DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment pug and Mthfd2l

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_001100681.1	Gene:	Mthfd2l / 305248	RGDID:	1310879	Length:	338	Species:	Rattus norvegicus

Alignment Length:	324	Identity:	133/324 - (41%)
Similarity:	186/324 - (57%)	Gaps:	16/324 - (4%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 LEKWPAEKSKVGSGEWTA-EPAIKMSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIV 75
            |.:.||....|....|.. ..:.:...|.|||||.:||.||.||:..|.:. ..|.:..|.|.|:
  Rat    15 LGRGPARAPGVAERAWRGFGSSSRRHEAVIISGTEMAKQIRRELQQGVESW-LALGNRRPHLSII 78

  Fly    76 QVGGREDSNVYIRMKIKAATEIGIDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCD 140
            .||....|:.|:|.||:||:.:||.:..:..|:::::.||||..:.||.||||.||:||:||  .
  Rat    79 LVGDNPASHTYVRNKIRAASAVGICSELIVKPKNVSQEELLDITDQLNMDPRVSGILVQLPL--P 141

  Fly   141 TPIDSHRITDAVSPEKDVDGLHTVNEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLG 205
            ..:|...|.:.::|||||||.|.:|.|||.: |....:|.|.....|:|:|:|:|..|...||.|
  Rat   142 DHVDERTICNGIAPEKDVDGFHIINIGRLCL-DQHSLIPATASAVWEIIKRAGIETFGKNVVVAG 205

  Fly   206 RSKIVGTPAAELL--------KWANATVTVCHSKT--RNLEEITRSADILVVGIGVAEMVKGSWI 260
            |||.||.|.|.||        ...:||||:.|..|  ..|:..|:.|:|::|..|:..::....:
  Rat   206 RSKNVGMPIAMLLHTDGEHERPGGDATVTIAHRHTPREQLKAHTQLAEIIIVAAGIPGLITADMV 270

  Fly   261 KPGAVVIDCGINVKPDASKASGSKLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSA 324
            :.||.|||.|||...|..... :|||||||:....:.|..:|||||||||||||||:|||:.:|
  Rat   271 REGATVIDVGINYVQDPVTGK-TKLVGDVDFEAVKKKASFITPVPGGVGPMTVAMLLKNTLLAA 333

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	127/296 (43%)
		THF_DHG_CYH	40..159	CDD:279147	50/118 (42%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	80/179 (45%)
		FTHFS	350..968	CDD:279592
Mthfd2l	NP_001100681.1	FolD	44..335	CDD:223268	127/295 (43%)
		THF_DHG_CYH	44..160	CDD:279147	50/118 (42%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P13995	89..93		2/3 (67%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P13995	136..138		1/1 (100%)
		NAD_bind_m-THF_DH_Cyclohyd	155..334	CDD:133448	81/181 (45%)
		Substrate binding. /evidence=ECO:0000250\|UniProtKB:P13995	314..318		3/3 (100%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810