DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment pug and MTHFD1

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_001351766.1	Gene:	MTHFD1 / 4522	HGNCID:	7432	Length:	964	Species:	Homo sapiens

Alignment Length:	908	Identity:	556/908 - (61%)
Similarity:	692/908 - (76%)	Gaps:	1/908 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    35 MSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGI 99
            |:.|:|::|..::..||..|:|:||.:.:|:..|.|.|.|:|||.|:|||:||.:|:|||.||||
Human     1 MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGI 65

  Fly   100 DAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTV 164
            .|.|::|||:.||.|::..|..||||..|||.:||:|||.:..|::..:.:|::||||||||.::
Human    66 KATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSI 130

  Fly   165 NEGRLAIGDLGG-FLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVC 228
            |.|:||.|||.. |:||||.||||||:.:||.|||..|||:|||||||.|..:||.|.|||||.|
Human   131 NAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTC 195

  Fly   229 HSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAE 293
            ||||.:|:|.....|||||..|..|||||.||||||:|||||||..||..|.:|.|:||||.|.|
Human   196 HSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDE 260

  Fly   294 ALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARA 358
            |.:.|..:||||||||||||||||::||.||.||||:....:|.:|...|..:.||||||.|:|:
Human   261 AKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMIQYNNLNLKTPVPSDIDISRS 325

  Fly   359 QKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTL 423
            .|||.|..||:||||.:.||.|||..|||:.||.|||||.:..|.||||.|:|||||||||:||.
Human   326 CKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTT 390

  Fly   424 MGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNL 488
            :|||||||||..:|..|.:||||||||||||||||||||:||||||||||||||||||::|||||
Human   391 IGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNL 455

  Fly   489 LAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFAR 553
            :||.:|.|||||.||.||||::||||::.|.|:||.||:|||::|||.||||.|||.:|...|||
Human   456 VAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRLGIEKTDPTTLTDEEINRFAR 520

  Fly   554 LDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQR 618
            |||||:||.|:||:|.|||:||.||:||:|||||.:|..:|.|||||||||||||:.|||||::|
Human   521 LDIDPETITWQRVLDTNDRFLRKITIGQAPTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRER 585

  Fly   619 LADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSII 683
            |..||||..|:|:||:|:||||:|||.||:|||::|||||:||||||.||||||||||||.:|||
Human   586 LGKMVVASSKKGEPVSAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSII 650

  Fly   684 ADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTP 748
            ||.:.|||||..|||.||||||:|||||||.|||||.||..|:.:||||||||:||||||..||.
Human   651 ADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTA 715

  Fly   749 GAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAF 813
            |.||.|.|.:||||||:||..||.:.|||.:.||:||||::||...||.:|.:|:.:.:.|.|||
Human   716 GLPLPKAYIQENLELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAF 780

  Fly   814 AAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAA 878
            .||..||||:||.||:.||.||.:|.:..:.|:|||||:||:.||:..||..:|||..:.|.|.|
Human   781 DAVKCTHWAEGGKGALALAQAVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEA 845

  Fly   879 EEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVT 941
            :.|.:..|..|||||||||:|...|.:.:.:.||.|.||.|.:.|:..|.||||:..:.|.:|
Human   846 QHKAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTIT 908

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	170/291 (58%)
		THF_DHG_CYH	40..159	CDD:279147	57/118 (48%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	112/170 (66%)
		FTHFS	350..968	CDD:279592	378/592 (64%)
MTHFD1	NP_001351766.1	Methylenetetrahydrofolate dehydrogenase and cyclohydrolase	2..305		173/302 (57%)
		FolD	6..294	CDD:223268	167/287 (58%)
		Substrate binding	52..56		2/3 (67%)
		Substrate binding	99..101		1/1 (100%)
		Substrate binding	272..276		3/3 (100%)
		PLN02759	310..907	CDD:178359	380/596 (64%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	785	1.000	Domainoid score	I76
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H55940
Inparanoid	1	1.050	1139	1.000	Inparanoid score	I217
Isobase	1	0.950	-	0.875	Normalized mean entropy	S317
OMA	1	1.010	-	-		QHG53867
OrthoDB	1	1.010	-	-		D690393at2759
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	1	1.000	-	-		oto90347
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	1	1.100	-	-	LDO	PTHR48099
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R159
SonicParanoid	1	1.000	-	-		X878
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	14	13.960