DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment pug and MTHFD2

DIOPT Version :9

Sequence 1:	NP_001014614.1	Gene:	pug / 41279	FlyBaseID:	FBgn0020385	Length:	968	Species:	Drosophila melanogaster
Sequence 2:	NP_006627.2	Gene:	MTHFD2 / 10797	HGNCID:	7434	Length:	350	Species:	Homo sapiens

Alignment Length:	305	Identity:	136/305 - (44%)
Similarity:	183/305 - (60%)	Gaps:	23/305 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly    38 AKIISGTAVAKSIREELRNE----VTAMSKQLADFVPGLRIVQVGGREDSNVYIRMKIKAATEIG 98
            |.:|||..:|:.|::|:|.|    |.:.:|:     |.|.::.||....|:.|:..|.:||..:|
Human    37 AVVISGRKLAQQIKQEVRQEVEEWVASGNKR-----PHLSVILVGENPASHSYVLNKTRAAAVVG 96

  Fly    99 IDAAHVQLPRSITEVELLDKINDLNEDPRVHGIIVQMPLDCDTPIDSHRITDAVSPEKDVDGLHT 163
            |::..:..|.||:|.|||:.||.||.|..|.|::||:||  ...||..||.:||||:|||||.|.
Human    97 INSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPL--PEHIDERRICNAVSPDKDVDGFHV 159

  Fly   164 VNEGRLAIGDLGGFLPCTPWGCLELIRRSGVEIAGARAVVLGRSKIVGTPAAELL--------KW 220
            :|.||:.: |....||.||||..|:|:|:|:...|...||.||||.||.|.|.||        ..
Human   160 INVGRMCL-DQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPG 223

  Fly   221 ANATVTVCHSKT--RNLEEITRSADILVVGIGVAEMVKGSWIKPGAVVIDCGINVKPDASKASGS 283
            .:||||:.|..|  ..|::.|..|||::...|:..::....||.||.|||.|||...|...|. .
Human   224 GDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAK-P 287

  Fly   284 KLVGDVDYAEALQVAGHLTPVPGGVGPMTVAMLMKNTVRSAARFL 328
            |||||||:....|.||::|||||||||||||||||||:.:|.:.|
Human   288 KLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVL 332

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pug	NP_001014614.1	FolD	39..330	CDD:223268	135/304 (44%)
		THF_DHG_CYH	40..159	CDD:279147	49/122 (40%)
		NAD_bind_m-THF_DH_Cyclohyd	154..324	CDD:133448	87/179 (49%)
		FTHFS	350..968	CDD:279592
MTHFD2	NP_006627.2	FolD	39..334	CDD:223268	135/303 (45%)
		THF_DHG_CYH	39..155	CDD:279147	49/122 (40%)
		Substrate binding. /evidence=ECO:0000269\|PubMed:27899380	84..88		1/3 (33%)
		Substrate binding. /evidence=ECO:0000269\|PubMed:27899380	131..133		1/1 (100%)
		NAD_bind_m-THF_DH_Cyclohyd	150..329	CDD:133448	87/180 (48%)
		Substrate binding. /evidence=ECO:0000269\|PubMed:27899380	309..313		3/3 (100%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165156861
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100216
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.740