DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pug and AgaP_AGAP002830

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	XP_312083.5	Gene:	AgaP_AGAP002830 / 1273131	VectorBaseID:	AGAP002830	Length:	937	Species:	Anopheles gambiae

Alignment Length:	936	Identity:	657/936 - (70%)
Similarity:	779/936 - (83%)	Gaps:	2/936 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    35 MSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGI 99
            ::||||:|||.|||.|||.|:.||..:.:|:...||||.|||||||||||||||||||||.|||:
Mosquito     2 LNGAKILSGTDVAKEIRERLKKEVAEIRRQVPTLVPGLAIVQVGGREDSNVYIRMKIKAAEEIGM 66

  Fly   100 DAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTV 164
            .|.|||||||.|:.|||..:..|||||..||||||||||.:||||:|.||||||||||||||:|:
Mosquito    67 YAEHVQLPRSTTQDELLATVKRLNEDPCFHGIIVQMPLDSETPIDAHLITDAVSPEKDVDGLNTI 131

  Fly   165 NEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVCH 229
            ||||:|.||:.|||||||.||:|||:|:|..|||:.|||:|||||||||.:||||||:|||||.|
Mosquito   132 NEGRVATGDMSGFLPCTPNGCMELIKRTGTPIAGSYAVVIGRSKIVGTPMSELLKWADATVTVAH 196

  Fly   230 SKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAEA 294
            |||:||..:.:.||||||.:|..|||:|:||||||||||||||..||.:|.||.:|||||.|.||
Mosquito   197 SKTKNLNLLIKKADILVVAVGRPEMVRGAWIKPGAVVIDCGINSIPDPTKKSGQRLVGDVKYEEA 261

  Fly   295 LQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQ--WALQTLPLKPQRPVPSDIVIAR 357
            |.||.|:||||||||||||||||:|||:||.|...||.:.:  |.|:.||||...||||||.|||
Mosquito   262 LPVASHVTPVPGGVGPMTVAMLMQNTVQSAQRVARRLIEMEFRWCLKPLPLKLTEPVPSDIEIAR 326

  Fly   358 AQKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTT 422
            ||:|||:||||:||||...||||||.||||:||.||:||:.::...||||||:|||||||||:||
Mosquito   327 AQEPKDVAVLAQEIGLIPSEVSLYGTKKAKVSLKVLQRLQYEDDAKYVVVAGITPTPLGEGKSTT 391

  Fly   423 LMGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANN 487
            |:||||||.|||..|::|.|||||||||||||||||||||:||||||:||||||||||||:||||
Mosquito   392 LVGLVQALTAHKRCNSIACLRQPSQGPTFGIKGGAAGGGYSQVIPMEDFNLHLTGDIHAVTAANN 456

  Fly   488 LLAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFA 552
            |||||||.|||||.||.|:|||:|||||:||.|||:|||||||::|||.||||..||.||...||
Mosquito   457 LLAAQLDARIFHEATQTDQALYERLVPAVKGSRKFAPIQLRRLKRLGIEKTDPYALTPDEIRRFA 521

  Fly   553 RLDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQ 617
            ||:|||..::|.||:|:||||||.||:|.|||||.::|:|.|:|||:|||||:|||:..||||||
Mosquito   522 RLNIDPHNVVWTRVLDVNDRYLRKITIGLSPTEKNLTRDTSFAISVSSEIMAILALATGLEDMKQ 586

  Fly   618 RLADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSI 682
            |||.|||||||.|.|||||||||||::.||||||:||.|||:||||||||||||||||||||:||
Mosquito   587 RLAKMVVAFDKAGTPVTADDLGVTGSMMVLLKDAIEPTLMQTLEGTPVLVHAGPFANIAHGCSSI 651

  Fly   683 IADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVT 747
            :||::|:||.||.|:|.||||||||||||||.|||||.||:.|||:|||.||||:||||||..||
Mosquito   652 VADDIGMKLAGKRGYVVTEAGFGSDIGMEKFFNIKCRASGKVPNAVVLVTTVRALKMHGGGPIVT 716

  Fly   748 PGAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGA 812
            .||||.::|..|||:|::|||||||:|||||..:|:||||::|||..||.||||||:|||..|||
Mosquito   717 AGAPLRREYMSENLDLLEKGLPNLLKHIENGLKYGVPVVVAINAHGTDTAAEHELVQKAAKAAGA 781

  Fly   813 FAAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPA 877
            |.|:||.|||.||.||.:||.|||.||.:.::|:|||||::.:.||:.:||..|||||.:.|:..
Mosquito   782 FDAIVSNHWAKGGEGAAELAQAVIDACNRPSEFKLLYDLDMSIEDKIVRIAKEMYGAGTIELNSK 846

  Fly   878 AEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTK 942
            ..:.::..|:.|||.|||||:|.|.|.|||...|||||.|.|.:.|:.:||||||:|.||||::|
Mosquito   847 VRDAIRLYTEKGFGKLPICMAKTSMSLTGDPSAKGAPKDFKLVINDICLSAGAGFIVPMCGEISK 911

  Fly   943 MPGLPTRPAIYDIDLNTETGEIEGLF 968
            ||||||||:|:|||||||||||||||
Mosquito   912 MPGLPTRPSIFDIDLNTETGEIEGLF 937

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	207/290 (71%)
		THF_DHG_CYH	40..159	CDD:279147	84/118 (71%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	123/169 (73%)
		FTHFS	350..968	CDD:279592	436/617 (71%)
AgaP_AGAP002830	XP_312083.5	FolD	6..297	CDD:223268	207/290 (71%)
		THF_DHG_CYH	7..126	CDD:279147	84/118 (71%)
		NAD_bind_m-THF_DH_Cyclohyd	121..292	CDD:133448	124/170 (73%)
		FTHFS	319..937	CDD:279592	436/617 (71%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	889	1.000	Domainoid score	I197
eggNOG	1	0.900	-	-		E1_COG2759
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H55940
Inparanoid	1	1.050	1319	1.000	Inparanoid score	I490
OMA	1	1.010	-	-		QHG53867
OrthoDB	1	1.010	-	-		D41938at7147
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	1	1.000	-	-		oto109121
Panther	1	1.100	-	-	LDO	PTHR48099
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X878
TreeFam	1	0.960	-	-
	13	12.940