DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pug and Mthfd1

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	XP_006240311.1	Gene:	Mthfd1 / 64300	RGDID:	708531	Length:	941	Species:	Rattus norvegicus

Alignment Length:	924	Identity:	569/924 - (61%)
Similarity:	705/924 - (76%)	Gaps:	1/924 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    46 VAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSI 110
            |::.||..|:.:||.|.:|:..|.|||.|:|||.|:|||:||.:|:|||.||||.|.|::|||:.
  Rat    18 VSRQIRNRLKTQVTQMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAQEIGIKATHIKLPRTS 82

  Fly   111 TEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDLG 175
            ||.|:|..:..||||..|||.|||:|||.:..|::..:.:|::||||||||.::|.|:||.|||.
  Rat    83 TESEVLKYVISLNEDATVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSINAGKLARGDLK 147

  Fly   176 G-FLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSKTRNLEEIT 239
            . |:||||.||||||:.:||:|||..|||:|||||||.|..:||.|.|||||.|||||.:|::..
  Rat   148 DCFIPCTPKGCLELIKETGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTADLDKEV 212

  Fly   240 RSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQVAGHLTPV 304
            ...|||||..|..|||||.||||||||||||||..||.:|.:|.|:||||.|.||.:.|..:|||
  Rat   213 NKGDILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKEKASFITPV 277

  Fly   305 PGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKPKDIAVLAK 369
            |||||||||||||::||.||.|||::....:|.:|...|..:.||||||.|:|:.|||.|..||:
  Rat   278 PGGVGPMTVAMLMQSTVESAQRFLKKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNLAR 342

  Fly   370 EIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGLVQALGAHK 434
            ||||...||.|||..|||:.||.|:|||.:..|.||||.|:|||||||||:||.:|||||||||.
  Rat   343 EIGLLTEEVELYGETKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAHL 407

  Fly   435 LRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAAQLDTRIFH 499
            .:|..|.:||||||||||||||||||||:||||||||||||||||||::|||||:||.:|.||||
  Rat   408 HQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFH 472

  Fly   500 ENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDIDPDTIMWE 564
            |.||.||||::||||::.|.||||.||:|||::|||.||||..||.||...||||||||:||.|:
  Rat   473 ELTQTDKALFNRLVPSVNGVRKFSDIQIRRLRRLGIEKTDPAALTDDEINRFARLDIDPETITWQ 537

  Fly   565 RVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLADMVVAFDKR 629
            ||:|.|||:||.||:||:|||||.:|..:|.|||||||||||||:.|||||:.||..||||..|:
  Rat   538 RVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRARLGKMVVASSKK 602

  Fly   630 GKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADEVGLKLVGK 694
            |:|::.:||||:|||.||:|||::|||||:||||||.||||||||||||.:|||||.:.|||||.
  Rat   603 GEPISCEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGP 667

  Fly   695 NGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAPLNKQYTEE 759
            .|||.||||||:|||||||.|||||.||.:|:.:||||||||:||||||..||.|.||.|.||||
  Rat   668 EGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEE 732

  Fly   760 NLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADG 824
            :|:||:||..||.:.|||.:.||:||||::||...||.||.:|:.:.:.|.|||.||..||||:|
  Rat   733 DLDLVEKGFSNLRKQIENARMFGVPVVVAMNAFKTDTDAELDLIGRLSREHGAFDAVKCTHWAEG 797

  Fly   825 GAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAG 889
            |.||:.||.||.:|.:..:.|:|||||:|.:.||:..||..:|||..:.|.|.|:.|.:..|..|
  Rat   798 GQGALALAQAVQRASQAPSSFQLLYDLKLSVEDKIRIIAQKIYGADDIELLPEAQNKAEIYTKQG 862

  Fly   890 FGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGLPTRPAIYD 954
            ||||||||:|...|.:.:.:.||.|.||.|.:.|:..|.||||:..:.|.::.||||||||..||
  Rat   863 FGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLPTRPCFYD 927

  Fly   955 IDLNTETGEIEGLF 968
            |||:.||.::.|||
  Rat   928 IDLDPETEQVNGLF 941

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	171/284 (60%)
		THF_DHG_CYH	40..159	CDD:279147	58/112 (52%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	112/170 (66%)
		FTHFS	350..968	CDD:279592	391/617 (63%)
Mthfd1	XP_006240311.1	FolD	17..303	CDD:223268	171/284 (60%)
		THF_DHG_CYH	18..131	CDD:279147	58/112 (52%)
		NAD_bind_m-THF_DH_Cyclohyd	126..298	CDD:133448	113/171 (66%)
		PLN02759	315..941	CDD:178359	394/625 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	785	1.000	Domainoid score	I75
eggNOG	1	0.900	-	-		E1_COG2759
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H55940
Inparanoid	1	1.050	1128	1.000	Inparanoid score	I210
OMA	1	1.010	-	-		QHG53867
OrthoDB	1	1.010	-	-		D690393at2759
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	1	1.000	-	-		otm45661
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	1	1.100	-	-	LDO	PTHR48099
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X878
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.840