DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mrp4 and Cftr

DIOPT Version :9

Sequence 1:	NP_650086.2	Gene:	Mrp4 / 41387	FlyBaseID:	FBgn0263316	Length:	1316	Species:	Drosophila melanogaster
Sequence 2:	NP_066388.1	Gene:	Cftr / 12638	MGIID:	88388	Length:	1476	Species:	Mus musculus

Alignment Length:	1486	Identity:	419/1486 - (28%)
Similarity:	726/1486 - (48%)	Gaps:	205/1486 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPRERSSPLSSLMFCFALPVLFKGRKKTLEQKDLYRALKEHKSDSLGDRLCAAWDEQVAKNE 73
            :.::|.|::|.:|.|.|.:..|:|.||.:..||..|:|:|.....:|.|.::|...||.:.|..:
Mouse     1 MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDREQASKK 65

  Fly    74 TPRLGRALTKVFGFHLFITGVFLLAQEFLTKVTQPICLIGVMA-YFAGNDPDRSKAQLWAAGLIA 137
            .|:|..||.:.|.:.....|:.|...| :||..||:.|..::| |...|..:||.|.....||..
Mouse    66 NPQLIHALRRCFFWRFLFYGILLYLGE-VTKAVQPVLLGRIIASYDPENKVERSIAIYLGIGLCL 129

  Fly   138 GSVFSVCIGHPYMLGLLHLGMKMRIALSSLIYRKALRLSRTALGDTTVGQVVNLLSNDVGRFDLV 202
            ..:....:.||.:.||..:||:||.|:.||||:|.|:||...|...::||:|:||||::.:||..
Mouse   130 LFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEG 194

  Fly   203 LINVHYLWIAPLELIAVTYLMYLEIGISSMFGVAIMLLFLPFQSYLGKRTSVLRLRTALRTDERV 267
            |...|::|||||::..:..|::..:..|:..|:.::::.:.||:.|||.....|.:.|.:.:||:
Mouse   195 LALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAILGKMMVKYRDQRAAKINERL 259

  Fly   268 RMMNEIISGIQVIKMYAWEKPFGKVVEMTRFNEMLCIKQVNYIRGILISFAMFLSRIF-TSSSLI 331
            .:.:|||..|..:|.|.||....|::|..|..|:...::..|:| ...|.|.|.|..| ...|::
Mouse   260 VITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKAAYMR-FFTSSAFFFSGFFVVFLSVL 323

  Fly   332 AFVLLGNILNAEKAFFVTAYYNILRRSVTMFFPQGISEFAELLVSVRRLEAFMHRPETKVRDKSK 396
            .:.::..|: ..|.|...::..:||.|||..||..:..:.:....:|:::.|:.:.|.||.:.:.
Mouse   324 PYTVINGIV-LRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSFGMIRKIQDFLQKQEYKVLEYNL 387

  Fly   397 ------VKNAN-----------QKAESPNGDSPKGNGIPENLIEFSQFQARWESHSL---EPTLE 441
                  ::|..           :|.:..|||  :.:...||.:.|        ||..   .|.|:
Mouse   388 MTTGIIMENVTAFWEEGFGELLEKVQQSNGD--RKHSSDENNVSF--------SHLCLVGNPVLK 442

  Fly   442 DINLQLGRRKLVAVIGPVGAGKSSLIQAILGELPGESGTLRINGSYSYAAQEPWLFTGTVRQNIL 506
            :|||.:.:.:::|:.|..|:||:||:..|||||....|.::.:|..|:.:|..|:..||:::||:
Mouse   443 NINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENII 507

  Fly   507 FGLDWDKHRYRTVVKKCALERDFELLPFGDKTIVGERGASLSGGQKARISLARAVYRRADIYLLD 571
            ||:.:|::||::|||.|.|::|.......|.|::||.|.:|||||:||||||||||:.||:||||
Mouse   508 FGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLD 572

  Fly   572 DPLSAVDTHVGRHLFDQCMRGYLRSELVILVTHQLQFLEQADLIVIMDKGRISAMGTYSSMKRSG 636
            .|...:|......:|:.|:...:.::..||||.:::.|.:||.|:|:.:|.....||:|.::...
Mouse   573 SPFGYLDVFTEEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLR 637

  Fly   637 LDFAQLL------------------------------TAP------------------------- 646
            .||:..|                              :||
Mouse   638 PDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDSSAPWSKPKQSFRQTGEVGEKRKNSILNS 702

  Fly   647 -----------------NKDAEDLDE------IDGAGGDG----------------------LDL 666
                             :.:::||.|      .|...|:.                      |||
Mouse   703 FSSVRKISIVQKTPLCIDGESDDLQEKRLSLVPDSEQGEAALPRSNMIATGPTFPGRRRQSVLDL 767

  Fly   667 LN-VPSLSRRGSKNSKPSTRNNSFTSLSSMAE------SMAQEASLQMQE--------------- 709
            :. .|:......:.::.|.|..|.....|:.|      .::|:::|.:.|
Mouse   768 MTFTPNSGSSNLQRTRTSIRKISLVPQISLNEVDVYSRRLSQDSTLNITEEINEEDLKECFLDDV 832

  Fly   710 TRVEGKIGLGLYKEYLTSGSSWFMIFFMVFLCLATQILCSAADYFLSYWVDKN-----VDGQTDI 769
            .::........|..|.|......::.....|....::   ||..|: .|:.||     .:..|.|
Mouse   833 IKIPPVTTWNTYLRYFTLHKGLLLVLIWCVLVFLVEV---AASLFV-LWLLKNNPVNSGNNGTKI 893

  Fly   770 NTD-------PQDMYYFAALNVAV----VVFTIVRTMLFYKMAMRSSTQLHNAMYQGITRAAMYF 823
            :..       ....||...:.|.|    :..::.|.:......:.:|..||..|...|..|.|..
Mouse   894 SNSSYVVIITSTSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMST 958

  Fly   824 FNTNPSGRILNRFSKDLGQLDEVLPSVMLDVVQLFLTLLGIIVVICITNPYYLILTLALAIIFYY 888
            .:...:|.||||||||:..||:.||..:.|.:||...::|.|:|:....||..:.|:...::|..
Mouse   959 ISKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFIL 1023

  Fly   889 IREFYLKTSRDVKRLEAVARSPIYSHLSATITGLPTIRALGAQKELIAEFDNLQDLHSSGYYTFL 953
            :|.::|.|::.:|:||:..||||::||..::.||.|:||...|......|....:||::.::.:|
Mouse  1024 LRAYFLHTAQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYL 1088

  Fly   954 ATNRAFGYYLDCFCTLYIVIIILNYFINPPQSPGEVGLAITQAMGMTGMVQWAMRQSAELENTMT 1018
            ||.|.|...:|....|:.:::.....:...:..|..|:.:|.||.:...:|||:..|.:.::.|.
Mouse  1089 ATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLMR 1153

  Fly  1019 AVERVVEYDEIEPEGEFDS-------REGK-----------KPSPSWPEKGEIIAEDLCLRYFPD 1065
            :|.||.::.:|:.|....:       |||.           |.|..||..||::.:||.::|..|
Mouse  1154 SVSRVFKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDD 1218

  Fly  1066 PQAKYVLKALNFRIRPCEKVGIVGRTGAGKSSLINALFRLSYNEGIITIDERDTADMGLFDLRSK 1130
            ..|  ||:.::|.|.|.::||::||||:|||:|::|..|:...:|.|.||......:.|.:.|..
Mouse  1219 GNA--VLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKA 1281

  Fly  1131 ISIIPQEPVLFSGSMRYNLDPFEEYNDAKLWDALEEVKLKPLISELPNGLQSKISEGGSNFSVGQ 1195
            ..:|.|:..:|||:.|.||||..::.|.::|...:||.||.:|.:.|..|...:.:||...|.|.
Mouse  1282 FGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLSHGH 1346

  Fly  1196 RQLVCLARAILRENRVLVMDEATANVDPQTDALIQATIRSKFRDCTVLTIAHRLNTIMDSDRVLV 1260
            :||:||||::|.:.:::::||.:|::||.|..:|:..::..|..|||:...||:..::|..|.||
Mouse  1347 KQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAMLDCQRFLV 1411

  Fly  1261 MDAGHLVEFGSPYELLTSSESKIFHGMVMETGQNTFDSLLKVAEKAHLESMKPKSE 1316
            ::..::.::.|...||  ||..||...:..:.:      ::..:..|....||:::
Mouse  1412 IEESNVWQYDSLQALL--SEKSIFQQAISSSEK------MRFFQGRHSSKHKPRTQ 1459

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mrp4	NP_650086.2	CFTR_protein	12..1277	CDD:273530	411/1442 (29%)
		ABC_membrane	93..359	CDD:294371	87/267 (33%)
		ABCC_MRP_domain1	422..622	CDD:213217	79/202 (39%)
		ABC_membrane	735..978	CDD:294371	72/258 (28%)
		ABCC_MRP_domain2	1051..1272	CDD:213211	77/220 (35%)
Cftr	NP_066388.1	CFTR_protein	1..1464	CDD:273530	419/1486 (28%)
		ABC_membrane	85..303	CDD:294371	75/218 (34%)
		ABCC_CFTR1	389..670	CDD:213258	93/290 (32%)
		CFTR_R	639..844	CDD:291094	22/204 (11%)
		Disordered R region. /evidence=ECO:0000250\|UniProtKB:P13569	654..826		19/171 (11%)
		ABC_membrane	861..1142	CDD:279056	79/284 (28%)
		ABCC_CFTR2	1204..1464	CDD:213256	87/266 (33%)
		Interaction with GORASP2. /evidence=ECO:0000250\|UniProtKB:P13569	1382..1476		20/86 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1446..1476		3/14 (21%)
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P13569	1474..1476
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167841713
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.850