DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pug and MIS1

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_009640.1	Gene:	MIS1 / 852378	SGDID:	S000000288	Length:	975	Species:	Saccharomyces cerevisiae

Alignment Length:	972	Identity:	521/972 - (53%)
Similarity:	683/972 - (70%)	Gaps:	31/972 - (3%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 KWPAEKSKVGSGEWTAEPAIKMSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVG 78
            :|...:.||       .|.:..|...|:||..:|:||||:..:|:.|:..:..:|.|.|:|:|||
Yeast    18 RWRIYRLKV-------SPTVHASQYHILSGRKLAQSIREKANDEIQAIKLKHPNFKPTLKIIQVG 75

  Fly    79 GREDSNVYIRMKIKAATEIGIDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPI 143
            .|.||:.|:|||:||:.:..:|....:||..|||||||.||:|:|:|..:||:::|:||  ...:
Yeast    76 ARPDSSTYVRMKLKASKDSNVDCIIEKLPAEITEVELLKKISDINDDDSIHGLLIQLPL--PRHL 138

  Fly   144 DSHRITDAVSPEKDVDGLHTVNEGRLAIGDLGG---FLPCTPWGCLELIRRSGVEIAGARAVVLG 205
            |...||:||..:|||||.|..|.|.||  ..||   |:||||:||::|:..:.|::.|..|||||
Yeast   139 DETTITNAVDFKKDVDGFHRYNAGELA--KKGGKPYFIPCTPYGCMKLLEEAHVKLDGKNAVVLG 201

  Fly   206 RSKIVGTPAAELLKWANATVTVCHSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCG 270
            ||.|||.|.|.|||.||||||||||.|||:.|:...|||::...|:.:.||..|||.||||||.|
Yeast   202 RSSIVGNPIASLLKNANATVTVCHSHTRNIAEVVSQADIVIAACGIPQYVKSDWIKEGAVVIDVG 266

  Fly   271 INVKPDASKASGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAAR-FLERLAKS 334
            ||..||.||.||.|||||||:....:...::|||||||||||||||:.|.:.:|.| |:|  ::.
Yeast   267 INYVPDISKKSGQKLVGDVDFDSVKEKTSYITPVPGGVGPMTVAMLVSNVLLAAKRQFVE--SEK 329

  Fly   335 QWALQTLPLKPQRPVPSDIVIARAQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDK 399
            ...::.|||..:.||||||.|:|||.||.|..:|:|:|:.:.|:.|||:.|||||.::.:||:.:
Yeast   330 LPVIKPLPLHLESPVPSDIDISRAQSPKHIKQVAEELGIHSHELELYGHYKAKISPNIFKRLESR 394

  Fly   400 EVGHYVVVAGMTPTPLGEGKTTTLMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQ 464
            |.|.||:|||:|||||||||:||.|||||||.||..:.::|.:||||.|||.|:|||||||||||
Yeast   395 ENGKYVLVAGITPTPLGEGKSTTTMGLVQALSAHLGKPSIANVRQPSLGPTLGVKGGAAGGGYAQ 459

  Fly   465 VIPMEEFNLHLTGDIHAVSAANNLLAAQLDTRIFHENTQK-DKALYDRLVPAIKGQRKFSPIQLR 528
            ||||:||||||||||||:|||||||||.:|||:|||.||| |...|.||||..||.|||:|...|
Yeast   460 VIPMDEFNLHLTGDIHAISAANNLLAAAIDTRMFHEATQKNDSTFYKRLVPRKKGIRKFTPSMQR 524

  Fly   529 RLQKLGITKTDPDTLTADEYGPFARLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETR 593
            ||::|.|.|.|||.||.:|...||||:|:||||...||||||||.||.||:|::.||||.:|.|.
Yeast   525 RLKRLDIEKEDPDALTPEEVKRFARLNINPDTITIRRVVDINDRMLRQITIGEAATEKGFTRTTG 589

  Fly   594 FSISVASEIMAVLALSRSLEDMKQRLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQ 658
            |.|:||||:||:||||:||.:||:|:..||:..|...||||.:|:|.||||..||:||::|||||
Yeast   590 FDITVASELMAILALSKSLHEMKERIGRMVIGADYDNKPVTVEDIGCTGALTALLRDAIKPNLMQ 654

  Fly   659 SLEGTPVLVHAGPFANIAHGCNSIIADEVGLKLVG--------KN----GFVCTEAGFGSDIGME 711
            :||||||:|||||||||:.|.:|:|||.:.|||||        ||    |:|.|||||...:|.|
Yeast   655 TLEGTPVMVHAGPFANISIGASSVIADLMALKLVGSEKNPLNDKNIHEPGYVVTEAGFDFAMGGE 719

  Fly   712 KFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAPLNKQYTEENLELVQKGLPNLLQHIE 776
            :|.:||||:||..|:|:||||||||:|.|||...|.||..|.|:|||||::.|.||:.||::.||
Yeast   720 RFFDIKCRSSGLVPDAVVLVATVRALKSHGGAPNVKPGQSLPKEYTEENIDFVAKGVSNLVKQIE 784

  Fly   777 NGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQLADAVIKACEQ 841
            |.|.||:||||::|....|:.||.|::|||||.|||..||.|.||.:||.|||:||.||:.|.::
Yeast   785 NIKTFGIPVVVAINRFETDSQAEIEVIKKAALNAGASHAVTSNHWMEGGKGAVELAHAVVDATKE 849

  Fly   842 GNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTG 906
            ...|..|||:...:.||:..|...|||..|:.:||.|::|:......||||||||::|...|.:.
Yeast   850 PKNFNFLYDVNSSIEDKLTSIVQKMYGGAKIEVSPEAQKKIDTYKKQGFGNLPICIAKTQYSLSH 914

  Fly   907 DAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYDIDLNTETGEIEGLF 968
            |..:||.|:|||..:.||..|.|||::.|:..|:..:|||.|......:::: :.|||||||
Yeast   915 DPSLKGVPRGFTFPIRDVRASIGAGYLYALAAEIQTIPGLSTYAGYMAVEVD-DDGEIEGLF 975

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	152/294 (52%)
		THF_DHG_CYH	40..159	CDD:279147	53/118 (45%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	98/172 (57%)
		FTHFS	350..968	CDD:279592	356/630 (57%)
MIS1	NP_009640.1	FolD	37..327	CDD:223268	153/295 (52%)
MIS1	NP_009640.1	FTHFS	345..975	CDD:396018	356/630 (57%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157345530
Domainoid	1	1.000	693	1.000	Domainoid score	I11
eggNOG	1	0.900	-	-		E1_COG2759
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	985	1.000	Inparanoid score	I29
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53867
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	1	1.000	-	-		otm46839
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R159
SonicParanoid	1	1.000	-	-		X878
TreeFam	1	0.960	-	-
	13	12.690