Protein Alignment topi and gli3

DIOPT Version :10

• Sequence 1: NP_649945.1
• Gene: topi / 41199
• FlyBaseID: FBgn0037751
• Length: 814
• Species: Drosophila melanogaster
• Sequence 2: NP_991291.1
• Gene: gli3 / 403042
• ZFINID: ZDB-GENE-041111-162
• Length: 1553
• Species: Danio rerio
• Alignment Length: 710
• Identity: 143/710 - (20%)
• Similarity: 230/710 - (32%)
• Gaps: 231/710 - (32%)

  Fly   170 KPHLVPTVSAPPAPLSKPASERV--IRENQVR------------LRRYIKDEMKY--DLATGIES 218
            :|..||::..|||..:.|....:  ||.:..|            ...||...|.|  .|.:....
Zfish   150 EPSPVPSLHMPPALAASPTFSDISLIRISPHRNPSMGTDSPFNPSHPYINPYMDYIRSLHSSPSL 214

  Fly   219 SRKNAAKG-----PNECTMCDRKFVHASG-LVRHMEKHALDLIPSQTSEQPHTIPA-AGLHV-VV 275
            |..:||:|     .:...:...::.|... ||.|...:| |||||..|.   |.|| :.||: .:
Zfish   215 SMVSAARGLSPADTHHTGLTTAEYYHQMALLVGHRSPYA-DLIPSVGST---TGPASSALHMEYL 275

  Fly   276 KCNSCGRIFYDPQVAFRHGLIHDSEHSTMRQSPMTQVPSNRADFNELLLDGEMLIDNDP-AFAT- 338
            :.....| |..|::..|          ..|:.|:...||    .::...|.:.:|...| :..|
Zfish   276 QAMESSR-FPSPRLINR----------PSRKRPIPNSPS----LSDPSFDLQAMIRTSPNSLVTI 325

  Fly   339 -SNQNTNPPKKEMFSSLILGSVLQCEFCEYIFADIA-----EL-----------------LVHSA 380
             :|..::......:..|..|::.......|....:|     :|                 |:|||
Zfish   326 LNNSRSSSSNSGSYGHLSAGTISPALSFAYPPTPVALQMHQQLISRQPGIVGSAFGHSPPLMHSA 390

  Fly   381 --------------SHVAERRFECTACDIQMNTAKEASIHFQTD-------------------CI 412
                          |.:|.......:.|.|.....|:::....|                   .:
Zfish   391 LAFSTQRPVPAIQTSSLAPTERSIVSNDSQSKPTSESAVSSTGDPMNHKRSKLKPEEEPPSPRAV 455

  Fly   413 FMREAIRSLNVTLSRYFVCNVCELKFANTDLLQEHRCTSFHYFPRLNENGKKLLLPCDFCDVNFE 477
            .|:|.:..:.:....    ...|......:::.|..|   |:               |.|...|:
Zfish   456 SMQEQMNGMTLVKEE----GDKEEGKQEPEVVYETNC---HW---------------DGCCREFD 498

  Fly   478 FAHDFLAHSEEKHLNKKKREKETRNTGAGRIRQYLC--DICG---KSYTQSSHLWQHLRFHQGVK 537
            .....:.|....|::.:|:|             ::|  :.|.   |.:.....|..|:|.|.|.|
Zfish   499 TQEQLVHHINNDHIHGEKKE-------------FVCRWEECSREQKPFKAQYMLVVHMRRHTGEK 550

  Fly   538 PFVCQEENCDRKFTIRPDLNDHIRKCHTGERPYLCLVCGKRFLTGSVFYQHRLIHRGERRYECEE 602
            |..|..|.|.:.::...:|..|:|. ||||:||:|                          |.|.
Zfish   551 PHKCTFEGCSKAYSRLENLKTHLRS-HTGEKPYVC--------------------------EHEG 588

  Fly   603 CGKRFYRA-DALKNHQRIHTGEKPYSCLF--CTKTFRQRGDRDKHIRARH---SHLDANSRLMMQ 661
            |.|.|..| |..|:..|.|:.||||.|..  |||.:.......||::..|   :|:
Zfish   589 CNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHV--------- 644

  Fly   662 MQKFQLETAAAQKAQSHNPEQQDNDVAGGASTSDVPSGSGFMSTEPSVAEMQYSITPEQQEEMVC 726
            .:|.:.||......|...|         ||:|.                :.:|:....:|:|  |
Zfish   645 TKKQRGETYPRPPPQPREP---------GATTD----------------QKEYTHATSKQDE--C 682

  Fly   727 VPIDEVNNSFFMSHYMQAVPMEEDGSGQHIIVFEQPGQNMDMMSIYDQQQVGEPMHESGV 786
            :.:..:...         .||....|         ||......|   .|.:..|....||
Zfish   683 LQVKSIKTE---------KPMTSQSS---------PGSQSSCSS---DQSINGPHVSQGV 721

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
topi	NP_649945.1	C2H2 Zn finger	230..250	CDD:275370	4/20 (20%)
		C2H2 Zn finger	277..297	CDD:275370	4/19 (21%)
		C2H2 Zn finger	431..453	CDD:275368	3/21 (14%)
		COG5048	<450..647	CDD:227381	51/204 (25%)
		C2H2 Zn finger	469..490	CDD:275368	4/20 (20%)
		C2H2 Zn finger	513..564	CDD:275368	16/55 (29%)
		C2H2 Zn finger	541..561	CDD:275368	5/19 (26%)
		C2H2 Zn finger	572..592	CDD:275368	1/19 (5%)
		C2H2 Zn finger	600..620	CDD:275368	8/20 (40%)
		zf-H2C2_2	612..637	CDD:463886	11/26 (42%)
		C2H2 Zn finger	628..646	CDD:275368	6/19 (32%)
gli3	NP_991291.1	FOXP-CC	488..>522	CDD:465036	9/64 (14%)
		C2H2 Zn finger	524..546	CDD:275368	5/21 (24%)
		SFP1	<548..638	CDD:227516	36/116 (31%)
		C2H2 Zn finger	554..576	CDD:275368	6/22 (27%)
		C2H2 Zn finger	584..607	CDD:275368	10/48 (21%)
		C2H2 Zn finger	615..636	CDD:275368	6/20 (30%)