DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pug and mthfd1

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_001008007.1	Gene:	mthfd1 / 493369	XenbaseID:	XB-GENE-992132	Length:	934	Species:	Xenopus tropicalis

Alignment Length:	932	Identity:	577/932 - (61%)
Similarity:	721/932 - (77%)	Gaps:	1/932 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 AKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAA 102
            |:|:||..|:..:||.|:.||.||.:::..|.|||.|||||.|:|||:||.||:|||.||||.|:
Frog     3 AEILSGKVVSGLVRERLKAEVAAMKEKVPGFSPGLAIVQVGDRDDSNLYISMKLKAAAEIGISAS 67

  Fly   103 HVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTVNEG 167
            ||:||::.||.|:|.|||.:||||:.||:|||:|||.:.||::.::|:||||.||||||..:|.|
Frog    68 HVKLPKTATEAEVLSKINSINEDPKYHGMIVQLPLDSNHPINTEKVTNAVSPAKDVDGLTCINAG 132

  Fly   168 RLAIGDLGG-FLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHSK 231
            :||.||||. |:||||.||::||:::||:|.|.||||:|||||||.|..:||.|.:||||.||||
Frog   133 KLARGDLGDCFIPCTPKGCMDLIKQTGVQIPGKRAVVIGRSKIVGAPMHDLLLWNHATVTTCHSK 197

  Fly   232 TRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQ 296
            |.:|.|....|||||||.|..|||||.||||||:|||||||...|.:|.||.::||||.|.||.:
Frog   198 TASLNEEVSRADILVVGAGNPEMVKGDWIKPGAIVIDCGINYISDPNKPSGKRVVGDVAYEEAKE 262

  Fly   297 VAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQKP 361
            .|.::||||||||||||||||:|||.||.|:|.:....:|..:...|..:.||||||.|:|:..|
Frog   263 KASYITPVPGGVGPMTVAMLMENTVESAKRYLAQFQPHKWNTEYNKLHLKVPVPSDIEISRSCIP 327

  Fly   362 KDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMGL 426
            |.||.:::||||.:.||.|||..|||:.||.|:||:::|.|.||||.|:|||||||||:||.:||
Frog   328 KPIAKISQEIGLLSEEVELYGQTKAKVLLSTLKRLQNREDGKYVVVTGITPTPLGEGKSTTTIGL 392

  Fly   427 VQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLAA 491
            |||||||...|..|.:||||||||||||||||||||:||||||||||||||||||::|||||:||
Frog   393 VQALGAHLNLNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNLVAA 457

  Fly   492 QLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLDI 556
            .:|.|||||.||.|||||:||||:..|.||||.||:|||::|||.||||..||.:|...|.||||
Frog   458 AIDARIFHELTQSDKALYNRLVPSNNGVRKFSDIQIRRLRRLGIEKTDPTALTEEEINKFVRLDI 522

  Fly   557 DPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLAD 621
            |||||.|:||:|.|||:||.||:||:|||||.:|.|:|.|||||||||||||:..|::|::||..
Frog   523 DPDTITWQRVLDTNDRFLRKITIGQAPTEKGFTRTTQFDISVASEIMAVLALTDGLDNMRERLGK 587

  Fly   622 MVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIADE 686
            ||||..|:|:||:.:||||:||||||:||||:|||||:|||.||.||||||||||||.:||:||:
Frog   588 MVVASSKKGEPVSTEDLGVSGALAVLMKDALKPNLMQTLEGNPVFVHAGPFANIAHGNSSILADK 652

  Fly   687 VGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGAP 751
            :.|||||..|||.||||||:|||||||.:||||.||.:|:.:||||||||:||||||..||.|.|
Frog   653 IALKLVGPEGFVVTEAGFGADIGMEKFFDIKCRYSGLRPHVVVLVATVRALKMHGGGPTVTAGVP 717

  Fly   752 LNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAV 816
            |.|:||||||:||:||..||.:.|||...||:||||::||...||.||.|||...|.:||||.||
Frog   718 LPKEYTEENLDLVEKGCSNLRKQIENATTFGVPVVVAVNAFKTDTEAELELVCHIAKKAGAFDAV 782

  Fly   817 VSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEK 881
            ..||||:||.|||.||.||.:|.|..:.|:.|||:|||:.||:..||..:|||..:.|.|.|::|
Frog   783 RCTHWAEGGKGAVALAQAVQRASEAPSDFKFLYDVELPVADKIRIIAQKIYGADNIELLPEAQKK 847

  Fly   882 VKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPGL 946
            .:..|..||||||:||:|...|.:.:.::||.|.||.|.:.|:..|.||||:..:.|.::.||||
Frog   848 AELYTKQGFGNLPVCMAKTHLSLSHNPELKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGL 912

  Fly   947 PTRPAIYDIDLNTETGEIEGLF 968
            ||||..|||||:..:.::.|||
Frog   913 PTRPCFYDIDLDPVSEQVNGLF 934

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	180/291 (62%)
		THF_DHG_CYH	40..159	CDD:279147	68/118 (58%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	111/170 (65%)
		FTHFS	350..968	CDD:279592	390/617 (63%)
mthfd1	NP_001008007.1	FolD	5..295	CDD:223268	179/289 (62%)
		THF_DHG_CYH	5..124	CDD:279147	68/118 (58%)
		NAD_bind_m-THF_DH_Cyclohyd	119..291	CDD:133448	112/171 (65%)
		PLN02759	308..934	CDD:178359	393/625 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	797	1.000	Domainoid score	I78
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H55940
Inparanoid	1	1.050	1146	1.000	Inparanoid score	I214
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D690393at2759
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	1	1.000	-	-		oto104153
Panther	1	1.100	-	-	LDO	PTHR48099
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X878
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	8	8.160