Protein Alignment Mical and Mical2

DIOPT Version :10

• Sequence 1: NP_001247015.1
• Gene: Mical / 41225
• FlyBaseID: FBgn0053208
• Length: 4743
• Species: Drosophila melanogaster
• Sequence 2: XP_063125450.1
• Gene: Mical2 / 365352
• RGDID: 1311773
• Length: 1931
• Species: Rattus norvegicus
• Alignment Length: 2373
• Identity: 663/2373 - (27%)
• Similarity: 965/2373 - (40%)
• Gaps: 642/2373 - (27%)

  Fly    46 EHAAAAEAAELFDLLCVATTMRQILALHRAMCEAVGLRPSPLNDFYPRLKAKVRSWKAQALWKKF 110
            |....|:|:::|:....|||.:..|.....:...:.|.|....:||.:||:||.:|||:|||.|.
  Rat     5 EDEKQAQASQVFENFVQATTCKGTLQAFNILTCLLDLDPLDHRNFYTQLKSKVNTWKAKALWHKL 69

  Fly   111 DARAAHRVYGKGAACTGTRVLVIGAGPCGLRTAIEAQLLGAKVVVLEKRDRITRNNVLHLWPFVI 175
            |.|.:|:.|.:|.||:.|:.|::|.||||||||||...|||||||:||||..:||||||||||.|
  Rat    70 DKRGSHKEYKRGKACSNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDTFSRNNVLHLWPFTI 134

  Fly   176 TDLRNLGAKKFYGKFCAGSIDHISIRQLQCMLLKVALLLGVEIHEGVSFDHAVEPSGDGG----G 236
            .|||.||||||||||||||||||||||||.:|.||||:||||||..|.|....||..|..    |
  Rat   135 HDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEIHVNVEFVRVREPPKDQENRKIG 199

  Fly   237 WRAAVTPADHPVSHYEFDVLIGADGKRNMLD-FRRKEFRGKLAIAITANFINKKTEAEAKVEEIS 300
            |||...||||.:|::||||:|||||.||.|: ||||||||||||||||||||:.:.|||||||||
  Rat   200 WRAEFLPADHALSNFEFDVIIGADGHRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEIS 264

  Fly   301 GVAFIFNQAFFKELYGKTGIDLENIVYYKDETHYFVMTAKKHSLIDKGVIIEDMADPGELLAPAN 365
            ||||||||.||::|..:|||||||||||||.||||||||||.||:|||||:.|..|...||...|
  Rat   265 GVAFIFNQKFFQDLKEETGIDLENIVYYKDSTHYFVMTAKKQSLLDKGVILNDYIDTEMLLCAEN 329

  Fly   366 VDTQKLHDYAREAAEFSTQYQMPNLEFAVNHYGKPDVAMFDFTSMFAAEMSCRVIVRKGARLMQC 430
            |:...|..||||||:|:|.||:|:|:||:||.|:|||||||||||:|:|.:..:..|:..:|:..
  Rat   330 VNQDNLLSYAREAADFATNYQLPSLDFAINHNGQPDVAMFDFTSMYASENAALMRERQAHQLLVA 394

  Fly   431 LVGDSLLEPFWPTGSGCARGFLSSMDAAYAIKLWSNPQNSTLGVLAQRESIYRLLNQTTPDTLQR 495
            ||||||||||||.|:|||||||::.|.|:.:|.| :.....|.|||:|||:||||.||||:.:.:
  Rat   395 LVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSW-DQGTPPLEVLAERESLYRLLPQTTPENINK 458

  Fly   496 DISAYTVDPATRYPNLNRESVNSWQVKHLVDTDDPSILEQTFMDTHALQT-PHLDTPGRRKRRSG 559
            :...||:||||||||||...|...|||||..|.:        ||...|:. ..:.......||..
  Rat   459 NFEQYTLDPATRYPNLNVHCVRPHQVKHLYITKE--------MDRFPLERWGSVRRSASLSRRES 515

  Fly   560 DLLPQGATLLRWISAQLHSYQFIPELKEASDVFRNGRVLCALINRYRPDLIDYAATKDMSPVECN 624
            |:.|.  .||.|...|...||.: .:.:.:..:|:|..|||:|:.:||:||::.:..:...||.|
  Rat   516 DIRPN--KLLTWCQQQTKGYQHV-RVTDLTTSWRSGLALCAIIHSFRPELINFDSLNENDVVENN 577

  Fly   625 ELSFAVLERELHIDRVMSAKQSLDLTELESRIWLNYLDQICDLFRGEIPHIKHPKMDFSDLRQKY 689
            :|:|.|.:||..|..|.:.|:.....|.:....:.||.:..:||||.      |.......|:.|
  Rat   578 QLAFDVAKREFGILPVTTGKEMASTQEPDKLSMVMYLSKFYELFRGT------PLRPMDSRRKNY 636

  Fly   690 RIN------HTHAQPDFSKLLATKPKAKSPMQDAVDIPTTVQRRSVLEEERAKRQRRHEQLLNIG 748
            ..|      .|..|.::..|  |.|:.::|..||            ..||....:||.:...|:.
  Rat   637 GENADFGLGKTFFQNNYLNL--TLPRKRTPRVDA------------QTEENDVNKRRRQGFNNLE 687

  Fly   749 GGAAGAAAGVAGSGTGTTTQGQNDTPRRSKKRRQVDKTANILLLR--------TAL---CHEISN 802
            ...|.::..:..|         .:..:.|..:.:|...||.||.:        :||   |..:|.
  Rat   688 ELPAFSSRSLGSS---------QEYAKESGNQNKVKYMANQLLAKFEENTRNPSALKQDCPRVSG 743

  Fly   803 -----AISAFETINEEERQQRLQEIEENRQERMSKRRQQRYHQTQNFYKSLQLLQAGKLLREGGE 862
                 ..||    :.........::||: ..|:....::::..|         :..|::|||..:
  Rat   744 MGKPVLCSA----SRPPGTSHCPKLEES-TPRLPPPLKRQFSST---------VATGQVLRELNQ 794

  Fly   863 AGVAED--GTPFEDYSIFLYRQQAPVFND---------------------------RVKDLERKL 898
            ...:.:  |.|:          :|...:|                           |::.:|.|:
  Rat   795 VPASGECPGRPW----------RARAKSDLQLGGAENLATLPPTCQGALALSGVLRRLQQVEEKV 849

  Fly   899 LFPDRERGDIPSALPRTADEQFSDRIKNMEQR------MTGRGGLGGDKKPKDLMRAIGKIDSND 957
            |   ::|..      ..|:.:|  ..||::::      |.|.|.|..||.
  Rat   850 L---QKRAQ------NLANREF--HTKNIKEKAAHLASMFGHGDLPQDKL--------------- 888

  Fly   958 WNVREIEKKIELSKKT-EIHGPKGREKVPKWSKEQFQARQHKMSKPQRQDSREAEKFKDIDQTIR 1021
                       |||:. ..|.|.....:|  |.:...|     ..|...||
  Rat   889 -----------LSKRVPHAHPPSPPSCLP--SPDPAAA-----PSPPAADS-------------- 921

  Fly  1022 NLDKQLKEGHNLDVGERGRNKVASIAGQFGK------------KDEANSDEKNAGSSNATTNTNN 1074
                 :.....|.||     ||:|..|...:            |.:|.|.:..:
  Rat   922 -----VSPARKLTVG-----KVSSGIGAAAEVLVNLYLNDHRPKTQATSPDLES----------- 965

  Fly  1075 TVIPKSSSKVALAFKKQAASEKCRFCKQTVYLMEKTTVEGLVLHRNCLKCHHCHTNLRLGGYAFD 1139
              :.|:...::|     ...:.|.|||:.||:||:.:.||...||.|.:|..|...||:..||||
  Rat   966 --LRKAEFPLSL-----GGRDTCYFCKKRVYVMERLSAEGHFFHRECFRCSVCAAILRVAAYAFD 1023

  Fly  1140 RDDPQGRFYCTQHF---RLPPKPLPQRT--NKARKSAAAQPASPA----VPPTAGSVPTAAATSE 1195
            .|  :|:|||..||   :...|...:|.  |:.|:.....|...|    ||..:.....|.:|.|
  Rat  1024 CD--EGKFYCKLHFAHCKTSSKQRKRRAELNQQREEEGTWPEQEAARRDVPAESSCAVAAISTPE 1086

  Fly  1196 HMDTTP---------PRDQVDLLETSRANASADAMSDDEANVIDEHEWSGRNFLPESNNDSQSEL 1251
            .....|         |:....:.:.:......:|.|...:      ||:.....|  ..|.|..|
  Rat  1087 GSPPGPILLADEPISPKKSKSVPKPNSRPMEVEATSPRPS------EWTSVRIGP--GQDGQDVL 1143

  Fly  1252 -------SSSDESDTESDSEMFEEADDSPFGAQTLQLASDWIGKQYCEDSDDSDDFYDSSEDDGK 1309
                   |....|||||||       .|..|..|          :.||:..
  Rat  1144 AVRVLVTSEDSSSDTESDS-------GSIIGPCT----------EACEERP-------------- 1177

  Fly  1310 DDTEGEEFKKARELRRQEVRLQPLPANLP-TDTETEKLKLNVDNKENMA----DRS-SLKSGNSF 1368
                        .|.......|||..::. .:|.|:.:.|.:.:||..|    .|: ||:|.:.:
  Rat  1178 ------------RLPESPPLSQPLTRHISLRETLTQPVSLLLHHKEPQAVPGLQRAYSLQSPSKY 1230

  Fly  1369 ESARSQPSTPLSTPTRVEMEQLERNAPRKFSSEIEAISEKLYHMNNMVKMNKDLEVLAKENLVKS 1433
            ::.|                       |||.|             |...||:
  Rat  1231 QNWR-----------------------RKFQS-------------NSTPMNQ------------- 1246

  Fly  1434 DILRKLTLKEKWLAENAAIAAGQKVTPTPSATAPGLQPKSKFDEKFEKVVSPPQPVVEPKPKPVI 1498
                            .|::..::..|:.|:::|.| |.|     |.....|.....:.....|.
  Rat  1247 ----------------RALSPPKEPPPSSSSSSPSL-PSS-----FSSASVPGHTTDDSSSPQVP 1289

  Fly  1499 DFNL-------DELKPRKPNFEERPKEQ------------------------LPRPESLKKPPQ- 1531
            .:||       |::.| .|.:..|.:.|                        .|..|.|:.|.:
  Rat  1290 AYNLHSPQISRDDVSP-TPIYLRRARAQGITKEIPLYLPHSPMLESTEHCLVSPDGEELRSPEEI 1353

  Fly  1532 QKPKGSSTNVSRSNSLKSNASNGSPKVKKAPISNNSKMQIEGILGTLRKIQSQNSSDQDEDMDVD 1596
            ....|....::..|| :|...:..|...|.|...|..:.: |..|        |:.|..|:..:.
  Rat  1354 SASDGCQKALALGNS-ESTHKDSYPVSGKDPYLPNQMLAL-GAAG--------NTGDLSEESRMG 1408

  Fly  1597 EDVERKPNKELNSKLKEIQASSFAGTMDHIKSQLTMPTVSAQAPPSMDLSKYFPNQK------QE 1655
            :....:.:||....||::..:         :.|.||         .:|.:.|....|      ||
  Rat  1409 QTGGAELSKERKLGLKKLVLT---------EEQKTM---------LLDWNDYTQEHKAGERLAQE 1455

  Fly  1656 KSSTSSTNKNQVTLKDVNLAKYFPSSPAPQRRTVETVADRLKKSQTEAALAKTKLLEDQANNQAE 1720
            |:.....|    :||.:                         .|.|.:...|.|||..
  Rat  1456 KAENGRGN----SLKPI-------------------------CSSTLSQAVKEKLLSQ------- 1484

  Fly  1721 KTKKEVEKEGESKKITKKVADSKAVPPKRQASLDTFSLREHQMDGALDLTKKKGPTKASAGVKKP 1785
              ||.:   ||::....|....:.|||                        .|.|.:..|..
  Rat  1485 --KKAL---GETRTPAAKAPREREVPP------------------------PKSPLRLIANA--- 1517

  Fly  1786 AKSGSTTSVTKATATSKGKTIKIVKKIVPKGTKAKKAAEAAQESAVVEAPPEKKPPKDEAERILD 1850
                                  |.:.::|.....||.:         ..|..|..|:.:......
  Rat  1518 ----------------------IFRSLLPSSEAGKKTS---------SKPETKTLPRGQPHAFTR 1551

  Fly  1851 EI----LG---DGEYRSPSSEYQR---LFQDEKSP-------SDLSDNIDRILEESGLDVELGLP 1898
            ..    ||   ||:.:||.....:   .|....||       ||||.....:...|       ||
  Rat  1552 SFSFRKLGSSKDGDQQSPGRHMAKKASAFFSLASPTSKAAQASDLSPPNPILRSRS-------LP 1609

  Fly  1899 KRSSKKLVKTKSLGEGDFDMKPSKERLTGVQNILKRFESMSSVTSQNSDEQAAFKLRRMESTTSN 1963
            .|.||....|.||        |...::..|..:|::.          |.:.||...::..|..|.
  Rat  1610 NRPSKMFFATTSL--------PPSSKVEDVPTLLEKV----------SLQDAAQGPKKGASHISP 1656

  Fly  1964 LSSLTRSRESLVSVSDSMSDLEKTMDYLRNEWRNEATNFLQKKRDKFYAKKEEQEKESKILAKPD 2028
            |....:|.||.:.......|:                      .|.|.:.||:....:::    .
  Rat  1657 LGLKDKSFESFLQECKERKDI----------------------GDFFNSPKEKGPPGNRV----P 1695

  Fly  2029 PLDNLPVQYRDSKLAKFFGLAASKSPENRK-SPI-KKKKSPSKTPKVTKANNSLEELAKISNVRQ 2091
            .|:.| ||..||.........:|...:.|. :|: :...||:.:.........|....|....|:
  Rat  1696 SLEKL-VQPVDSTSMGQVAHPSSTGQDARPGAPVTEDTSSPTSSSAEEDVETQLSSRLKEKIPRR 1759

  Fly  2092 TKKAQPKTLKPVEVKPLKPASPVPDDFEILDLLEKATEAKELERSKT-KSPAVESISQTPKEAIV 2155
            .:|.:.:..|..|:|.|..|..:....|.::..::.:|.:.:...|. :....:|.:|...:.:.
  Rat  1760 RRKLEKQMAKQEELKRLHKAQAIQRQLEEVEERQRTSEIQGVRLEKVLRGETADSGTQDEAQLLQ 1824

  Fly  2156 E-ISLPVEDIKNLPKTGCDKSSNSSRRGSQSSLIMSR----RHSEISLNEKLNQDALAALNQIEK 2215
            | ..|.:|             .|...|.....|||::    ...:..|.:||.|..|....| :.
  Rat  1825 EWFKLVLE-------------KNKLMRYESELLIMAQELELEDHQSRLEQKLRQKMLKDEGQ-KD 1875

  Fly  2216 EREAEQVDELFQSMVEEMEQEPQPTAIVEPPEE 2248
            |.:.::..|:|:.|::.:||.   ..:|:..||
  Rat  1876 ENDLKEEQEIFEEMMQVIEQR---NKLVDSLEE 1905

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mical	NP_001247015.1	UbiH	128..>263	CDD:440419	96/138 (70%)
		CH_SF	567..669	CDD:469584	31/101 (31%)
		LIM_Mical	1097..1153	CDD:188823	26/55 (47%)
		PRK10819	<1450..>1557	CDD:236768	25/138 (18%)
		bMERB_dom	4592..4705	CDD:463467
Mical2	XP_063125450.1	None