESEMSLNRLENLLPADGSLFLTNYRVIFKGKSVDINATNGTIVQTIP 861

  Fly   992 IASL-----LKEKKISVLYLAHLDQTLTEGLQLRSSSFQLIKVAFD-PEVTPEQIESFRKILSKA 1050
            :.|:     |..||:....|......:...:.:|||....|.:||| .|:....||.|.:::...
Worm   862 LYSMESFKKLTNKKLIPTQLIEKGVKIEHIISIRSSCASSIIIAFDEDEINNMAIEKFLEVIETN 926

  Fly  1051 RHPFDEFEYFAFQSYGTMLQGVAPLKTKEKYSTLKGFAKKTLLRGAKKAGFKQKQQTKRKLVSDY 1115
            .|     ..|||  |.|............|:.||....:          ||.:|:...|::.|.
Worm   927 SH-----NSFAF--YNTRKDMKVVENGSHKFGTLNSAIR----------GFTKKKTDTRRIRSH- 973

  Fly  1116 DYGSADAQETQSIDDELEDGDEFETQNNAMPRLLTTKDVERMRERSYVQDWKRLGFDAESQRGFR 1180
               |:.....|...|::|:.|..  :.||             ..|..|.|:.|:|.:::..: .|
Worm   974 ---SSHRGSIQLSFDKMEELDYL--KKNA-------------HIRYAVIDYPRIGLNSKIVK-LR 1019

  Fly  1181 ISNANTSYATCRSYPAIIVAPVQCSDAAIMHLGRCFKGQRIPLPTWRHANGALLIRGGQPNS--- 1242
            :|::|..|..|.|||...:.|.:.:::.:..:.:.|...|:|:..|.:.|||||:|.....|
Worm  1020 MSHSNLDYTICPSYPGNFIVPSETNESELAKVAKGFVEHRLPVVVWMNENGALLVRASAFTSIDM 1084

  Fly  1243 ----KSVIGMLKNT---TGSTTNAHHDVTHYPEQDKYFLALINTMPKLTPLALNQYSGMNLSMSS 1300
                |.|:...:|.   |||.|.:        :|..:..|..|.......:|..:.....:.|:.
Worm  1085 VKKLKKVVNYRRNASKLTGSMTGS--------QQTLHSKASSNEESSSNIVAGAEIKSAEVQMNY 1141

  Fly  1301 LMGHSSSDDRQPLTPELSRKHKNNLDISDGNKSSQGGKGGTMKGNPKNSL-AHPFRKMRLYALGE 1364
            :...|:|..| .::..|..::        .:|.|....|.|:..|..|.. .....:..||.|.|
Worm  1142 IAKLSNSSQR-AVSYALPTQY--------ADKFSTFNDGCTLTQNNANGFPTTRIHRKALYVLLE 1197

  Fly  1365 KSQAKSNMNVDFCADFIPVDYPDIRQSRPAFKKLIRACMPSHNTNEADGQSFAKMVEQSDWLQQI 1429
            |... ..:.:|..|:.|.|......:.|.:.::..:.|  |......:..||.:....|:|.|.:
Worm  1198 KGHG-VKIPIDSNAEAIMVRSVKESELRRSLQRARQIC--SSEFQVENRTSFLESWNASNWPQCV 1259

  Fly  1430 SSLMQLSGAVVDLIDLQESSVMLSLEDGSDVTAQLSSIAQLCLDPYYRSLDGFRVLVEKEWLAFG 1494
            |.:::||.::|.|::|..|||.:.||.|..:|..|||::||..|||||:.|||:|||||||||||
Worm  1260 SRMIELSNSIVALMNLYNSSVAICLEAGRSITTILSSLSQLLSDPYYRTCDGFQVLVEKEWLAFG 1324

  Fly  1495 HRFAHRSNLKPSHANTNIAFAPTFLQFLDVVHQLQRQFPMAFEFNDFYLRFLAYHSVSCRFRTFL 1559
            |.|         |.:|..: :|:|:.|||.|:|:.:|:|.||||:.||:.||||||.:..||||:
Worm  1325 HYF---------HKDTETS-SPSFICFLDCVYQISQQYPTAFEFSYFYISFLAYHSTAGYFRTFI 1379

  Fly  1560 FDCELER--SDSGIAAMEDKRGSLNAKHMFGAGGMATNGSDDECSVYPLDIRSQRAPAPLNRIGH 1622
            .|||.:|  ||:....:.|...::|
Worm  1380 DDCEEKRLQSDANEFYLPDNLATIN---------------------------------------- 1404

  Fly  1623 SIFDYIERQHNKTPIFYNFLYS--GDKSVTLRPQNNVAALDLWCYYTNEELAQGAPYDLEVTTVD 1685
             ::::|:.::..:..|||.||.  ||..:   |.:::..:.:|.:.....|..|:|||:|..:.:
Worm  1405 -VWEFIKLRNRVSAAFYNELYEQIGDIVI---PSSSIPQIHMWPFLAETHLKYGSPYDIEPASHE 1465

  Fly  1686 DEIDLSETKGKRMVITAGYDNMEKCNPSAYVCLLSEVKQAETERGHLPQKWLQVWNSLEVPQLEP 1750
            .::           :...|:..|.         .|::...:.:..||.::       :..|:.:|
Worm  1466 QQL-----------VDPDYEEEED---------WSKLNNTDIDERHLNRR-------VRSPERDP 1503

  Fly  1751 -------VARNTSLGNIFVQTHQHKRSTLEIIMKGRLAGYQDKYFHPHRFEKHPYTT---PTNCN 1805
                   :.:.:.|..:| .....|.:|     .|...|.:..:      |..|:|.   |..|.
Worm  1504 ANMDMIRLLQKSYLTELF-DASDRKTTT-----NGESNGKETIH------ELTPFTVGARPVQCC 1556

  Fly  1806 HCTKLL--WGPVGYRCMDCGNSYHEKCTEHSM-----------KNCTKYKAIDGA--VGPPNVNM 1855
            :||.:|  |.. ...|..|....||.|...::           |.....|...||  :|.|....
Worm  1557 YCTNILTRWSK-AVHCKKCRIHVHEGCVNRNITIGNITHTWDAKPFEDIKMPSGAIQIGTPQAEK 1620

  Fly  1856 ---SQGDTASIASSAATTARTSSHHFYNQFSSNVAENRTHEGHLYKRGALLKGWKQRWFVLDSIK 1917
               |..:|.:..|.:..||.|            :....|  |:|.||||.||.|..|:|||....
Worm  1621 MLHSPNNTLTRESMSPPTANT------------IPPLCT--GYLSKRGAKLKLWVPRFFVLYPDS 1671

  Fly  1918 HQLRYYDTSED--TA--PKGIIELAEVQSVTAAQPAQIGAKGVDEKGFFDLKTSKRIYNFYAINA 1978
            .::.||:..|:  ||  |.|.|:|.:.:|....|..:        :|..:|....:.:...:.|.
Worm  1672 PKVYYYEDFENWKTAEKPSGCIDLVDFKSFNLEQTGR--------RGLIELHMKNKTHRLLSENI 1728

  Fly  1979 NLAQEWIEKLQACLQ 1993
            |.|..|.|.::..::
Worm  1729 NEAIRWKECIEQVIR 1743

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sbf	NP_477401.2	uDENN	1..91	CDD:214824	20/93 (22%)
		DENN	142..325	CDD:280329	55/203 (27%)
		dDENN	384..452	CDD:129037	27/76 (36%)
		SBF2	585..807	CDD:289132	65/222 (29%)
		PH-GRAM_MTMR5_MTMR13	933..1047	CDD:275396	44/124 (35%)
		Myotub-related	1161..1565	CDD:284109	132/414 (32%)
		C1	1791..1838	CDD:237996	15/62 (24%)
		PH_Sbf1_hMTMR5	1888..1993	CDD:269941	32/108 (30%)
		PH	1891..1992	CDD:278594	32/104 (31%)
mtm-5	NP_508888.2	uDENN	9..92	CDD:214824	17/88 (19%)
		DENN	105..278	CDD:214823	55/199 (28%)
		dDENN	332..405	CDD:129037	27/76 (36%)
		SBF2	504..708	CDD:289132	65/232 (28%)
		PH-GRAM_MTMR5_MTMR13	798..923	CDD:275396	44/124 (35%)
		PTPc	1005..1383	CDD:304379	129/408 (32%)
		C1	1546..1585	CDD:294036	12/39 (31%)
		PH	1648..1743	CDD:278594	31/102 (30%)
		PH-like	1648..1742	CDD:302622	31/101 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160166695
Domainoid	1	1.000	187	1.000	Domainoid score	I1985
eggNOG	1	0.900	-	-		E1_KOG1090
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H41810
Inparanoid	1	1.050	491	1.000	Inparanoid score	I776
Isobase	1	0.950	-	0	Normalized mean entropy	S4814
OMA	1	1.010	-	-		QHG45747
OrthoDB	1	1.010	-	-		D11535at33208
OrthoFinder	1	1.000	-	-		FOG0003022
OrthoInspector	1	1.000	-	-		oto18112
orthoMCL	1	0.900	-	-		OOG6_104786
Panther	1	1.100	-	-	LDO	PTHR10807
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R8681
SonicParanoid	1	1.000	-	-		X2012
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	17	16.790