DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mrp4 and CFTR

DIOPT Version :9

Sequence 1:	NP_650086.2	Gene:	Mrp4 / 41387	FlyBaseID:	FBgn0263316	Length:	1316	Species:	Drosophila melanogaster
Sequence 2:	NP_000483.3	Gene:	CFTR / 1080	HGNCID:	1884	Length:	1480	Species:	Homo sapiens

Alignment Length:	1500	Identity:	434/1500 - (28%)
Similarity:	735/1500 - (49%)	Gaps:	231/1500 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly     9 LPENPRERSSPLSSLMFCFALPVLFKGRKKTLEQKDLYRALKEHKSDSLGDRLCAAWDEQVAKNE 73
            :..:|.|::|.:|.|.|.:..|:|.||.::.||..|:|:......:|:|.::|...||.::|..:
Human     1 MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKK 65

  Fly    74 TPRLGRALTKVFGFHLFITGVFLLAQEFLTKVTQPICLIGVMA-YFAGNDPDRSKAQLWAAGLIA 137
            .|:|..||.:.|.:.....|:||...| :||..||:.|..::| |...|..:||.|.....||..
Human    66 NPKLINALRRCFFWRFMFYGIFLYLGE-VTKAVQPLLLGRIIASYDPDNKEERSIAIYLGIGLCL 129

  Fly   138 GSVFSVCIGHPYMLGLLHLGMKMRIALSSLIYRKALRLSRTALGDTTVGQVVNLLSNDVGRFDLV 202
            ..:....:.||.:.||.|:||:||||:.||||:|.|:||...|...::||:|:||||::.:||..
Human   130 LFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEG 194

  Fly   203 LINVHYLWIAPLELIAVTYLMYLEIGISSMFGVAIMLLFLPFQSYLGKRTSVLRLRTALRTDERV 267
            |...|::|||||::..:..|::..:..|:..|:..:::...||:.||:.....|.:.|.:..||:
Human   195 LALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAGLGRMMMKYRDQRAGKISERL 259

  Fly   268 RMMNEIISGIQVIKMYAWEKPFGKVVEMTRFNEMLCIKQVNYIRGILISFAMFLSRIF-TSSSLI 331
            .:.:|:|..||.:|.|.||:...|::|..|..|:...::..|:| ...|.|.|.|..| ...|::
Human   260 VITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAYVR-YFNSSAFFFSGFFVVFLSVL 323

  Fly   332 AFVLLGNILNAEKAFFVTAYYNILRRSVTMFFPQGISEFAELLVSVRRLEAFMHRPETKVRD--- 393
            .:.|:..|: ..|.|...::..:||.:||..||..:..:.:.|.::.:::.|:.:.|.|..:
Human   324 PYALIKGII-LRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNL 387

  Fly   394 -KSKVKNAN-------------QKAESPNGDSPKGNGIPENLIEFSQFQARWESHSL--EPTLED 442
             .::|...|             :||:..|.:....||  ::.:.||.|       ||  .|.|:|
Human   388 TTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNG--DDSLFFSNF-------SLLGTPVLKD 443

  Fly   443 INLQLGRRKLVAVIGPVGAGKSSLIQAILGELPGESGTLRINGSYSYAAQEPWLFTGTVRQNILF 507
            ||.::.|.:|:||.|..||||:||:..|:|||....|.::.:|..|:.:|..|:..||:::||:|
Human   444 INFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIF 508

  Fly   508 GLDWDKHRYRTVVKKCALERDFELLPFGDKTIVGERGASLSGGQKARISLARAVYRRADIYLLDD 572
            |:.:|::|||:|:|.|.||.|.......|..::||.|.:|||||:||||||||||:.||:||||.
Human   509 GVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDS 573

  Fly   573 PLSAVDTHVGRHLFDQCMRGYLRSELVILVTHQLQFLEQADLIVIMDKGRISAMGTYSSMKRSGL 637
            |...:|....:.:|:.|:...:.::..||||.:::.|::||.|:|:.:|.....||:|.::....
Human   574 PFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQP 638

  Fly   638 DFA------------------QLLT-----------AP--------------------------- 646
            ||:                  .:||           ||
Human   639 DFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAPVSWTETKKQSFKQTGEFGEKRKNSILN 703

  Fly   647 ------------------NKDAEDLDE---------IDGAGGDGL-------------------D 665
                              |...||.||         .|...|:.:                   .
Human   704 PINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGPTLQARRRQS 768

  Fly   666 LLNVPSLSRRGSKN----SKPSTRNNS------FTSLSSMAESMAQEASLQMQE----------- 709
            :||:.:.|....:|    :..|||..|      .|.|...:..::||..|::.|
Human   769 VLNLMTHSVNQGQNIHRKTTASTRKVSLAPQANLTELDIYSRRLSQETGLEISEEINEEDLKECF 833

  Fly   710 ----TRVEGKIGLGLYKEYLTSGSSWFMIFFMVFLCLATQILCSAADYFLSYWVDKNVDGQTDIN 770
                ..:........|..|:|...|   :.|::..||.. .|...|...:..|:..|...|...|
Human   834 FDDMESIPAVTTWNTYLRYITVHKS---LIFVLIWCLVI-FLAEVAASLVVLWLLGNTPLQDKGN 894

  Fly   771 -------------TDPQDMYYFAALNVAVVVFTIVRTMLFYK------MAMRSSTQLHNAMYQGI 816
                         |.....|.|   .:.|.|...:..|.|::      ..:..|..||:.|...:
Human   895 STHSRNNSYAVIITSTSSYYVF---YIYVGVADTLLAMGFFRGLPLVHTLITVSKILHHKMLHSV 956

  Fly   817 TRAAMYFFNTNPSGRILNRFSKDLGQLDEVLPSVMLDVVQLFLTLLGIIVVICITNPYYLILTLA 881
            .:|.|...||..:|.||||||||:..||::||..:.|.:||.|.::|.|.|:.:..||..:.|:.
Human   957 LQAPMSTLNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVP 1021

  Fly   882 LAIIFYYIREFYLKTSRDVKRLEAVARSPIYSHLSATITGLPTIRALGAQKELIAEFDNLQDLHS 946
            :.:.|..:|.::|:||:.:|:||:..||||::||..::.||.|:||.|.|......|....:||:
Human  1022 VIVAFIMLRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHT 1086

  Fly   947 SGYYTFLATNRAFGYYLDCFCTLYIVIIILNYFINPPQSPGEVGLAITQAMGMTGMVQWAMRQSA 1011
            :.::.:|:|.|.|...::....::.:.:.....:...:..|.||:.:|.||.:...:|||:..|.
Human  1087 ANWFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSI 1151

  Fly  1012 ELENTMTAVERVVEYDEIEPEGEFDSREGKKPSPS-------------------------WPEKG 1051
            ::::.|.:|.||.::.::..||        ||:.|                         ||..|
Human  1152 DVDSLMRSVSRVFKFIDMPTEG--------KPTKSTKPYKNGQLSKVMIIENSHVKKDDIWPSGG 1208

  Fly  1052 EIIAEDLCLRYFPDPQAKYVLKALNFRIRPCEKVGIVGRTGAGKSSLINALFRLSYNEGIITIDE 1116
            ::..:||..:|.....|  :|:.::|.|.|.::||::||||:|||:|::|..||...||.|.||.
Human  1209 QMTVKDLTAKYTEGGNA--ILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEIQIDG 1271

  Fly  1117 RDTADMGLFDLRSKISIIPQEPVLFSGSMRYNLDPFEEYNDAKLWDALEEVKLKPLISELPNGLQ 1181
            .....:.|...|....:|||:..:|||:.|.||||:|:::|.::|...:||.|:.:|.:.|..|.
Human  1272 VSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLD 1336

  Fly  1182 SKISEGGSNFSVGQRQLVCLARAILRENRVLVMDEATANVDPQTDALIQATIRSKFRDCTVLTIA 1246
            ..:.:||...|.|.:||:||||::|.:.::|::||.:|::||.|..:|:.|::..|.||||:...
Human  1337 FVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCE 1401

  Fly  1247 HRLNTIMDSDRVLVMDAGHLVEFGSPYELLTSSESKIFHGMVMETGQNTFDSLLKVAEKAHLESM 1311
            ||:..:::..:.||::...:.::.|..:||  :|..:|...:..:.        :|....|..|.
Human  1402 HRIEAMLECQQFLVIEENKVRQYDSIQKLL--NERSLFRQAISPSD--------RVKLFPHRNSS 1456

  Fly  1312 KPKSE 1316
            |.||:
Human  1457 KCKSK 1461

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mrp4	NP_650086.2	CFTR_protein	12..1277	CDD:273530	425/1456 (29%)
		ABC_membrane	93..359	CDD:294371	90/267 (34%)
		ABCC_MRP_domain1	422..622	CDD:213217	84/201 (42%)
		ABC_membrane	735..978	CDD:294371	75/261 (29%)
		ABCC_MRP_domain2	1051..1272	CDD:213211	78/220 (35%)
CFTR	NP_000483.3	CFTR_protein	1..1480	CDD:273530	434/1499 (29%)
		ABC_membrane	85..303	CDD:279056	77/218 (35%)
		ABCC_CFTR1	389..670	CDD:213258	99/289 (34%)
		CFTR_R	639..849	CDD:291094	27/209 (13%)
		Disordered R region. /evidence=ECO:0000269\|PubMed:10792060	654..831		25/176 (14%)
		ABC_membrane	866..1147	CDD:279056	82/284 (29%)
		ABCC_CFTR2	1208..1480	CDD:213256	89/265 (34%)
		Interaction with GORASP2. /evidence=ECO:0000269\|PubMed:21884936	1386..1480		21/85 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1452..1480		5/9 (56%)
		PDZ-binding. /evidence=ECO:0000269\|PubMed:11707463, ECO:0000269\|PubMed:16331976	1478..1480
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165151595
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG0054
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D138195at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24223
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	6	5.850