Protein Alignment Rfx and rfx4

DIOPT Version :10

• Sequence 1: NP_001247023.2
• Gene: Rfx / 41266
• FlyBaseID: FBgn0020379
• Length: 919
• Species: Drosophila melanogaster
• Sequence 2: NP_991275.2
• Gene: rfx4 / 403016
• ZFINID: ZDB-GENE-040909-2
• Length: 735
• Species: Danio rerio
• Alignment Length: 604
• Identity: 196/604 - (32%)
• Similarity: 281/604 - (46%)
• Gaps: 105/604 - (17%)

  Fly   323 ERDADRVNGTNRHWVQEVAYIQEAQSTPQTPTSTTTTHSASGGSLGTGGGGASPDSDQSALGSSN 387
            |.|.|    :...|::..  :.|::|      ...::||:.|          :..:|::....:|
Zfish     8 EPDMD----STESWIERC--LNESES------KRFSSHSSIG----------NISNDENEEKENN 50

  Fly   388 KIAS-----ATIKWLSRNYETADGVSLPRSTLYNHYMQHCSEHKLEPVNAASFGKLIRSVFSGLR 447
            :.:.     ||::||..|||.|:||.:|||.||.||:..|.:...:|||||||||:||..|..|.
Zfish    51 RASKPHSTPATLQWLEENYEIAEGVCIPRSALYMHYLDFCEKLDSQPVNAASFGKIIRQQFPQLT 115

  Fly   448 TRRLGTRGNSKYHYYGIRIKPGSLLNSQAMDDKQMLAAGYGPSSDGTGGPGSGPMVSVTSSTAGQ 512
            ||||||||.||||||||.:|..|          |.....|  |..|...........||..|.
Zfish   116 TRRLGTRGQSKYHYYGIAVKESS----------QYYDVMY--SKKGAAWVNETGKKEVTKQTV-- 166

  Fly   513 LTGSNGLGGGHGQRHSNGTKKHTFKPETYEACIQYIGDGTSALPSFPPI-ELNHSFNSELTLEDV 576
                     .:..|...||                      .||.||.: :||  ..:.|..|.|
Zfish   167 ---------AYSPRSKLGT----------------------LLPDFPNVKDLN--LPASLPEEKV 198

  Fly   577 DTFRGLYREHCESFLDAVLNLEFNTVEFLLRDFWRASDNNNLDECEEEKYLSKTKLYLLCHCAEV 641
            .||..:||.||:..||.|:...|:.|:..|..||:....:.|.               :...:.|
Zfish   199 STFIMMYRTHCQRILDTVIRANFDEVQSFLLHFWQGMPPHMLP---------------VLGSSTV 248

  Fly   642 QKFVREVDYQFYQNTVDVIIPDVLRSIPNALTQAIRNFAKNLEIWLCESMLGVPEQLAQIKTSAV 706
            ...|...|...|:....|::|.||:::|::|||.||.|||.|:.||..::..:||.|..||....
Zfish   249 VNIVGVCDSILYKAISGVLMPTVLQALPDSLTQVIRKFAKQLDEWLKVALHDLPENLRNIKFELS 313

  Fly   707 SAFCQTLRRYTSLNHLAQAARAVLQNGAQISQMLSDLNRVDFHNVQEQAAWVSQCA----PAVVQ 767
            ..|.|.|:|.||||||.||:|.|:.:.....|||.|...||.:::.:|..:..:.:    ..::.
Zfish   314 RRFSQILKRQTSLNHLCQASRTVIHSADITFQMLEDWRNVDLNSITKQTLYTMEDSREDQRRLII 378

  Fly   768 RLESDFKAALQQQSSLEQWASWLQLVVESAMEEYNGK--PTYARAARQFLLKWSFYSSMIIRDLT 830
            :|..:|...|:.||.:|.:..||..:||..:....||  .:..|.|:||||.||.:.:.:|||:|
Zfish   379 QLYQEFDRLLEDQSPIEAYIEWLDSMVERCVVRVAGKRPGSLKRVAQQFLLMWSCFGTRVIRDMT 443

  Fly   831 LRSASSFGSFHLIRLLFDEYMFYLVEH-KIAEAQDKTAIAVICERMKKDMDFEFECQFAYITSDT 894
            |.||.||||||||.|:||:|:.||:|. ...|..::...|:..|....|...|.     .:.|.|
Zfish   444 LHSAPSFGSFHLIHLMFDDYVLYLLESLHCQERANELMRAMKGEGAPADTGEEL-----MLMSST 503

  Fly   895 EHQTTP---SSASSGGDVG 910
            ...|:|   |.|.|...||
Zfish   504 PTSTSPAPYSPAKSVHSVG 522

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rfx	NP_001247023.2	RFX_DNA_binding	392..468	CDD:460512	47/75 (63%)
rfx4	NP_991275.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	25..59		6/49 (12%)
		RFX_DNA_binding	58..136	CDD:460512	47/77 (61%)
		Necessary for dimerization	315..487		65/171 (38%)