DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rfx and rfx3

DIOPT Version :9

Sequence 1:	NP_001247023.2	Gene:	Rfx / 41266	FlyBaseID:	FBgn0020379	Length:	919	Species:	Drosophila melanogaster
Sequence 2:	NP_001073517.1	Gene:	rfx3 / 570989	ZFINID:	ZDB-GENE-061103-253	Length:	765	Species:	Danio rerio

Alignment Length:	789	Identity:	342/789 - (43%)
Similarity:	427/789 - (54%)	Gaps:	157/789 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly   154 TTEPNGTTVTHSIPIHSMADLAAIKDGVDLAQQVANGQVTVVQTTEDDDGTPFITVTVSGQEQNY 218
            |.|....:...::|:.:...:.....|    ||||: ||.|.|..:          ||...:..|
Zfish     3 TPEAGADSTVSTVPLQTSVPVQPAVSG----QQVAS-QVPVQQQAQ----------TVQQVQHVY 52

  Fly   219 --QVQYVDSELYHSNSSQTQMT---YPFCPVGDYQGNGQTAYYSTTGQYG---------TTSSAG 269
              |||||..|   :||..|..|   |.:.....|..|....|:.|.|..|         :|.|..
Zfish    53 QAQVQYVQEE---NNSVYTNGTIRAYSYSEPQLYNQNSSGNYFDTQGGGGSAAQVSTVVSTHSMA 114

  Fly   270 GSSNG----AHSTTLPYLVPVEEGILLNGSAHSLSQSQSQSHGRDSPHSLTVSSRDWERDADRVN 330
            .:.||    |...|...::......|:.|:    |...|..||.
Zfish   115 NNGNGTGALAMGLTGGQIISSSGAYLIGGN----SMDGSAPHGA--------------------- 154

  Fly   331 GTNRHWVQEVAYIQEAQSTPQTPTSTTTTHSASGGSLGTGGGGASPDSDQSALGSSNKIASATIK 395
                        .|..:::|.|......|...|.|.          .|.:|:|.:|:      ::
Zfish   155 ------------AQTTRASPATIEMAIETLQKSEGL----------SSQRSSLLNSH------LQ 191

  Fly   396 WLSRNYETADGVSLPRSTLYNHYMQHCSEHKLEPVNAASFGKLIRSVFSGLRTRRLGTRGNSKYH 460
            ||..|||||:|||||||||||||::||.|.||:|||||||||||||:|.||||||||||||||||
Zfish   192 WLLDNYETAEGVSLPRSTLYNHYLRHCQEQKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYH 256

  Fly   461 YYGIRIKPGSLLNSQAMDDKQMLAAGYGP-----------SSDGTGGPGSGPMVSVTSSTAGQLT 514
            |||||:||.|.|| :..:|.|.:|....|           ..||..|.        ..|:|||
Zfish   257 YYGIRVKPDSPLN-RLQEDMQYMALRQQPVQQKQRFKPVQKMDGVSGD--------NFSSAGQ-- 310

  Fly   515 GSNGLGGGHGQRHSNGTKKHTFKPETYEACIQYIGDGTSALPSFPPIELNHSFNSELTLEDVDTF 579
                        |:....:.||..::...  |...||:.|||.|..::|..:.:. :..|||...
Zfish   311 ------------HTPSAAEQTFIAQSQHH--QQFLDGSRALPEFVELDLGEAVDG-VGPEDVKAL 360

  Fly   580 RGLYREHCESFLDAVLNLEFNTVEFLLRDFWRAS-----------DNNNLDECEEEKYLSKTKLY 633
            :.|||||||:.||.|:||:||.:|.|.:.|||.|           :|:.|.|.|..  |.:.:|.
Zfish   361 QTLYREHCEAILDVVVNLQFNLIENLWQTFWRYSASSSVEGVTITENSGLSEIEGR--LPRARLI 423

  Fly   634 LLCHCAEVQKFVREVDYQFYQNTVDVIIPDVLRSIPNALTQAIRNFAKNLEIWLCESMLGVPEQL 698
            |||....|.|::...|:..||..|:::||||||.||:|||||||||||:||.||..:|..:|.::
Zfish   424 LLCRHEAVHKWMNTCDHLMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLTNAMSSIPPRM 488

  Fly   699 AQIKTSAVSAFCQTLRRYTSLNHLAQAARAVLQNGAQISQMLSDLNRVDFHNVQEQAAWVSQCAP 763
            ...|.||||||.||||||||||||||||||||||.:||:|||||||||||.||||||:||.||..
Zfish   489 INTKVSAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEE 553

  Fly   764 AVVQRLESDFKAALQQQSSLEQWASWLQLVVESAMEEYNGKPTYARAARQFLLKWSFYSSMIIRD 828
            .||||||.||||.|||||||||||:||..||...::.|..|||..:|||||||||||||||:|||
Zfish   554 GVVQRLEQDFKATLQQQSSLEQWAAWLDNVVTQILKPYEDKPTLPKAARQFLLKWSFYSSMVIRD 618

  Fly   829 LTLRSASSFGSFHLIRLLFDEYMFYLVEHKIAEAQDKTAIAVICE------------------RM 875
            ||||||:|||||||||||:||||||||||::|:|..:|||.|:.|                  .|
Zfish   619 LTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETAIGVMGEFQDLNTMSPANIDKDEVSEM 683

  Fly   876 KKDMDFEFE 884
            ..|:|.|.|
Zfish   684 DSDLDEEME 692

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rfx	NP_001247023.2	RFX_DNA_binding	393..466	CDD:280427	59/72 (82%)
rfx3	NP_001073517.1	RFX1_trans_act	<2..141	CDD:282451	38/155 (25%)
rfx3	NP_001073517.1	RFX_DNA_binding	190..262	CDD:280427	59/71 (83%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170596121
Domainoid	1	1.000	389	1.000	Domainoid score	I755
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H7917
Inparanoid	1	1.050	528	1.000	Inparanoid score	I1191
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D278246at33208
OrthoFinder	1	1.000	-	-		FOG0000699
OrthoInspector	1	1.000	-	-		otm25965
orthoMCL	1	0.900	-	-		OOG6_102578
Panther	1	1.100	-	-	LDO	PTHR12619
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1282
SonicParanoid	1	1.000	-	-		X291
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.930