Protein Alignment topi and Gli2

DIOPT Version :10

• Sequence 1: NP_649945.1
• Gene: topi / 41199
• FlyBaseID: FBgn0037751
• Length: 814
• Species: Drosophila melanogaster
• Sequence 2: NP_001100639.2
• Gene: Gli2 / 304729
• RGDID: 1309270
• Length: 1544
• Species: Rattus norvegicus
• Alignment Length: 841
• Identity: 169/841 - (20%)
• Similarity: 270/841 - (32%)
• Gaps: 321/841 - (38%)

  Fly     5 VSGEYTLAEIEVELDQQLTPDDLQPGAT---------------VLATNESTGGLERI-------- 46
            :||...:::|.:   .:|:|....||.:               .|.:..|:..|..|
  Rat    88 LSGSPVISDISL---IRLSPHPAGPGESPFSAHHPYMNPHMEHYLRSVHSSPTLSMISAARGLSP 149

  Fly    47 --VSHEELSR--FFAVGPAGALPMPTDVVVERTL--ADPA-FKQILQEADGKKGFDPQAEQMKIR 104
              |:||.|..  .|::...|.  .|:|...:.||  :.|. :..||.::.|      .|....:.
  Rat   150 ADVAHEHLKERGLFSLAAPGT--NPSDYYHQMTLMASHPTPYGDILMQSGG------AASAPHLH 206

  Fly   105 DFLAGVTSSKMTTEQSVFHGSRSNSSASTVNRIKCPTCLVQFDAVAFQNHSCEAKPIEVAVPQQE 169
            |:|..|.:|:.::
  Rat   207 DYLNPVDASRFSS---------------------------------------------------- 219

  Fly   170 KPHLVPTVSAPPAPLSKPASERVIRENQVRLRRYIKDEMKYDLATGIESSRKNAAKGPNECTMCD 234
             |.:.|.:|...|....|.|:.     .:.|:|.|:.... .|...|.:||.::|
  Rat   220 -PRVTPRLSRKRALSISPLSDA-----SLDLQRMIRTSPN-SLVAYINNSRSSSA---------- 267

  Fly   235 RKFVHASGLVRHMEKHALDLIPSQTSEQPHTI-PAAGLHVVVKCNSCGRIFYDPQVAFRH--GLI 296
                 |||...|:...||.  |:.|.  ||.| |.|...::.:....|.       ||.|  .||
  Rat   268 -----ASGSYGHLSAGALS--PAFTF--PHPINPVAYQQILSQQRGLGS-------AFGHTPPLI 316

  Fly   297 HDSEHSTMRQSPM--------TQVPSNRADFNELLLDGEMLIDNDPAFATSNQNTNPPKKEMFSS 353
            ..|. :.:.|.||        ||:.||.::                ..:.:|||....:..:.|:
  Rat   317 QPSP-TFLAQQPMTLTSITVPTQLGSNSSN----------------CISDANQNKQNSESAVSST 364

  Fly   354 LILGSVLQCEFCEYIFADIAELLVHSASHVAERRFECTACDIQMNTAKEASIHFQTDCIFMREAI 418
                              :..:.:|..|.|                        :|:...:|.|
  Rat   365 ------------------VNPITIHKRSKV------------------------KTEAEGLRPA- 386

  Fly   419 RSLNVTLSRYFVCNVCELK--FANTDLLQEHRC----TSFHYFPRLNENGKKLLLPCDFCDVNFE 477
            ..|.:|..:     :.:||  ....|..||...    |:.|:..               |...::
  Rat   387 SPLGLTQEQ-----LADLKEDLDRDDCKQEAEVVIYETNCHWAD---------------CTKEYD 431

  Fly   478 FAHDFLAHSEEKHLNKKKREKETRNTGAGRIRQYLC--DIC---GKSYTQSSHLWQHLRFHQGVK 537
            .....:.|...:|::.:|:|             ::|  ..|   .|.:.....|..|:|.|.|.|
  Rat   432 TQEQLVHHINNEHIHGEKKE-------------FVCRWQACTREQKPFKAQYMLVVHMRRHTGEK 483

  Fly   538 PFVCQEENCDRKFTIRPDLNDHIRKCHTGERPYLCLVCGKRFLTGSVFYQHRLIHRGERRYECEE 602
            |..|..|.|.:.::...:|..|:|. ||||:||:|                          |.|.
  Rat   484 PHKCTFEGCSKAYSRLENLKTHLRS-HTGEKPYVC--------------------------EHEG 521

  Fly   603 CGKRFYRA-DALKNHQRIHTGEKPYSCLF--CTKTFRQRGDRDKHIRARH---SHLDANSRLMMQ 661
            |.|.|..| |..|:..|.|:.||||.|..  |||.:.......||::..|   :|:....|..:.
  Rat   522 CNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTVHGPDAHVTKKQRNDVH 586

  Fly   662 MQKFQLETAAAQKAQSHNPEQQDNDVAGGASTSDVPSGSGFMSTEPSVAEMQYSITPEQQEEMVC 726
            ::...|:            |..||:  .||.    |.|.|   .|.||.....|.|.|.     |
  Rat   587 LRAPLLK------------ENGDNE--AGAE----PGGRG---PEESVEASSTSHTAED-----C 625

  Fly   727 VPIDEVNNSFFMSHYMQAVPMEEDGSGQHIIVFEQPGQNMDMMSIYDQQQVGE-PMHESGV 786
            :             :::|:..|..|..|     ..||......|  :...:|. |.::|||
  Rat   626 L-------------HIKAIKTESSGLCQ-----SSPGAQSSCSS--EPSPLGSVPNNDSGV 666

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
topi	NP_649945.1	C2H2 Zn finger	230..250	CDD:275370	4/19 (21%)
		C2H2 Zn finger	277..297	CDD:275370	5/21 (24%)
		C2H2 Zn finger	431..453	CDD:275368	6/27 (22%)
		COG5048	<450..647	CDD:227381	50/204 (25%)
		C2H2 Zn finger	469..490	CDD:275368	2/20 (10%)
		C2H2 Zn finger	513..564	CDD:275368	16/55 (29%)
		C2H2 Zn finger	541..561	CDD:275368	5/19 (26%)
		C2H2 Zn finger	572..592	CDD:275368	1/19 (5%)
		C2H2 Zn finger	600..620	CDD:275368	8/20 (40%)
		zf-H2C2_2	612..637	CDD:463886	11/26 (42%)
		C2H2 Zn finger	628..646	CDD:275368	6/19 (32%)
Gli2	NP_001100639.2	SFP1	<481..571	CDD:227516	36/116 (31%)
		C2H2 Zn finger	487..509	CDD:275368	6/22 (27%)
		C2H2 Zn finger	517..540	CDD:275368	10/48 (21%)
		C2H2 Zn finger	548..569	CDD:275368	6/20 (30%)