Protein Alignment Mical and mical2b

DIOPT Version :10

• Sequence 1: NP_001247015.1
• Gene: Mical / 41225
• FlyBaseID: FBgn0053208
• Length: 4743
• Species: Drosophila melanogaster
• Sequence 2: XP_005174396.1
• Gene: mical2b / 569564
• ZFINID: ZDB-GENE-061207-15
• Length: 1679
• Species: Danio rerio
• Alignment Length: 2112
• Identity: 622/2112 - (29%)
• Similarity: 898/2112 - (42%)
• Gaps: 575/2112 - (27%)

  Fly    46 EHAAAAEAAELFDLLCVATTMRQILALHRAMCEAVGLRPSPLNDFYPRLKAKVRSWKAQALWKKF 110
            |....::|.:||:....|||.:..|.....:|..:.|.||....||..||..|..|||:.||.|.
Zfish     5 EEERTSQAGQLFENFIQATTCKGTLQAFSVLCRQLELNPSDHRGFYSSLKTAVTFWKAKGLWGKL 69

  Fly   111 DARAAHRVYGKGAACTGTRVLVIGAGPCGLRTAIEAQLLGAKVVVLEKRDRITRNNVLHLWPFVI 175
            |.||.|:.|.||.||..||.|:||.||||.|||||..||||||||:||||..:|||||||||:.|
Zfish    70 DKRAGHKEYSKGRACADTRCLIIGGGPCGFRTAIELALLGAKVVVIEKRDTFSRNNVLHLWPYTI 134

  Fly   176 TDLRNLGAKKFYGKFCAGSIDHISIRQLQCMLLKVALLLGVEIHEGVSFDHAVEP----SGDGGG 236
            .||||||||||||||||||||||||||||.||||:||::|||:|..|.|...:||    |.||.|
Zfish   135 HDLRNLGAKKFYGKFCAGSIDHISIRQLQLMLLKIALIVGVEVHVNVEFVKLLEPPEDQSTDGQG 199

  Fly   237 WRAAVTPADHPVSHYEFDVLIGADGKRNMLD-FRRKEFRGKLAIAITANFINKKTEAEAKVEEIS 300
            |||.:.|||:|||.|||||:|||||:|:.|| ||||||||||||||||||:|:.|.|||||||||
Zfish   200 WRAEIRPADNPVSDYEFDVIIGADGRRSTLDGFRRKEFRGKLAIAITANFVNRNTTAEAKVEEIS 264

  Fly   301 GVAFIFNQAFFKELYGKTGIDLENIVYYKDETHYFVMTAKKHSLIDKGVIIEDMADPGELLAPAN 365
            ||||||||.||.||..:|||||||||||||.||||||||||.||:||||||.|..:...|||..|
Zfish   265 GVAFIFNQKFFLELKEETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIINDYIETERLLAFDN 329

  Fly   366 VDTQKLHDYAREAAEFSTQYQMPNLEFAVNHYGKPDVAMFDFTSMFAAEMSCRVIVRKGARLMQC 430
            |:.:.|..||||||:|.|.||:|:|::|:||||:|||||||||.|:|:|.:..|..:...:|:..
Zfish   330 VNQEALLSYAREAADFGTNYQLPSLDYAINHYGQPDVAMFDFTCMYASENAALVREKSHRQLLVA 394

  Fly   431 LVGDSLLEPFWPTGSGCARGFLSSMDAAYAIKLWSNPQNSTLGVLAQRESIYRLLNQTTPDTLQR 495
            ||||||||||||.|:|||||||::.|.|:.||.|:..: ..|.:|::||||||||.|||.:.:.:
Zfish   395 LVGDSLLEPFWPMGTGCARGFLAAFDTAWMIKGWAQGK-EPLDLLSERESIYRLLPQTTAENISK 458

  Fly   496 DISAYTVDPATRYPNLNRESVNSWQVKHLV--DTDDPSILEQTFMDTHALQTPHLDTPGRRKRRS 558
            :...||:||||||||||...|...||:||.  ...|.|.||::.....::..         .||.
Zfish   459 NFEQYTIDPATRYPNLNSSCVRPHQVRHLFISGEQDLSSLERSGQTRRSVSI---------SRRE 514

  Fly   559 GDLLPQGATLLRWISAQLHSYQFIPELKEASDVFRNGRVLCALINRYRPDLIDYAATKDMSPVEC 623
            .::.|  ..||.|...|...|:.: .:.:.:..:|:|..|||||:|.||:|||:.:..:....:.
Zfish   515 SEVRP--GRLLLWCQNQTQDYRGV-NVTDLNTSWRSGLALCALIHRQRPELIDFDSLNEADCAKN 576

  Fly   624 NELSFAVLERELHIDRVMSAKQSLDLTELESRIWLNYLDQICDLFRGEIPHI----KHPKMDFSD 684
            |:|:|.|.|||..|..|.:.|:.......:..|.:.||.:..::|......:    |....:..|
Zfish   577 NQLAFDVAEREFGIQPVTTGKEMDAERGPDKLIMVLYLSKFYEMFHKSTQSVTGLPKEIDANNGD 641

  Fly   685 LRQK-----YR-INHTHAQ-PDFSKLL------ATKPKAKSPMQDAVDIPTTVQRRSVLEEERAK 736
            ...|     |. |||...: |...|.|      ..:.||.|..::.:...|........|::..|
Zfish   642 CSSKTANSLYNSINHARKRIPKLDKKLEESDVNRKRKKASSHHEELMSCQTAPPAGEREEQKENK 706

  Fly   737 RQRRHEQLLNIGGGAAGAAAGVAGSGTGTTTQGQNDTPRRSKKRRQV--DKTANILLLRTAL--C 797
            .:....|||          |....:......:.|:|:...|...|.|  |.|.|....|..:  .
Zfish   707 VRSMATQLL----------ARFEENAPSCALRRQSDSESDSDADRPVSLDLTENPRFARPKIEPT 761

  Fly   798 HEI------------------SNAISAFETIN-----EEERQQRLQEIEENRQERMSKRRQQRYH 839
            |..                  ||..|  ||::     |.:...||.||:...|          |.
Zfish   762 HPSTTPDKAKWQPSPYLRLLESNTRS--ETLHTEHYVESQSSHRLTEIQSECQ----------YS 814

  Fly   840 QTQNFYKSLQLLQAGKLLREGGEAGVAEDGTPFE-------------DYSIFLYRQQAPVFNDRV 891
            ...:.|||.:......|:             ||.             :..:...|::.|:....:
Zfish   815 SVSSAYKSSERRPRSPLI-------------PFTPTLSPMMHCLQQLEEQVIQQRKREPLNRKSI 866

  Fly   892 KDLERKL--LF---PDRERGD------IPSALPRTADEQF--------SDRIKNMEQRMTGRGGL 937
            |:..:||  ||   |.:.:.|      .||:.|:|..|..        :..:.:.:........|
Zfish   867 KERAQKLSSLFTGNPAQPQTDESSPAVSPSSPPQTIPESSTLSCLLSPAPSLTHKQCSEASETHL 931

  Fly   938 GGDKKPKDLMRAIGKIDSNDWNVREIEKKIELSKKTEIHGPKGREKVPKWSKEQFQARQHKMSKP 1002
            ..||..:  :|.:.::|.:        |:..:.|.:...|.|.......:..:      |:.:.|
Zfish   932 KADKHTE--IRRVERLDPS--------KQRTVGKVSSAIGVKAATLAILYETD------HRPNNP 980

  Fly  1003 QRQDSREAEKFKDIDQTIRNLDKQLKEGHNLDVGERGRNKVASIAGQFGKKDEANSDEKNAGSSN 1067
            ......||.:                      ..|.|   :.|:..:|
Zfish   981 ITLSLTEARR----------------------CAESG---LVSVRKEF----------------- 1003

  Fly  1068 ATTNTNNTVIPKSSSKVALAFKKQAASEKCRFCKQTVYLMEKTTVEGLVLHRNCLKCHHCHTNLR 1132
                        |:|        ...|:.|.||::.||:||:.:.||...||.|.:||.|..:||
Zfish  1004 ------------SAS--------LGGSDTCVFCQKRVYIMERLSAEGFFFHRECFRCHICGCSLR 1048

  Fly  1133 LGGYAFDRDDPQGRFYCTQHFRLPPKPLPQRTNKARKSAAAQPASPAVPPTAGSVPTAAAT-SEH 1196
            ||.:.|  |..||.|||..||.      .::|:...:....|.         |.:.:::.| |.|
Zfish  1049 LGAHTF--DSQQGTFYCKMHFS------QRKTSTRHRRGEIQD---------GGIRSSSITISNH 1096

  Fly  1197 MDTTPPRDQVDLLETSRANASADAMSDDEANVIDEHEWSGRNFLPESNNDSQSELSSSDESDTES 1261
            ..|...|.|.                            ||..|                :|.|:.
Zfish  1097 TSTDGTRGQP----------------------------SGGEF----------------DSSTQQ 1117

  Fly  1262 DSEMFEEADDSPFGAQTLQLASDWIGKQYCEDSDDSDDFYDSSEDDGKDDTEGEEFKKARELRRQ 1326
            |
Zfish  1118 D---------------------------------------------------------------- 1118

  Fly  1327 EVRLQPLPANLPTDTETEKLKLNVDNKENMA---DRSSLKSGNSFESARSQPSTPLSTPTR--VE 1386
               ||.||                |:||.::   .:.|.|..:..:||.:.|.:||....|  .:
Zfish  1119 ---LQTLP----------------DSKEIISVSEVKDSSKKADPADSAPACPDSPLQKVKRSTAK 1164

  Fly  1387 MEQLERNA--PRKFSSEIEAISEKLYHMNNMVKMNKDLEVLAKENLVKSDILRKLTLKEKWLAEN 1449
            .|...:|.  .:|..|.:..:..|.:|..   |.....||||:|:
Zfish  1165 GEITNKNILWKKKIRSTLPLVLMKKFHRG---KPEDKTEVLAEED-------------------- 1206

  Fly  1450 AAIAAGQKVTPTPSATAPGLQPKSKFDEKFEKVVS--PPQPVVEPKPKPVIDFN---LDELKPRK 1509
                                 ..|.|:|..|.:.|  |..|..:....|..|.:   |||: |:.
Zfish  1207 ---------------------GNSDFEEIHESLSSKKPSNPSTDSNCLPTKDNSSTPLDEI-PKI 1249

  Fly  1510 PNFEERPKEQLPRPESLKKPPQQKPKGSSTNVSRSNSLKSNASNGSPKVKKAPISNNSKMQIEGI 1574
            |.:......:.|:|.|      ..|:...|::|      |:..:.||| ||..:|.:.|   |.:
Zfish  1250 PLYRTHVLPEYPKPSS------SSPEPIVTSIS------SDPISFSPK-KKLTLSLSEK---EKL 1298

  Fly  1575 LGTLRKIQSQNSSDQDEDMDVDEDVERKPNKELNSKLKEIQASSFAGTMDHIKSQLTMPTVSAQA 1639
            |........::.:::.:...|      ||:..|..              ||.:.....|   ..|
Zfish  1299 LNWDLTNPGKSGAEEQQQQHV------KPSISLQH--------------DHPEPTHPQP---EPA 1340

  Fly  1640 PPSMDLSKYFPNQKQEKSSTSSTNKNQVTLKDVNLAKYFPSSPA---------PQRRTVETVADR 1695
            ||.....::..|.::..|..|    |.|.|:.....|..|.|..         .:.|....|.:.
Zfish  1341 PPLFGFQQWANNLRKSFSKGS----NPVVLRRNRPMKARPLSEGSFNVGAVFQDEERCGSLVDEG 1401

  Fly  1696 LKKSQTEAALAKTKLLEDQANNQAEKTKKEVEKEGESKKITKKVADSKAVPPKRQASLDTF-SLR 1759
            ..:.:||:.:|  .|||            :|....::.:.||.  |..::||::   |:.| |||
Zfish  1402 EARPRTESEIA--SLLE------------QVALGSKTSRGTKD--DMASLPPRK---LNFFSSLR 1447

  Fly  1760 EHQMDGALDLTKKKGP-----------TKASAGVKKPAKSGSTT------SVTKATATSKGKTIK 1807
            ..:::|| :.::.:|.           .||||..::..||.|::      .:|.:.|..|.|
Zfish  1448 IKRVEGA-EQSRGEGQKDILSILSRFRNKASAQQQQQQKSNSSSEDEQEPKLTHSGALQKKK--- 1508

  Fly  1808 IVKKIVPKGTKAKKAAEAAQESAVVEAPPEKKPPKDEAERILDEILGDGEYRSPSSEYQRLFQDE 1872
              :||..:.||:                       ||.:|:         :|:...:.|....:|
Zfish  1509 --EKIAIRQTKS-----------------------DELKRL---------HRAQVIQRQLEEVEE 1539

  Fly  1873 KSPSDLSDNIDRILEESGLDVELGLPKRSSKKLVKTKSLGEGDFD---MKPSKERLTGVQNILKR 1934
            |         .|.|||.|:.:|         |:::.::..:|..|   :..:..:|...:|.|.|
Zfish  1540 K---------QRSLEEKGVALE---------KVLRGENGDDGSTDEAALLQTWFKLVLEKNKLSR 1586

  Fly  1935 FESMSSVTSQNSDEQAAFKLRRMESTTSNLSSLTRSRESLVSVSDSMSDLEKTMDYLRN-----E 1994
            :||...:.:|..:         :|.|.|.|....|.|.:......|.|:|.:..:.|..     |
Zfish  1587 YESELMIFAQELE---------LEDTQSRLQQDLRRRMATEDCEKSASELVEEQNILVEIMKVVE 1642

  Fly  1995 WRNEATNFLQKKRDKFYAKKEEQEKESKILAK 2026
            .|::..:.|:::|.|  .|.|:::.||.||::
Zfish  1643 KRDKLVSLLEEQRLK--EKAEDRDLESLILSR 1672

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mical	NP_001247015.1	UbiH	128..>263	CDD:440419	101/138 (73%)
		CH_SF	567..669	CDD:469584	31/101 (31%)
		LIM_Mical	1097..1153	CDD:188823	26/55 (47%)
		PRK10819	<1450..>1557	CDD:236768	22/111 (20%)
		bMERB_dom	4592..4705	CDD:463467
mical2b	XP_005174396.1	UbiH	86..>229	CDD:440419	102/142 (72%)
		CH_MICAL2	514..623	CDD:409099	33/111 (30%)
		LIM_Mical	1013..1067	CDD:188823	26/55 (47%)
		PLN03029	<1308..1371	CDD:215544	16/89 (18%)
		bMERB_dom	1533..1665	CDD:463467	36/160 (23%)