DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mrp4 and NFT1

DIOPT Version :9

Sequence 1:	NP_650086.2	Gene:	Mrp4 / 41387	FlyBaseID:	FBgn0263316	Length:	1316	Species:	Drosophila melanogaster
Sequence 2:	NP_013029.3	Gene:	NFT1 / 853978	SGDID:	S000001811	Length:	1218	Species:	Saccharomyces cerevisiae

Alignment Length:	1067	Identity:	269/1067 - (25%)
Similarity:	480/1067 - (44%)	Gaps:	184/1067 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPRERSSPLSS----LMFCFALPVLFKGRKKTLEQKDLYRALKEHKSDSLGDRLCAAWDEQV 69
            |.||..::..|..:    |.|.....:::|..|..|...|::.....:||.::           :
Yeast   234 LVENENKKLPPAPTVFGLLTFSRIDRLIWKAYKHCLGNADIWDLDINNKSIAI-----------L 287

  Fly    70 AKNE-TPRLGRALTKVFGFH--LFITGVFL-LAQEFLTKVTQPICLIGVMAYFAGNDPDRSKAQL 130
            |..| :.:.||.|..:..:.  :||:.:|| ....||..|.. :.:..:::|.  |||   |::.
Yeast   288 ANFEMSSKKGRLLPNIICYFKAVFISQLFLAFVSSFLNFVPS-LLMPRILSYV--NDP---KSKS 346

  Fly   131 W-------AAGLIAGSVFSVCIGHPYMLGLLHLG-MKMRIALSSLIYRKALRLSRTALGDTTV-- 185
            |       ::.|::..:.:.|.|....||  ..| |::|..|.|.||.|.||  ||.|.|:|.
Yeast   347 WNLVSLYVSSMLVSKIIATTCRGQGLFLG--EKGTMQLRTVLISNIYSKTLR--RTILKDSTTSL 407

  Fly   186 ---------------------------GQVVNLLSNDVGRFDLVLINVHYLWIAPLELIAVTYLM 223
                                       ..|.|::|.|..:....: |..|| ......:.||.||
Yeast   408 QKNASTSFEENPDSSEAEPRKKSSRKDNSVNNVMSIDAFKVSEAM-NTFYL-ACEAVFMTVTALM 470

  Fly   224 --YLEIGISSMFGVAIMLLFLPFQSYLGKRTSVLRLRTALRTDERVRMMNEIISGIQVIKMYAWE 286
              |..:|.|:..|...:|..:|...:........:....:.||:|...::|.::.|:|||:.|||
Yeast   471 ILYSLLGWSAFAGTFALLAMIPLNFWCATFYGNYQADQLILTDKRTSGISEALNSIRVIKLLAWE 535

  Fly   287 KPFGKVVEMTRFNEMLCIKQ---VNYIRGILISFAMFLSRIFTSSSLIAFVLLGNILNAEKAFFV 348
            ..|.:.:...|..|:..:|:   :.::..::..|...|....|.|..|.|  ....|....||..
Yeast   536 NLFYQKIINVRDGEIRLLKKKATIFFLNHLIWFFGPTLVSAITFSVFIKF--QNQTLTPTIAFTA 598

  Fly   349 TAYYNILRRSVTMFFPQGISEFAELLVSVRRLEAFMHRPETKVRDKSKVKNANQKAESPNGDSPK 413
            .:.:.|||..:.. ....:|...:..:|:.|::.:::..||  |....::.:|.|
Yeast   599 LSLFAILRTPMDQ-IASTVSLLIQSFISLERIQDYLNESET--RKYEILEQSNTK---------- 650

  Fly   414 GNGIPENLIEFSQFQARWESHSLEPTLEDINLQLGRRKLVAVIGPVGAGKSSLIQAILGELPGES 478
                    ..|......||:......|::|::......|.|:|||.|:|||||:..:||||...|
Yeast   651 --------FGFEDASMEWEAAETSFKLKNISIDFKLNSLNAIIGPTGSGKSSLLLGLLGELNLLS 707

  Fly   479 GTLRI----------------NGSYSYAAQEPWLFTGTVRQNILFGLDWDKHRYRTVVKKCALER 527
            |.:.:                ..|.:|.:|.|||.:||::.|::||..::|.::..|:|.|.|::
Yeast   708 GKIYVPTVESRDDLEIGKDGMTNSMAYCSQTPWLISGTIKDNVVFGEIFNKQKFDDVMKSCCLDK 772

  Fly   528 DFELLPFGDKTIVGERGASLSGGQKARISLARAVYRRADIYLLDDPLSAVDTHVGRHLFDQCMRG 592
            |.:.:..|.:|.||:.|.||||||:.||:||||:|..:...:|||.|||||.....:::::|:.|
Yeast   773 DIKAMTAGIRTDVGDGGFSLSGGQQQRIALARAIYSSSRYLILDDCLSAVDPETALYIYEECLCG 837

  Fly   593 -YLRSELVILVTHQLQFL-EQADLIVIMDKGRISAMGTYSSMKRSGLDFAQLLTAPNKDAEDLDE 655
             .::....|:.:|.:..: ::||.:||:|:|.:.:.|..|.:.:|. :|  |..:.|.|:::...
Yeast   838 PMMKGRTCIITSHNISLVTKRADWLVILDRGEVKSQGKPSDLIKSN-EF--LRESINNDSKNTTH 899

  Fly   656 IDGAGGDGLDLLNVPSLSRRGSKNSKPSTRNNSFTSLSSMAESMAQEASLQMQETRVEGKIGLGL 720
                  :.:||..  |.:.:.:||..|...|:.        :.:..|.. ..:||::||.:....
Yeast   900 ------NQIDLKR--STTSKKTKNGDPEGGNSQ--------DEVCAEVE-NFEETKMEGSVKFSA 947

  Fly   721 YKEYLTSGSSWFMIFF----MVFLCLATQILCS----AADYFLSYWVD------------KNVDG 765
            ||        |...:|    :||:..::.||..    :..::|.||:|            :.|:|
Yeast   948 YK--------WLADYFGGLGVVFVFTSSSILIHGITLSQGFWLRYWLDTGSSGSKSTWLYRIVEG 1004

  Fly   766 QTDINTDPQDMYYFAALNVAVVVFTIVRTMLFYKMAMRSSTQLHNAMYQGITRAAMY----FFNT 826
            .::|        ||....:.:.:.:...|.....:|:.|.|.:...::..:..:.:|    |.|.
Yeast  1005 HSNI--------YFLLTYIIIGLVSSFLTSGKVWIAIISGTNVTKKIFAKLLSSILYAKLRFHNV 1061

  Fly   827 NPSGRILNRFSKDLGQLDEVLPSVMLDVVQLFLTLLGIIVVICITNPYYLILTLALAIIFYYIRE 891
            .|:|||:||||||:..:|:.|......:....:..|.||::|....|.:|:..:.|..::|.:..
Yeast  1062 TPTGRIMNRFSKDMDIIDQQLIPNFEGLSYSVVVCLWIILLIGYVTPQFLLFAIPLCALYYTVCT 1126

  Fly   892 FYLKTSRDVKRLEAVARSPIYSHLSATITGLPTIRALGAQKELIAEFDNLQDLHSSG---YYTFL 953
            .||:.||::||::.:..|||:...:..|.|:.|||||..::..|.:  :|..:..|.   :|..:
Yeast  1127 LYLRASRELKRIDNINISPIHQLFAEAIKGVTTIRALADERRFITQ--SLVAIDRSNAPFFYLNM 1189

  Fly   954 ATNRAFGYYLDCFCTLYI----VIIIL 976
            || ....|.:|...||.:    |:||:
Yeast  1190 AT-EWITYRVDIIGTLVLFSSSVMIIM 1215

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mrp4	NP_650086.2	CFTR_protein	12..1277	CDD:273530	267/1064 (25%)
		ABC_membrane	93..359	CDD:294371	75/308 (24%)
		ABCC_MRP_domain1	422..622	CDD:213217	74/217 (34%)
		ABC_membrane	735..978	CDD:294371	70/273 (26%)
		ABCC_MRP_domain2	1051..1272	CDD:213211
NFT1	NP_013029.3	MRP_assoc_pro	252..>1218	CDD:188098	265/1049 (25%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157344657
Domainoid	1	1.000	64	1.000	Domainoid score	I2425
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100026
Panther	1	1.100	-	-	O	PTHR24223
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.830