DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pug and Mthfd1

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_620084.2	Gene:	Mthfd1 / 108156	MGIID:	1342005	Length:	935	Species:	Mus musculus

Alignment Length:	935	Identity:	577/935 - (61%)
Similarity:	714/935 - (76%)	Gaps:	1/935 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    35 MSGAKIISGTAVAKSIREELRNEVTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIGI 99
            |:.|.|::|..|:..||:.|:|:||.|.:|:..|.|||.|:|||.|:|||:||.:|:|||.||||
Mouse     1 MAPAGILNGKLVSAQIRDRLKNQVTRMQEQVPGFTPGLAILQVGDRDDSNLYINVKLKAAEEIGI 65

  Fly   100 DAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHTV 164
            .|.|::|||:.||.|:|..:..||||..|||.|||:|||.:..|::..:.:|::||||||||.:|
Mouse    66 KATHIKLPRTSTESEVLKYVISLNEDASVHGFIVQLPLDSENSINTEAVINAIAPEKDVDGLTSV 130

  Fly   165 NEGRLAIGDLGG-FLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELLKWANATVTVC 228
            :.|:||.|||.. |:||||.||||||:.:||:|||..|||:|||||||.|..:||.|.|||||.|
Mouse   131 SAGKLARGDLNDCFIPCTPKGCLELIKEAGVQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTC 195

  Fly   229 HSKTRNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGSKLVGDVDYAE 293
            ||||.||::.....|||||..|..|||||.||||||||||||||..||.:|.:|.|:||||.|.|
Mouse   196 HSKTANLDKEVNKGDILVVATGQPEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDE 260

  Fly   294 ALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFLERLAKSQWALQTLPLKPQRPVPSDIVIARA 358
            |.:.|..:||||||||||||||||::||.||.|||::....:|.:|...|..:.||||||.|:|:
Mouse   261 AKERASFITPVPGGVGPMTVAMLMQSTVESAQRFLQKFKPGKWTIQYNKLNLKTPVPSDIAISRS 325

  Fly   359 QKPKDIAVLAKEIGLEAREVSLYGNKKAKISLSVLERLKDKEVGHYVVVAGMTPTPLGEGKTTTL 423
            .|||.|..||:||||...||.|||..|||:.||.|:|||.:..|.||||.|:|||||||||:||.
Mouse   326 CKPKLIGNLAREIGLLTEEVELYGETKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTT 390

  Fly   424 MGLVQALGAHKLRNTMAALRQPSQGPTFGIKGGAAGGGYAQVIPMEEFNLHLTGDIHAVSAANNL 488
            :|||||||||..:|..|.:||||||||||||||||||||:||||||||||||||||||::|||||
Mouse   391 IGLVQALGAHLRQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAITAANNL 455

  Fly   489 LAAQLDTRIFHENTQKDKALYDRLVPAIKGQRKFSPIQLRRLQKLGITKTDPDTLTADEYGPFAR 553
            :||.:|.|||||.||.||||::||||::.|.||||.||:|||::|||.||||.|||.||...|||
Mouse   456 VAAAIDARIFHELTQTDKALFNRLVPSVNGIRKFSDIQIRRLRRLGIEKTDPTTLTDDEINRFAR 520

  Fly   554 LDIDPDTIMWERVVDINDRYLRTITVGQSPTEKGISRETRFSISVASEIMAVLALSRSLEDMKQR 618
            |||||:||.|:||:|.|||:||.||:||||||||.:|..:|.|||||||||||||:.|||||::|
Mouse   521 LDIDPETITWQRVLDTNDRFLRKITIGQSPTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRER 585

  Fly   619 LADMVVAFDKRGKPVTADDLGVTGALAVLLKDALEPNLMQSLEGTPVLVHAGPFANIAHGCNSII 683
            |..||||..|:|:|::.:||||:|||.||:|||::|||||:||||||.||||||||||||.:|||
Mouse   586 LGRMVVASSKKGEPISCEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSII 650

  Fly   684 ADEVGLKLVGKNGFVCTEAGFGSDIGMEKFCNIKCRTSGRKPNAMVLVATVRAIKMHGGGAPVTP 748
            ||.:.|||||..|||.||||||:|||||||.|||||.||.:|:.:||||||||:||||||..||.
Mouse   651 ADRIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLQPHVVVLVATVRALKMHGGGPTVTA 715

  Fly   749 GAPLNKQYTEENLELVQKGLPNLLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAF 813
            |.||.|.||||:|:||:||..||.:.|||.:.||:||||::|....||.||.:||.:.:.|.|||
Mouse   716 GLPLPKAYTEEDLDLVEKGFSNLRKQIENARMFGVPVVVAVNVFKTDTDAELDLVSRLSREHGAF 780

  Fly   814 AAVVSTHWADGGAGAVQLADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAA 878
            .||..||||:||.||:.||.||.:|.:..:.|:|||||:|.:.||:..||..:|||..:.|.|.|
Mouse   781 DAVKCTHWAEGGQGALALAQAVQRASQAPSSFQLLYDLKLSIEDKIRIIAQRIYGADDIELLPEA 845

  Fly   879 EEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSAGAGFVVAMCGEVTKM 943
            :.|.:..|..|||||||||:|...|.:.:.:.||.|.||.|.:.|:..|.||||:..:.|.::.|
Mouse   846 QNKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTM 910

  Fly   944 PGLPTRPAIYDIDLNTETGEIEGLF 968
            |||||||..|||||:.||.::.|||
Mouse   911 PGLPTRPCFYDIDLDPETEQVNGLF 935

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	175/291 (60%)
		THF_DHG_CYH	40..159	CDD:279147	61/118 (52%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	113/170 (66%)
		FTHFS	350..968	CDD:279592	393/617 (64%)
Mthfd1	NP_620084.2	Methylenetetrahydrofolate dehydrogenase and cyclohydrolase	1..305		178/303 (59%)
		FolD	6..294	CDD:223268	172/287 (60%)
		THF_DHG_CYH	6..125	CDD:279147	61/118 (52%)
		Substrate binding. /evidence=ECO:0000250	52..56		2/3 (67%)
		Substrate binding. /evidence=ECO:0000250	99..101		1/1 (100%)
		NAD_bind_m-THF_DH_Cyclohyd	120..292	CDD:133448	114/171 (67%)
		Substrate binding. /evidence=ECO:0000250	272..276		3/3 (100%)
		Formyltetrahydrofolate synthetase	306..935		397/628 (63%)
		PLN02759	309..935	CDD:178359	396/625 (63%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	786	1.000	Domainoid score	I74
eggNOG	1	0.900	-	-		E1_COG2759
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H55940
Inparanoid	1	1.050	1139	1.000	Inparanoid score	I215
Isobase	1	0.950	-	0.875	Normalized mean entropy	S317
OMA	1	1.010	-	-		QHG53867
OrthoDB	1	1.010	-	-		D690393at2759
OrthoFinder	1	1.000	-	-		FOG0001266
OrthoInspector	1	1.000	-	-		oto93930
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	1	1.100	-	-	LDO	PTHR48099
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R159
SonicParanoid	1	1.000	-	-		X878
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.820