Protein Alignment Mrp4 and YBT1

DIOPT Version :9

• Sequence 1: NP_650086.2
• Gene: Mrp4 / 41387
• FlyBaseID: FBgn0263316
• Length: 1316
• Species: Drosophila melanogaster
• Sequence 2: NP_013052.1
• Gene: YBT1 / 850678
• SGDID: S000003971
• Length: 1661
• Species: Saccharomyces cerevisiae
• Alignment Length: 1448
• Identity: 447/1448 - (30%)
• Similarity: 730/1448 - (50%)
• Gaps: 218/1448 - (15%)

  Fly    19 PLSSL--MFCFAL--PVLFKGRKKTLEQKDLYRALKEHKSDSLGDRLCAAWDEQVAKNETPRLGR 79
            |::|:  ..|:|.  ..::|..|.:::.||::..:.:..|..:..:.....|.:|.:.
Yeast   282 PVTSIAGFICWAWLDSFVWKAHKVSIKVKDIWGLMMQDYSFFVVKKFRYFVDHKVKRK------- 339

  Fly    80 ALTKVFGFHL-FITGVFLLAQ---EFLTKVTQ---PICLIGVMAYFAGNDPDRSKAQ-----LWA 132
               ::|..:| |....:|:.|   .||..|..   .:.|..::.|.    .|:|.|.     .:.
Yeast   340 ---RIFSLNLFFFFSNYLVLQCFWAFLGSVLSFIPTVLLKRILEYV----EDQSSAPSNLAWFYV 397

  Fly   133 AGLIAGSVF-SVCIGHPYMLGLLHLGMKMRIALSSLIYRKALRL-------------------SR 177
            ..:..|.:. ::|.......| ..:.::|:..:.|.||.||||.                   .:
Yeast   398 TVMFVGRILVAICQAQALFFG-RRVCIRMKSIIISEIYTKALRRKISTNKTKPSNEDPQEINDQK 461

  Fly   178 TALGD------TTVGQVVNLLSNDVGRFDLVLINVHYLWIAPLELIAVTYLMYLEIGISSMFGVA 236
            :..||      ..:|.::||::.|..:...:...:|....|.:..:....|:|..:|.:::.||.
Yeast   462 SINGDEESTSSANLGAIINLMAIDAFKVSEICGYLHSFLEAFVMTVVALALLYRLLGFAAIVGVL 526

  Fly   237 IMLLFLPFQSYLGKRTSVLRLRTALRTDERVRMMNEIISGIQVIKMYAWEKPFGKVVEMTRFNEM 301
            |::..||....|.|....|:.:....||.|::.:||....|::||.::||:.|.|.:...|.||:
Yeast   527 IIVAMLPLNYKLAKYIGDLQKKNLAVTDNRIQKLNEAFQAIRIIKYFSWEENFEKDINTIRENEL 591

  Fly   302 LCIKQVNYIRGILISFAMFL----SRIFTSSSLIAFVLL-GNILNAEKAFFVTAYYNILRRSVTM 361
            ..:    .:|.|:.|.:.||    ..|.|::|...::.: |.:|....||...:.:.:||..:..
Yeast   592 SLL----LMRSIVWSISSFLWFVTPTIVTAASFAYYIYVQGEVLTTPVAFTALSLFTLLRDPLDR 652

  Fly   362 FFPQGISEFAELLVSVRRLEAFMHRPETKVRDKSKVKNANQKAESPNGDSPKGNGIPENLIEFSQ 426
            .... :|...:..||:.|::.|::..:||..|:..:        .|||          |...|..
Yeast   653 LSDM-LSFVVQSKVSLDRVQDFLNENDTKKYDQLTI--------DPNG----------NRFAFEN 698

  Fly   427 FQARWESHSLEPTLEDINLQLGRRKLVAVIGPVGAGKSSLIQAILGEL-------------PGES 478
            ....|:..:.:..|:|:|::....||..||||.|:||:||:.|:|||:             |.:.
Yeast   699 STISWDKDNQDFKLKDLNIEFKTGKLNVVIGPTGSGKTSLLMALLGEMYLLNGKVVVPALEPRQE 763

  Fly   479 GTLRING---SYSYAAQEPWLFTGTVRQNILFGLDWDKHRYRTVVKKCALERDFELLPFGDKTIV 540
            ..:..||   |.:|.:|..||...||:.||||...:::.||:.||:.|.|:||||:|..||.|.:
Yeast   764 LIVDANGTTNSIAYCSQAAWLLNDTVKNNILFNSPFNEARYKAVVEACGLKRDFEILKAGDLTEI 828

  Fly   541 GERGASLSGGQKARISLARAVYRRADIYLLDDPLSAVDTHVGRHLFDQCMRGYLRSE-LVILVTH 604
            ||:|.:||||||.|:|||||:|..|...||||.|||||:|....::|.|:.|.|..: ..|||:|
Yeast   829 GEKGITLSGGQKQRVSLARALYSNARHVLLDDCLSAVDSHTASWIYDNCITGPLMEDRTCILVSH 893

  Fly   605 QLQF-LEQADLIVIMDKGRISAMGTYSSMKRSGLDFAQ-------LLTAPNKDAEDLDEIDGAGG 661
            .:.. |..|:|:|:::.||:...|....|.:.|| |.:       :|:..|..|      :.|..
Yeast   894 NIALTLRNAELVVLLEDGRVKDQGDPIDMLQKGL-FGEDELVKSSILSRANSSA------NLAAK 951

  Fly   662 DGLDLLNVPSLSRRGSKNSKPSTRNNSF--------TSLSSMAESMAQEASLQMQETRVEGKIGL 718
            ....|.|:|::     |..:.|..|||.        .||.:.|| ..::..|..:||:.||.:||
Yeast   952 SSTSLSNLPAV-----KEQQVSVNNNSSHFEAKKLQKSLRTEAE-RTEDGKLIKEETKEEGVVGL 1010

  Fly   719 GLYKEYLTSGSSWFMIFFMVFLCLATQILCSAADYFLSYWVDKNV-------------------- 763
            .:||.||.....|.::.|:..|.|..|:|.....:::..|...||
Yeast  1011 DVYKWYLKIFGGWKIVSFLASLFLIAQLLYIGQSWWVRAWASHNVIAKIIPRAQRAIAFISKKAS 1075

  Fly   764 -----DGQTDIN-----TDPQD----MYY---FAALNVAVVVFTIVRTMLFYKMAMRSSTQLHNA 811
                 .|.:.|:     ..|..    |||   :..:..|..:....:|:|.:...:.:|.::.|.
Yeast  1076 HLIDWRGSSQISMASAENQPSSGHSTMYYLVLYLIIGFAQALLGAGKTILNFVAGINASRKIFNM 1140

  Fly   812 MYQGITRAAMYFFNTNPSGRILNRFSKDLGQLDEVLPSVMLDVVQLFLTLLGIIVVICITNPYYL 876
            :...:..:.:.||:..|:|||:||||||:..:|:.|...:.......:..|..:::|....|.:|
Yeast  1141 ILNKVLHSKIRFFDATPTGRIMNRFSKDIEAIDQELTPYIQGAFYSLIECLSTVILITFITPQFL 1205

  Fly   877 ILTLALAIIFYYIREFYLKTSRDVKRLEAVARSPIYSHLSATITGLPTIRALGAQKELIAEFDNL 941
            .:.:.::|::|::..||:..||::||.|:::|||||.|.|.|:.|:.||||.|.:...:.|  ||
Yeast  1206 SVAIVVSILYYFVGYFYMAGSRELKRFESISRSPIYQHFSETLVGVTTIRAFGDEGRFMQE--NL 1268

  Fly   942 QDLHSSG---YYTFLATNRAFGYYLDCFCTLYI----VIIILNYFINPPQSPGEVGLAITQAMGM 999
            ..:..:.   :|.::| ||...:.:|...:|.|    :.|:.|  ||...| |..|:::|.|:..
Yeast  1269 HKIDENNKPFFYLWVA-NRWLAFRIDMIGSLVIFGAGLFILFN--INNLDS-GMAGISLTYAISF 1329

  Fly  1000 TGMVQWAMRQSAELENTMTAVERVVEYDEIEPEGEFDSREGKKPSPSWPEKGEIIAEDLCLRYFP 1064
            |....|.:|..:|:|..|.:||||.||.|||.|...:.:|  .|.|.||:.|:|...||.|||.|
Yeast  1330 TEGALWLVRLYSEVEMNMNSVERVKEYMEIEQEPYNEHKE--IPPPQWPQDGKIEVNDLSLRYAP 1392

  Fly  1065 D-PQAKYVLKALNFRIRPCEKVGIVGRTGAGKSSLINALFR-LSYNEGIITIDERDTADMGLFDL 1127
            : |:   |:|.::|.:....|:|||||||||||::|.|||| |....|.|.||..|.:.:.|..|
Yeast  1393 NLPR---VIKNVSFSVDAQSKIGIVGRTGAGKSTIITALFRFLEPETGHIKIDNIDISGVDLQRL 1454

  Fly  1128 RSKISIIPQEPVLFSGSMRYNLDPFEEYNDAKLWDALEEVKLKPLISE--LPNG----------- 1179
            |..|:||||:|.||||:::.||||::|::|.::::||:.|.   ||||  |..|
Yeast  1455 RRSITIIPQDPTLFSGTIKTNLDPYDEFSDRQIFEALKRVN---LISEEQLQQGATRETSNEASS 1516

  Fly  1180 -----------LQSKISEGGSNFSVGQRQLVCLARAILRENRVLVMDEATANVDPQTDALIQATI 1233
                       |.|:|||||||.|.|||||:||||::||..:::::|||||::|..:||.||.||
Yeast  1517 TNSENVNKFLDLSSEISEGGSNLSQGQRQLMCLARSLLRSPKIILLDEATASIDYSSDAKIQETI 1581

  Fly  1234 RSKFRDCTVLTIAHRLNTIMDSDRVLVMDAGHLVEFGSPYELLTSSESKIFHGMVMETGQNTFDS 1298
            |.:|:..|:|||||||.:::|.|::||||||.:.|:..||.||.:.:| .|:.|...:|:  .|.
Yeast  1582 RKEFQGSTILTIAHRLRSVIDYDKILVMDAGEVKEYDHPYSLLLNKQS-AFYSMCEHSGE--LDI 1643

  Fly  1299 LLKVAEKAHLESMKPKSE 1316
            |:::|:||.:|.:..|.:
Yeast  1644 LIELAKKAFVEKLNSKKD 1661

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mrp4	NP_650086.2	CFTR_protein	12..1277	CDD:273530	435/1407 (31%)
		ABC_membrane	93..359	CDD:294371	69/307 (22%)
		ABCC_MRP_domain1	422..622	CDD:213217	87/217 (40%)
		ABC_membrane	735..978	CDD:294371	71/286 (25%)
		ABCC_MRP_domain2	1051..1272	CDD:213211	115/246 (47%)
YBT1	NP_013052.1	MRP_assoc_pro	<353..1639	CDD:188098	425/1341 (32%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157344658
Domainoid	1	1.000	64	1.000	Domainoid score	I2425
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100026
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.640