DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mrp4 and abcc2

DIOPT Version :9

Sequence 1:	NP_650086.2	Gene:	Mrp4 / 41387	FlyBaseID:	FBgn0263316	Length:	1316	Species:	Drosophila melanogaster
Sequence 2:	NP_956883.2	Gene:	abcc2 / 393561	ZFINID:	ZDB-GENE-040426-1523	Length:	1567	Species:	Danio rerio

Alignment Length:	1411	Identity:	489/1411 - (34%)
Similarity:	764/1411 - (54%)	Gaps:	174/1411 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 ADELPE-------NPRERSSPLSSLMFCFALPVLFKGRKKTLEQKDLYRALKEHKSDSLGDRLCA 63
            ||..||       ||..:::.||.:.|.:...:::||.|:.|.|:|::..   :|:||. ..:|.
Zfish   194 ADVSPEAKKVAETNPEAKATFLSRITFNWFNSMVWKGFKRPLVQEDMWDL---NKNDST-HFICQ 254

  Fly    64 AWDEQVAK----------------------------------------NETPR------------ 76
            .:::.:||                                        .||.:
Zfish   255 TFEDVMAKELKKARNNLQKKKSKKRKTVDAEGQNGLAKGVSQDVLVMETETEKESKKKKKKKQKD 319

  Fly    77 ---------LGRALTKVFGFHLFITGVFLLAQEFLTKVTQPICLIGVMAYFAGNDPDRSKAQLWA 132
                     |...:.|.|...|..:.:|.|.|:.|: ...|..|..::|:....|.......|:|
Zfish   320 SDSDYPQSWLVTTIVKSFKGVLLESALFKLLQDLLS-FASPQLLKLMIAFTQNKDSYAWTGYLYA 383

  Fly   133 AGLIAGSVFSVCIGHPYMLGLLHLGMKMRIALSSLIYRKALRLSRTALGDTTVGQVVNLLSNDVG 197
            ..|:..:.....:...|......||||:|.|:.:.:|:|||.:|..:..::|.|::|||:|.|..
Zfish   384 VLLVLVAFVQSVVLQQYFQRCFILGMKVRTAIMAAVYKKALVVSNDSRKESTAGEIVNLMSADAQ 448

  Fly   198 RFDLVLINVHYLWIAPLELIAVTYLMYLEIGISSMFGVAIMLLFLPFQSYLGKRTSVLRLRTALR 262
            ||:.|...:|.||..||::......:::|:|.|.:.|:.:|:|.:|...:|..::...:::....
Zfish   449 RFNDVTNFIHLLWSCPLQIALSIAFLWIELGPSVLSGLLVMVLMVPINGWLATKSRQFQMQNMKF 513

  Fly   263 TDERVRMMNEIISGIQVIKMYAWEKPFGKVVEMTRFNEMLCIKQVNYIRGI-------------L 314
            .|.|::::|::::||:::|.||||..|...|:..|..|:..:::..|:..:             |
Zfish   514 KDSRMKIVNDLLNGIKILKYYAWETSFEAQVQEIREKELKVMRKFAYLSSVSTFIFSCAPALVSL 578

  Fly   315 ISFAMFLSRIFTSSSLIAFVLLGNILNAEKAFFVTAYYNILRRSVTMFFPQGISEFAELLVSVRR 379
            .:||:|:|           |...|||:|||||...:.:||||..:.| .||.||...:..||.:|
Zfish   579 ATFAVFVS-----------VSPDNILDAEKAFTSISLFNILRFPLAM-LPQLISIMVQTSVSKKR 631

  Fly   380 LEAFMHRPE---TKVRDKSKVKNANQKAESPNGDSPKGNGIPENLIEFSQFQARWESHSLEPTLE 441
            ||.|:...:   |.|...|.:..|                     :..:.....|| ...||.|:
Zfish   632 LEKFLSGDDLDTTTVTHDSSITAA---------------------VSMTNGTYAWE-RDTEPVLK 674

  Fly   442 DINLQLGRRKLVAVIGPVGAGKSSLIQAILGELPGESGTLRINGSYSYAAQEPWLFTGTVRQNIL 506
            .::|.:...:||||:|.||:||:||:.|:||||....|.:.||||.:|..|:.|:...|::.|||
Zfish   675 RVSLDIKPGRLVAVVGAVGSGKTSLVSALLGELHSIKGNININGSVAYVPQQAWIQNATLKDNIL 739

  Fly   507 FGLDWDKHRYRTVVKKCALERDFELLPFGDKTIVGERGASLSGGQKARISLARAVYRRADIYLLD 571
            ||...|:.||::|:|.|||..|.:|||..|:|.:||:|.:||||||.|:|||||||..||:||||
Zfish   740 FGSSVDEERYQSVIKACALGPDLDLLPGRDQTEIGEKGINLSGGQKQRVSLARAVYSSADVYLLD 804

  Fly   572 DPLSAVDTHVGRHLFDQCM--RGYLRSELVILVTHQLQFLEQADLIVIMDKGRISAMGTYSSMKR 634
            |||||||:|||:|||::.:  .|.||.:..||:||.:.||...|.||::..|.:|.:|:|.|::.
Zfish   805 DPLSAVDSHVGKHLFEKVIGPNGLLRDKTRILITHGISFLPYVDEIVVLVHGVVSEIGSYESLRA 869

  Fly   635 SGLDFAQLLTAPNKDAEDLD--------------EID--------GAGGDGLDLLNVPSLSRRGS 677
            |...|::.|....||..:.|              ||:        .|.|...|::: .:|.|..|
Zfish   870 SKGAFSEFLETYGKDESNKDNDKKAAAAQTPVYEEIETLPEGLETQADGSPEDIVS-STLKRENS 933

  Fly   678 ---------KNSKPSTRNNSFTSLSSMAESMAQEASLQMQETRVE-GKIGLGLYKEYLTSGSSWF 732
                     :|.....|.||  ||.:..:...::....:::..:| |::...:|.:||::...|:
Zfish   934 LRHSQRHSKRNGSVKVRKNS--SLRAQKDPEDKKGQRLIEKEMMETGRVKFSVYLQYLSAMGWWY 996

  Fly   733 MIFFMVFLCLATQILCSAADYFLSYWVDKNVD--GQTDIN--TDPQDMYYFAALNVAVVVFTIVR 793
            :.|..||..: ..:.....:.:||.|.|.:::  .||..|  .|.: :..|.||.:|........
Zfish   997 VGFSFVFYFI-QNVAVIGQNLWLSDWTDDSIEYFNQTYPNHIRDTR-IGVFGALGLAQGFLVFFG 1059

  Fly   794 TMLFYKMAMRSSTQLHNAMYQGITRAAMYFFNTNPSGRILNRFSKDLGQLDEVLPSVMLDVVQLF 858
            |:|....::.:|..||.::...|.:..|.||:|.|||||:|||:||:..:||::|......:...
Zfish  1060 TILLADGSISASRTLHTSLLTNILKVPMMFFDTTPSGRIVNRFAKDIFTVDEMIPMSFRSWILCL 1124

  Fly   859 LTLLGIIVVICITNPYYLILTLALAIIFYYIREFYLKTSRDVKRLEAVARSPIYSHLSATITGLP 923
            |.:||.:.|||:..|.:..:.:.:|:::|:::.||:.|||.::||::|:|||||||...|::||.
Zfish  1125 LGVLGTLFVICLATPIFTAVVVPMAVVYYFVQRFYVATSRQLRRLDSVSRSPIYSHFGETVSGLS 1189

  Fly   924 TIRALGAQKELIAEFDNLQDLHSSGYYTFLATNRAFGYYLDCFCTLYIVIIILNYFINPPQ-SPG 987
            .|||.|.|...:...::..|.:....|.::.:||.....|:....|.:....|...|:... :.|
Zfish  1190 VIRAYGHQDRFLKRNEHTIDQNLKSVYPWIVSNRWLAMRLESLGNLVVFFAALFAVISRDSLNSG 1254

  Fly   988 EVGLAITQAMGMTGMVQWAMRQSAELENTMTAVERVVEYDEIEPEGEFDSREGKKPSPSWPEKGE 1052
            .|||:|:.|:.:|..:.|.:|.::|||..:.|||||.||.||:.|..:.:  ..:|...||..|.
Zfish  1255 LVGLSISYALNVTQTLNWLVRMTSELETNIVAVERVREYAEIQNEAPWVT--SVRPPDDWPSAGN 1317

  Fly  1053 IIAEDLCLRYFPDPQAKYVLKALNFRIRPCEKVGIVGRTGAGKSSLINALFRL-SYNEGIITIDE 1116
            |..||..:||  .|:.:.||..:...|:..||:|||||||||||||.|.|||: ...:|.|.||:
Zfish  1318 IRFEDYKVRY--RPELELVLHGVTCDIQSTEKIGIVGRTGAGKSSLTNCLFRIVEAADGRILIDD 1380

  Fly  1117 RDTADMGLFDLRSKISIIPQEPVLFSGSMRYNLDPFEEYNDAKLWDALEEVKLKPLISELPNGLQ 1181
            .|.|.:||.||||:::||||:||||||::|.|||||:.::||::|..||...||..:..||.||:
Zfish  1381 IDIATLGLHDLRSRLTIIPQDPVLFSGTLRMNLDPFQTFSDAEIWSVLELAHLKEYVRGLPTGLE 1445

  Fly  1182 SKISEGGSNFSVGQRQLVCLARAILRENRVLVMDEATANVDPQTDALIQATIRSKFRDCTVLTIA 1246
            .::||||.|.|:|||||:|||||:||::|:|::|||||.||.:||.|||:|||.:|..|||||||
Zfish  1446 HEVSEGGENLSLGQRQLLCLARALLRKSRILILDEATAAVDLETDDLIQSTIRREFSHCTVLTIA 1510

  Fly  1247 HRLNTIMDSDRVLVMDAGHLVEFGSPYELLTSSESKIFHGMVMETG 1292
            ||||||:||.||:|:|:|.:|||.||.|||  |:...|..|..:.|
Zfish  1511 HRLNTILDSSRVMVLDSGKIVEFDSPNELL--SKPGHFSSMAEDAG 1554

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mrp4	NP_650086.2	CFTR_protein	12..1277	CDD:273530	480/1381 (35%)
		ABC_membrane	93..359	CDD:294371	79/278 (28%)
		ABCC_MRP_domain1	422..622	CDD:213217	98/201 (49%)
		ABC_membrane	735..978	CDD:294371	77/246 (31%)
		ABCC_MRP_domain2	1051..1272	CDD:213211	127/221 (57%)
abcc2	NP_956883.2	MRP_assoc_pro	10..1555	CDD:188098	489/1411 (35%)
		ABC_membrane	346..612	CDD:294371	79/277 (29%)
		ABCC_MRP_domain1	656..856	CDD:213217	98/200 (49%)
		ABC_membrane	1001..1270	CDD:279056	84/270 (31%)
		ABCC_MRP_domain2	1316..1536	CDD:213211	127/221 (57%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170586123
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG51855
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100026
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	6	5.660