DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mrp4 and cftr

DIOPT Version :9

Sequence 1:	NP_650086.2	Gene:	Mrp4 / 41387	FlyBaseID:	FBgn0263316	Length:	1316	Species:	Drosophila melanogaster
Sequence 2:	NP_001038348.1	Gene:	cftr / 559080	ZFINID:	ZDB-GENE-050517-20	Length:	1485	Species:	Danio rerio

Alignment Length:	1506	Identity:	441/1506 - (29%)
Similarity:	726/1506 - (48%)	Gaps:	241/1506 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPRERSSPLSSLMFCFALPVLFKGRKKTLEQKDLYRALKEHKSDSLGDRLCAAWDEQVAK-N 72
            :..:|.|.::.||...|.:..|::.||.|:.|...|:|:|..:..:|.|.:||...||.:||. .
Zfish     1 MQRSPVEDANCLSRYFFWWTNPIMRKGFKEKLRPSDVYQAPSQDAADILAERLEKEWDREVASGK 65

  Fly    73 ETPRLGRALTK-------VFGFHLFITGVFLLAQEFLTKVTQPICLIGVMAYF-AGNDPDRSKAQ 129
            :.|.|.||:.:       :|||.|:|...        ||..||..|..::|.| ..::|:|:...
Zfish    66 KKPSLLRAMARCYIKPFLLFGFLLYIGEA--------TKTVQPQLLGRIIASFDPAHEPERANGY 122

  Fly   130 LWAAGLIAGSVFSV--CIGHPYMLGLLHLGMKMRIALSSLIYRKALRLSRTALGDTTVGQVVNLL 192
            ..|.||  |.:|:.  .:..|.|.||.||||::||||.|:||:|.|:||...|...:.||:|:|:
Zfish   123 FLAFGL--GLLFTARFLLLQPAMFGLHHLGMQIRIALFSIIYKKTLKLSSRVLDKISTGQLVSLM 185

  Fly   193 SNDVGRFDLVLINVHYLWIAPLELIAVTYLMYLEIGISSMFGVAIMLLFLPFQSYLGKRTSVLRL 257
            |.::|:||..|...|::||:||:.|..|.|::..|.::|...:|.:.|....|::|..:....:.
Zfish   186 SANLGKFDQSLGMAHFIWISPLQCILCTGLIWELIDVNSFCALAAISLLGVLQAFLSHKMGPYKA 250

  Fly   258 RTALRTDERVRMMNEIISGIQVIKMYAWEKPFGKVVEMTRFNEMLCIKQVNYIRGILISFAMFLS 322
            :..|.|::|:.:.:||:..:..:|.|.||:....:::..|.:|:...:::..:| ...|.|.|.|
Zfish   251 QKVLLTNKRLALTSEIMENLHSVKAYGWEEIMETLIKNIRQDEVKLTRKIGSLR-YFYSSAYFFS 314

  Fly   323 RIFTSSSLIAFVLLGNILNAEKAFFVTAYYNILRRSVTMFFPQGISEFAELLVSVRRLEAFMHRP 387
            .||...:.:....|...:|..:.|...:|..:||.:||...|..|..:.:.:..:.::|.|:.:.
Zfish   315 AIFVIVAAVVPHALSRGINLRRIFTTLSYCMVLRMTVTRQLPGSIQMWYDTMRLIWKIEEFLSKE 379

  Fly   388 ETKVRD------------------------KSKVKNANQKAESPNGDSPKGNGIPENLIEFSQFQ 428
            |.|:.:                        ..::|..|:.....|||:.         :.|:...
Zfish   380 EYKLMEYDLSITELELQDVTASWDEGPGELLERIKQENKANGHHNGDAG---------LFFTNLY 435

  Fly   429 ARWESHSLEPTLEDINLQLGRRKLVAVIGPVGAGKSSLIQAILGELPGESGTLRINGSYSYAAQE 493
                   :.|.|:||:|:|.:.:::||.|.:|:|||||:..|||||...||.:|.:|..||::|.
Zfish   436 -------VAPVLKDISLKLKKGEMLAVTGSMGSGKSSLLMTILGELVPSSGKIRHSGRISYSSQT 493

  Fly   494 PWLFTGTVRQNILFGLDWDKHRYRTVVKKCALERDFELLPFGDKTIVGERGASLSGGQKARISLA 558
            .|:..||:|.||||||.:|::||::|||.|.||.|...||..|||.:.|.|.:||||||||::||
Zfish   494 AWIMPGTIRDNILFGLTYDEYRYKSVVKACQLEEDLAALPEKDKTPMAEGGLNLSGGQKARVALA 558

  Fly   559 RAVYRRADIYLLDDPLSAVDTHVGRHLFDQCMRGYLRSELVILVTHQLQFLEQADLIVIMDKGRI 623
            |||||.||:||||.|.:.:|....:.:||:|:...:.|:..||||::::.|::||.|:::..|..
Zfish   559 RAVYRDADLYLLDAPFTHLDIATEKEIFDKCLCKLMASKTRILVTNKIEHLKRADKILLLHNGES 623

  Fly   624 SAMGTYSSMKRSGLDFAQLLTA----PNKDAE-------------DLDEIDGAGGDGLDLLNVP- 670
            ...||:..::....||:.||..    .|..||             .:||..|...:......||
Zfish   624 FFYGTFPELQSERPDFSSLLLGLEAYDNISAERRCSILTETLHRVSVDESAGMQPERSAFRQVPP 688

  Fly   671 ------------------SLSRRGSKNSKP----------------------------------- 682
                              .::|:.|....|
Zfish   689 TKPMYIDERKASVIVNPLGVARKASFIQVPEEEVRRTLPDRKFSLVPENELVDESFMGSDVYHNH 753

  Fly   683 -----STRNNSFTSLSSMAESMAQEASLQ------------------------------------ 706
                 ..|..|..:..:.|:...:...||
Zfish   754 GVHMAGQRRQSVLAFMTNAQGQGRREHLQSSFRRRLSVVPQSELASELDIYTRRLSDSTYDMTGI 818

  Fly   707 MQETRVEGKI--------------GLGLYKEYLTSGSSWFMIFFMVFLCLATQILCSAADYFL-- 755
            ::|..:|..:              ....|..|:::..|...:...:....|.:|..|.|..||
Zfish   819 LEEENIEACLTDEIDEIEETFETTKWNTYVRYVSNNKSLLYVLIFILFIAAIEIAGSVAGIFLIT 883

  Fly   756 -SYWVDK--------------NVDGQT-DINTDPQDMYY----FAALNVAVVVFTIVRTMLFYKM 800
             ..|.::              :..||| .|...|...||    :.|.:.:::.....|.:.|...
Zfish   884 DELWREEHQRSEPNMTKHSNASSSGQTYAITVTPTSSYYILYIYVATSESLLAMGFFRGLPFVHT 948

  Fly   801 AMRSSTQLHNAMYQGITRAAMYFFNTNPSGRILNRFSKDLGQLDEVLPSVMLDVVQLFLTLLGII 865
            .:..|.:||..|...:..|.|...||..:|||:|||:||:..:|::||.:|.|.|||.:.::|.|
Zfish   949 TITISKKLHQKMLHAVLSAPMSVLNTMKTGRIMNRFTKDMATIDDMLPLLMFDFVQLTVVVVGCI 1013

  Fly   866 VVICITNPYYLILTLALAIIFYYIREFYLKTSRDVKRLEAVARSPIYSHLSATITGLPTIRALGA 930
            :|:.|..||..:....|||||..:|:::|:|.:.:|:||..|||||:|||..::.||.||||...
Zfish  1014 LVVSIVRPYIFLAATPLAIIFIVMRKYFLRTGQQLKQLETEARSPIFSHLIMSLKGLWTIRAFER 1078

  Fly   931 QKELIAEFDNLQDLHSSGYYTFLATNRAFGYYLDCFCTLYIVIIILNYFI---NPPQSPGEVGLA 992
            |....|.|....:.|::.::.:|:|.|.|.:..|   .|::....|..:|   .....|||:|:.
Zfish  1079 QAYFEALFHKTLNTHTATWFLYLSTLRWFLFRAD---ILFVFFFTLAAWIAVGTNQDKPGEIGII 1140

  Fly   993 ITQAMGMTGMVQWAMRQSAELENTMTAVERVVEYDEI-----EPEGEFDS-----REGKKPSPSW 1047
            |..||.:.|..||.:..|..::..|.:|:||.::.::     :|:...||     ....:...||
Zfish  1141 ICLAMLILGTFQWCVATSIAVDGMMRSVDRVFKFIDLPSETPKPDKGKDSDLIIENVDAQADSSW 1205

  Fly  1048 PEKGEIIAEDLCLRYFPDPQAKYVLKALNFRIRPCEKVGIVGRTGAGKSSLINALFRLSYNEGII 1112
            |.:|:|...:|.::|.....|  |||.|:|.....::|||:||||:|||||.|||.:|.|.:|.|
Zfish  1206 PHRGQIEVRNLTVKYTEAGHA--VLKNLSFSAEGRQRVGILGRTGSGKSSLFNALLKLVYTDGEI 1268

  Fly  1113 TIDERDTADMGLFDLRSKISIIPQEPVLFSGSMRYNLDPFEEYNDAKLWDALEEVKLKPLISELP 1177
            :||..:...|.|...|....::||:..:|:|.:|.||||:..::|.:||...|||.||.:|.:.|
Zfish  1269 SIDGVNWNKMPLQKWRKAFGVVPQKVFIFTGPLRMNLDPYGCHSDEELWRVAEEVGLKTVIEQFP 1333

  Fly  1178 NGLQSKISEGGSNFSVGQRQLVCLARAILRENRVLVMDEATANVDPQTDALIQATIRSKFRDCTV 1242
            :.|..::..||...|.|.:||:||||:||...|:|::||.:|::||.|..:::.|:|..|..||:
Zfish  1334 DKLDFQLEYGGYVLSNGHKQLICLARSILSGARILLLDEPSAHLDPVTIKVLKKTLRQSFSTCTI 1398

  Fly  1243 LTIAHRLNTIMDSDRVLVMDAGHLVEFGSPYELLTSSES-----------KIF--HGMVMETGQN 1294
            |...|::..:::....|:||.|.:..:.|..:||..:..           |:|  ....|.|.|:
Zfish  1399 LLSEHKVEPLLECQSFLMMDKGQVKTYDSIQKLLNETSHLKQAISPAERLKLFPRRNSSMRTPQS 1463

  Fly  1295 TFDSLLKVAEK 1305
            ...|:.:..::
Zfish  1464 KLSSVTQTLQE 1474

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mrp4	NP_650086.2	CFTR_protein	12..1277	CDD:273530	434/1460 (30%)
		ABC_membrane	93..359	CDD:294371	81/268 (30%)
		ABCC_MRP_domain1	422..622	CDD:213217	90/199 (45%)
		ABC_membrane	735..978	CDD:294371	83/264 (31%)
		ABCC_MRP_domain2	1051..1272	CDD:213211	85/220 (39%)
cftr	NP_001038348.1	AAA	1..1485	CDD:333001	441/1506 (29%)
		Disordered R region. /evidence=ECO:0000250\|UniProtKB:P13569	653..826		16/172 (9%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1452..1485		4/23 (17%)
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P13569	1483..1485
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170586106
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	5	4.850