DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pug and Mthfd1l

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_001101932.2	Gene:	Mthfd1l / 361472	RGDID:	1307311	Length:	977	Species:	Rattus norvegicus

Alignment Length:	933	Identity:	486/933 - (52%)
Similarity:	632/933 - (67%)	Gaps:	43/933 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly    50 IREELRNE---VTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSIT 111
            :|:.:.|.   :..:.::...|.|.|.::|.|   |.::...:....|.|.|:|..|:.||....
  Rat    74 VRDVIHNSKEVLNLLQEKNPAFKPVLAVIQAG---DDHLMQNVNQNLAKEAGLDITHICLPPDSG 135

  Fly   112 EVELLDKINDLNEDPRVHGIIVQMPLD--CDTPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDL 174
            |.|::|:|..:||||||||:.:|:..|  |      :::.:|:.|||||||:..||.|:|..||.
  Rat   136 EDEIIDEILKINEDPRVHGLALQISEDSLC------NKVLNALKPEKDVDGVTDVNLGKLVRGDA 194

  Fly   175 GG-FLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCHS-KTRNLEE 237
            .. |:.......:|||.:||:.:.|.:.:|:|....: ..|.:.|.....::|:... .|..|::
  Rat   195 PECFVSPLARAAVELIEKSGITLDGKKVLVIGAHGPL-EAALQCLFQRKGSMTMSSPWITPQLQD 258

  Fly   238 ITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASG-------SKLVGDVDYAEAL 295
            ..|.|||:|:|....:.:..:||..||.:::|..:..  :.|..|       ::|:.|
  Rat   259 KLREADIVVLGSPKPDEIPAAWIPSGATMLNCFHDFL--SGKLRGGSPGVPMNRLIAD------- 314

  Fly   296 QVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARAQK 360
                      ..|..:..|:.::|.|.|..|:|......:|.|..|.|:|..||||||.|:|.|.
  Rat   315 ----------ESVSLLAAALRVQNMVNSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQT 369

  Fly   361 PKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTLMG 425
            ||.:.||||||||.|.|:.:||..|||:.||:||||||:..|.||:|||:|||||||||:|..:|
  Rat   370 PKAVDVLAKEIGLLADEIEIYGKSKAKVRLSLLERLKDQTDGKYVLVAGITPTPLGEGKSTVTIG 434

  Fly   426 LVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNLLA 490
            |||||.||...|:.|.|||||||||||:||||||||||||||||||||||||||||::|||||||
  Rat   435 LVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLA 499

  Fly   491 AQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFARLD 555
            |.:|||||||:||.|||||:||||.:.|.||||.|||.||:||||.||||..||.:|...|.||:
  Rat   500 AAIDTRIFHESTQTDKALYNRLVPLVNGVRKFSEIQLSRLKKLGIHKTDPTALTEEEVRKFTRLN 564

  Fly   556 IDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQRLA 620
            |||.||.|:||:|.|||:||.||:||..||||.||:.:|.|:|||||||||||:.||.|||:||.
  Rat   565 IDPSTITWQRVLDTNDRFLRKITIGQGSTEKGYSRQAQFDIAVASEIMAVLALTDSLADMKERLG 629

  Fly   621 DMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSIIAD 685
            .||||.||.|:||||:||||||||.||:|||::|||||:||||||.||||||||||||.:|::||
  Rat   630 RMVVASDKDGQPVTAEDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLAD 694

  Fly   686 EVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTPGA 750
            ::.|||||:.|||.||||||:|||||||.|||||.||..||.:||||||||:||||||..||.|.
  Rat   695 KIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGV 759

  Fly   751 PLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAA 815
            ||.|:|||||::||..|..||.:.|:..:.||:||||:||....||.||.:||.:.|..||||.|
  Rat   760 PLKKEYTEENIQLVADGCCNLQKQIQIAQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDA 824

  Fly   816 VVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEE 880
            |...||:.||.|:|.||.||.:|..:.::||.|||::||:|.|:..||.|:|||..:.|||.|:.
  Rat   825 VPCYHWSAGGKGSVDLARAVREAANKRSRFRFLYDVQLPIVAKIRVIAQTVYGAKDIELSPEAQA 889

  Fly   881 KVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKMPG 945
            |:.|.|..|||||||||:|...|.:.:...||.|:.|.|.:.||..|.||||:..:.|.::.|||
  Rat   890 KIDRYTQQGFGNLPICMAKTHLSLSHEPDKKGVPRDFILPISDVRASIGAGFIYPLVGMMSTMPG 954

  Fly   946 LPTRPAIYDIDLNTETGEIEGLF 968
            |||||..|||||:|||.:::|||
  Rat   955 LPTRPCFYDIDLDTETEQVKGLF 977

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	80/293 (27%)
		THF_DHG_CYH	40..159	CDD:279147	35/113 (31%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	46/178 (26%)
		FTHFS	350..968	CDD:279592	397/617 (64%)
Mthfd1l	NP_001101932.2	THF_DHG_CYH	73..179	CDD:279147	35/113 (31%)
		FolD	82..>285	CDD:223268	64/212 (30%)
		NADB_Rossmann	192..334	CDD:304358	36/161 (22%)
		PLN02759	351..977	CDD:178359	401/625 (64%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	785	1.000	Domainoid score	I75
eggNOG	1	0.900	-	-		E1_COG2759
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53867
OrthoDB	1	1.010	-	-		D690393at2759
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	1	1.100	-	-	O	PTHR48099
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X878
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	11	10.790