DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pug and ADE3

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_011720.3	Gene:	ADE3 / 853118	SGDID:	S000003436	Length:	946	Species:	Saccharomyces cerevisiae

Alignment Length:	956	Identity:	518/956 - (54%)
Similarity:	658/956 - (68%)	Gaps:	32/956 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly    35 MSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGI 99
            |:| :::.|.|.|:..|..:.||:.::...:..|.|.|.|:|||.|.||..|:|||.|||.|.||
Yeast     1 MAG-QVLDGKACAQQFRSNIANEIKSIQGHVPGFAPNLAIIQVGNRPDSATYVRMKRKAAEEAGI 64

  Fly   100 DAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTV 164
            .|..:.|..|.||.|:|..::.|||||..||||||:||...  :|..|||..|..||||||....
Yeast    65 VANFIHLDESATEFEVLRYVDQLNEDPHTHGIIVQLPLPAH--LDEDRITSRVLAEKDVDGFGPT 127

  Fly   165 NEGRLAIGDLGG------FLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANA 223
            |     ||:|..      ||||||.|.:||:.::.|.|.|:|:||:|||.|||:|.|||||..|:
Yeast   128 N-----IGELNKKNGHPFFLPCTPKGIIELLHKANVTIEGSRSVVIGRSDIVGSPVAELLKSLNS 187

  Fly   224 TVTVCHSKTRNLEEITRSADILVVGIGVAEMVKGSWIKP--------GAVVIDCGINVKPDASKA 280
            |||:.|||||::......|||:||.||..|.|||.|.||        ..||||.|.|...|.||.
Yeast   188 TVTITHSKTRDIASYLHDADIVVVAIGQPEFVKGEWFKPRDGTSSDKKTVVIDVGTNYVADPSKK 252

  Fly   281 SGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKP 345
            ||.|.||||::.||::....:||||||||||||||||:||:.:|.|.:|..:| ...:..||||.
Yeast   253 SGFKCVGDVEFNEAIKYVHLITPVPGGVGPMTVAMLMQNTLIAAKRQMEESSK-PLQIPPLPLKL 316

  Fly   346 QRPVPSDIVIARAQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGM 410
            ..||||||.|:|||:||.|..||:|:|:.:.|:.|||:.|||||..|:|||:.::.|.|::|:|:
Yeast   317 LTPVPSDIDISRAQQPKLINQLAQELGIYSHELELYGHYKAKISPKVIERLQTRQNGKYILVSGI 381

  Fly   411 TPTPLGEGKTTTLMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHL 475
            |||||||||:||.|||||||.||..:..:|.:||||.|||.|:|||||||||:|||||:||||||
Yeast   382 TPTPLGEGKSTTTMGLVQALTAHLGKPAIANVRQPSLGPTLGVKGGAAGGGYSQVIPMDEFNLHL 446

  Fly   476 TGDIHAVSAANNLLAAQLDTRIFHENTQK-DKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTD 539
            ||||||:.||||||||.:|||:|||.||| |...|:||||...|:|||:|...|||.:|||.||:
Yeast   447 TGDIHAIGAANNLLAAAIDTRMFHETTQKNDATFYNRLVPRKNGKRKFTPSMQRRLNRLGIQKTN 511

  Fly   540 PDTLTADEYGPFARLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMA 604
            ||.||.:|...||||:||||||..:||||||||.||.||:||:||||..:|.|.|.|:||||:||
Yeast   512 PDDLTPEEINKFARLNIDPDTITIKRVVDINDRMLRQITIGQAPTEKNHTRVTGFDITVASELMA 576

  Fly   605 VLALSRSLEDMKQRLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHA 669
            :||||:.|.|||:|:..:|||.|....|||.:|:|.||||..||:||::|||||:||||||||||
Yeast   577 ILALSKDLRDMKERIGRVVVAADVNRSPVTVEDVGCTGALTALLRDAIKPNLMQTLEGTPVLVHA 641

  Fly   670 GPFANIAHGCNSIIADEVGLKLVG-------KNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNA 727
            ||||||:.|.:|:|||.|.|||||       :.|:|.|||||...:|.|:|.|||||:||..|||
Yeast   642 GPFANISIGASSVIADRVALKLVGTEPEAKTEAGYVVTEAGFDFTMGGERFFNIKCRSSGLTPNA 706

  Fly   728 MVLVATVRAIKMHGGGAPVTPGAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAH 792
            :||||||||:|.|||...|.||.||...|||||:|.|:||..|:.:.|.|.|.||:||||::|..
Yeast   707 VVLVATVRALKSHGGAPDVKPGQPLPSAYTEENIEFVEKGAANMCKQIANIKQFGVPVVVAINKF 771

  Fly   793 SADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVD 857
            ..||..|...::|||||||||.||.|.|||:||.||:.||.|||:|..|...|..|||:...:.|
Yeast   772 ETDTEGEIAAIRKAALEAGAFEAVTSNHWAEGGKGAIDLAKAVIEASNQPVDFHFLYDVNSSVED 836

  Fly   858 KMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVE 922
            |:..|...|||...:.:.|.|:.|:....:.||||||||::|...|.:.||.:||.|.|||..:.
Yeast   837 KLTTIVQKMYGGAAIDILPEAQRKIDMYKEQGFGNLPICIAKTQYSLSHDATLKGVPTGFTFPIR 901

  Fly   923 DVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYDIDLNTETGEIEGLF 968
            ||.:|.|||::.|:..|:..:|||.|......:::: :.|||:|||
Yeast   902 DVRLSNGAGYLYALAAEIQTIPGLATYAGYMAVEVD-DDGEIDGLF 946

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	149/304 (49%)
		THF_DHG_CYH	40..159	CDD:279147	54/118 (46%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	96/183 (52%)
		FTHFS	350..968	CDD:279592	357/625 (57%)
ADE3	NP_011720.3	FolD	4..303	CDD:223268	150/305 (49%)
ADE3	NP_011720.3	FTHFS	322..946	CDD:396018	356/624 (57%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	693	1.000	Domainoid score	I11
eggNOG	1	0.900	-	-		E1_COG2759
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H55940
Inparanoid	1	1.050	985	1.000	Inparanoid score	I29
Isobase	1	0.950	-	0.875	Normalized mean entropy	S317
OMA	1	1.010	-	-		QHG53867
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	1	1.000	-	-		otm46839
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	1	1.100	-	-	LDO	PTHR48099
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R159
SonicParanoid	1	1.000	-	-		X878
TreeFam	1	0.960	-	-
	15	14.810