Protein Alignment Mrp4 and Cftr

DIOPT Version :10

• Sequence 1: NP_650086.2
• Gene: Mrp4 / 41387
• FlyBaseID: FBgn0263316
• Length: 1316
• Species: Drosophila melanogaster
• Sequence 2: NP_066388.1
• Gene: Cftr / 12638
• MGIID: 88388
• Length: 1476
• Species: Mus musculus
• Alignment Length: 1486
• Identity: 419/1486 - (28%)
• Similarity: 726/1486 - (48%)
• Gaps: 205/1486 - (13%)

  Fly     9 LPENPRERSSPLSSLMFCFALPVLFKGRKKTLEQKDLYRALKEHKSDSLGDRLCAAWDEQVAKNE 73
            :.::|.|::|.:|.|.|.:..|:|.||.:..||..|:|:|.....:|.|.::|...||.:.|..:
Mouse     1 MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDREQASKK 65

  Fly    74 TPRLGRALTKVFGFHLFITGVFLLAQEFLTKVTQPICLIGVMA-YFAGNDPDRSKAQLWAAGLIA 137
            .|:|..||.:.|.:.....|:.|...| :||..||:.|..::| |...|..:||.|.....||..
Mouse    66 NPQLIHALRRCFFWRFLFYGILLYLGE-VTKAVQPVLLGRIIASYDPENKVERSIAIYLGIGLCL 129

  Fly   138 GSVFSVCIGHPYMLGLLHLGMKMRIALSSLIYRKALRLSRTALGDTTVGQVVNLLSNDVGRFDLV 202
            ..:....:.||.:.||..:||:||.|:.||||:|.|:||...|...::||:|:||||::.:||..
Mouse   130 LFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEG 194

  Fly   203 LINVHYLWIAPLELIAVTYLMYLEIGISSMFGVAIMLLFLPFQSYLGKRTSVLRLRTALRTDERV 267
            |...|::|||||::..:..|::..:..|:..|:.::::.:.||:.|||.....|.:.|.:.:||:
Mouse   195 LALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINERL 259

  Fly   268 RMMNEIISGIQVIKMYAWEKPFGKVVEMTRFNEMLCIKQVNYIRGILISFAMFLSRIF-TSSSLI 331
            .:.:|||..|..:|.|.||....|::|..|..|:...::..|:| ...|.|.|.|..| ...|::
Mouse   260 VITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMR-FFTSSAFFFSGFFVVFLSVL 323

  Fly   332 AFVLLGNILNAEKAFFVTAYYNILRRSVTMFFPQGISEFAELLVSVRRLEAFMHRPETKVRDKSK 396
            .:.::..|: ..|.|...::..:||.|||..||..:..:.:....:|:::.|:.:.|.||.:.:.
Mouse   324 PYTVINGIV-LRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYKVLEYNL 387

  Fly   397 ------VKNAN-----------QKAESPNGDSPKGNGIPENLIEFSQFQARWESHSL---EPTLE 441
                  ::|..           :|.:..|||  :.:...||.:.|        ||..   .|.|:
Mouse   388 MTTGIIMENVTAFWEEGFGELLEKVQQSNGD--RKHSSDENNVSF--------SHLCLVGNPVLK 442

  Fly   442 DINLQLGRRKLVAVIGPVGAGKSSLIQAILGELPGESGTLRINGSYSYAAQEPWLFTGTVRQNIL 506
            :|||.:.:.:::|:.|..|:||:||:..|||||....|.::.:|..|:.:|..|:..||:::||:
Mouse   443 NINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENII 507

  Fly   507 FGLDWDKHRYRTVVKKCALERDFELLPFGDKTIVGERGASLSGGQKARISLARAVYRRADIYLLD 571
            ||:.:|::||::|||.|.|::|.......|.|::||.|.:|||||:||||||||||:.||:||||
Mouse   508 FGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLD 572

  Fly   572 DPLSAVDTHVGRHLFDQCMRGYLRSELVILVTHQLQFLEQADLIVIMDKGRISAMGTYSSMKRSG 636
            .|...:|......:|:.|:...:.::..||||.:::.|.:||.|:|:.:|.....||:|.::...
Mouse   573 SPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLR 637

  Fly   637 LDFAQLL------------------------------TAP------------------------- 646
            .||:..|                              :||
Mouse   638 PDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTGEVGEKRKNSILNS 702

  Fly   647 -----------------NKDAEDLDE------IDGAGGDG----------------------LDL 666
                             :.:::||.|      .|...|:.                      |||
Mouse   703 FSSVRKISIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALPRSNMIATGPTFPGRRRQSVLDL 767

  Fly   667 LN-VPSLSRRGSKNSKPSTRNNSFTSLSSMAE------SMAQEASLQMQE--------------- 709
            :. .|:......:.::.|.|..|.....|:.|      .::|:::|.:.|
Mouse   768 MTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITEEINEEDLKECFLDDV 832

  Fly   710 TRVEGKIGLGLYKEYLTSGSSWFMIFFMVFLCLATQILCSAADYFLSYWVDKN-----VDGQTDI 769
            .::........|..|.|......::.....|....::   ||..|: .|:.||     .:..|.|
Mouse   833 IKIPPVTTWNTYLRYFTLHKGLLLVLIWCVLVFLVEV---AASLFV-LWLLKNNPVNSGNNGTKI 893

  Fly   770 NTD-------PQDMYYFAALNVAV----VVFTIVRTMLFYKMAMRSSTQLHNAMYQGITRAAMYF 823
            :..       ....||...:.|.|    :..::.|.:......:.:|..||..|...|..|.|..
Mouse   894 SNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMST 958

  Fly   824 FNTNPSGRILNRFSKDLGQLDEVLPSVMLDVVQLFLTLLGIIVVICITNPYYLILTLALAIIFYY 888
            .:...:|.||||||||:..||:.||..:.|.:||...::|.|:|:....||..:.|:...::|..
Mouse   959 ISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFIL 1023

  Fly   889 IREFYLKTSRDVKRLEAVARSPIYSHLSATITGLPTIRALGAQKELIAEFDNLQDLHSSGYYTFL 953
            :|.::|.|::.:|:||:..||||::||..::.||.|:||...|......|....:||::.::.:|
Mouse  1024 LRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYL 1088

  Fly   954 ATNRAFGYYLDCFCTLYIVIIILNYFINPPQSPGEVGLAITQAMGMTGMVQWAMRQSAELENTMT 1018
            ||.|.|...:|....|:.:::.....:...:..|..|:.:|.||.:...:|||:..|.:.::.|.
Mouse  1089 ATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLMR 1153

  Fly  1019 AVERVVEYDEIEPEGEFDS-------REGK-----------KPSPSWPEKGEIIAEDLCLRYFPD 1065
            :|.||.::.:|:.|....:       |||.           |.|..||..||::.:||.::|..|
Mouse  1154 SVSRVFKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDD 1218

  Fly  1066 PQAKYVLKALNFRIRPCEKVGIVGRTGAGKSSLINALFRLSYNEGIITIDERDTADMGLFDLRSK 1130
            ..|  ||:.::|.|.|.::||::||||:|||:|::|..|:...:|.|.||......:.|.:.|..
Mouse  1219 GNA--VLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKA 1281

  Fly  1131 ISIIPQEPVLFSGSMRYNLDPFEEYNDAKLWDALEEVKLKPLISELPNGLQSKISEGGSNFSVGQ 1195
            ..:|.|:..:|||:.|.||||..::.|.::|...:||.||.:|.:.|..|...:.:||...|.|.
Mouse  1282 FGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGH 1346

  Fly  1196 RQLVCLARAILRENRVLVMDEATANVDPQTDALIQATIRSKFRDCTVLTIAHRLNTIMDSDRVLV 1260
            :||:||||::|.:.:::::||.:|::||.|..:|:..::..|..|||:...||:..::|..|.||
Mouse  1347 KQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLV 1411

  Fly  1261 MDAGHLVEFGSPYELLTSSESKIFHGMVMETGQNTFDSLLKVAEKAHLESMKPKSE 1316
            ::..::.::.|...||  ||..||...:..:.:      ::..:..|....||:::
Mouse  1412 IEESNVWQYDSLQALL--SEKSIFQQAISSSEK------MRFFQGRHSSKHKPRTQ 1459

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mrp4	NP_650086.2	MRP_assoc_pro	13..1292	CDD:188098	416/1456 (29%)
Cftr	NP_066388.1	CFTR_protein	1..1464	CDD:273530	419/1486 (28%)
		Disordered R region. /evidence=ECO:0000250|UniProtKB:P13569	654..826		19/171 (11%)
		Interaction with GORASP2. /evidence=ECO:0000250|UniProtKB:P13569	1382..1476		20/86 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1446..1476		3/14 (21%)
		PDZ-binding. /evidence=ECO:0000250|UniProtKB:P13569	1474..1476