Protein Alignment topi and GLI2

DIOPT Version :10

• Sequence 1: NP_649945.1
• Gene: topi / 41199
• FlyBaseID: FBgn0037751
• Length: 814
• Species: Drosophila melanogaster
• Sequence 2: XP_054197371.1
• Gene: GLI2 / 2736
• HGNCID: 4318
• Length: 1670
• Species: Homo sapiens
• Alignment Length: 729
• Identity: 157/729 - (21%)
• Similarity: 241/729 - (33%)
• Gaps: 214/729 - (29%)

  Fly    84 ILQEADGKKGFDPQAEQMKIRDFLAGVTSSKMTTEQSVFHGSRSNSSASTVNRIKCP--TCLVQF 146
            ::..|.|....|...|.:|.|. |.|:.:.. ||....:|      ..:.|.....|  ..|:|.
Human   225 MISAARGLSPADVAQEHLKERG-LFGLPAPG-TTPSDYYH------QMTLVAGHPAPYGDLLMQS 281

  Fly   147 DAVAFQNHSCE-AKPIEVAVPQQEKPHLVPTVSAPPAPLSKPASERVIRENQVRLRRYIKDEMKY 210
            ...|...|..: ..|::|:  :...|.:.|.:|...|....|.|:.     .:.|:|.|:....
Human   282 GGAASAPHLHDYLNPVDVS--RFSSPRVTPRLSRKRALSISPLSDA-----SLDLQRMIRTSPN- 338

  Fly   211 DLATGIESSRKNAAKGPNECTMCDRKFVHASGLVRHMEKHALDLIPSQTSEQPHTI-PAAGLHVV 274
            .|...|.:||.::|               |||...|:...||.  |:.|.  ||.| |.|...::
Human   339 SLVAYINNSRSSSA---------------ASGSYGHLSAGALS--PAFTF--PHPINPVAYQQIL 384

  Fly   275 VKCNSCGRIFYDPQVAFRH--GLIHDSEHSTMRQSPMTQVPSNRADFNELLLDGEMLIDNDPAFA 337
            .:....|.       ||.|  .||..|. :.:.|.||. :.|..|...:|......|.|
Human   385 SQQRGLGS-------AFGHTPPLIQPSP-TFLAQQPMA-LTSINATPTQLSSSSNCLSD------ 434

  Fly   338 TSNQNTNPPKKEMFSSLILGSVLQCEFCEYIFADIAELLVHSASHVAERRFECTACDIQMNTAKE 402
             :|||....:..:.|:                  :..:.:|..|.|...............|..:
Human   435 -TNQNKQSSESAVSST------------------VNPVAIHKRSKVKTEPEGLRPASPLALTQGQ 480

  Fly   403 ASIHFQTDCI--FMREAIRSLNVTLSRYFVCNVCELKFANTDLLQEHRC----TSFHYFPRLNEN 461
            .|.|....|.  ..:|.:..|...|.|             .|..||...    |:.|:
Human   481 VSGHGSCGCALPLSQEQLADLKEDLDR-------------DDCKQEAEVVIYETNCHW------- 525

  Fly   462 GKKLLLPCDFCDVNFEFAHDFLAHSEEKHLNKKKREKETRNTGAGRIRQYLC--DIC---GKSYT 521
                    :.|...::.....:.|...:|::.:|:|             ::|  ..|   .|.:.
Human   526 --------EDCTKEYDTQEQLVHHINNEHIHGEKKE-------------FVCRWQACTREQKPFK 569

  Fly   522 QSSHLWQHLRFHQGVKPFVCQEENCDRKFTIRPDLNDHIRKCHTGERPYLCLVCGKRFLTGSVFY 586
            ....|..|:|.|.|.||..|..|.|.:.::...:|..|:|. ||||:||:|
Human   570 AQYMLVVHMRRHTGEKPHKCTFEGCSKAYSRLENLKTHLRS-HTGEKPYVC-------------- 619

  Fly   587 QHRLIHRGERRYECEECGKRFYRA-DALKNHQRIHTGEKPYSCLF--CTKTFRQRGDRDKHIRAR 648
                        |.|.|.|.|..| |..|:..|.|:.||||.|..  |||.:.......||::..
Human   620 ------------EHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSSLRKHVKTV 672

  Fly   649 HSHLDANSRLMMQMQKFQLETAAAQKAQSHNPEQQDNDVAGGASTSDVP----SGSGFMSTEPSV 709
            |.                        ..:|..::|.|||     ....|    :|.....|||..
Human   673 HG------------------------PDAHVTKKQRNDV-----HLRTPLLKENGDSEAGTEPGG 708

  Fly   710 AE-MQYSITPEQQEEMVCVPIDEVNNSFFMSHYMQAVPMEEDGSGQHIIVFEQPGQNMDMMSIYD 773
            .| .:.|.|.:..|:  |:             :::|:..|..|..|     ..||......|  :
Human   709 PESTEASSTSQAVED--CL-------------HVRAIKTESSGLCQ-----SSPGAQSSCSS--E 751

  Fly   774 QQQVGE-PMHESGV 786
            ...:|. |.::|||
Human   752 PSPLGSAPNNDSGV 765

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
topi	NP_649945.1	C2H2 Zn finger	230..250	CDD:275370	4/19 (21%)
		C2H2 Zn finger	277..297	CDD:275370	5/21 (24%)
		C2H2 Zn finger	431..453	CDD:275368	4/25 (16%)
		COG5048	<450..647	CDD:227381	50/204 (25%)
		C2H2 Zn finger	469..490	CDD:275368	2/20 (10%)
		C2H2 Zn finger	513..564	CDD:275368	16/55 (29%)
		C2H2 Zn finger	541..561	CDD:275368	5/19 (26%)
		C2H2 Zn finger	572..592	CDD:275368	1/19 (5%)
		C2H2 Zn finger	600..620	CDD:275368	8/20 (40%)
		zf-H2C2_2	612..637	CDD:463886	11/26 (42%)
		C2H2 Zn finger	628..646	CDD:275368	6/19 (32%)
GLI2	XP_054197371.1	PRK12323	<19..166	CDD:481241
		SFP1	<583..673	CDD:227516	36/116 (31%)
		C2H2 Zn finger	589..611	CDD:275368	6/22 (27%)
		C2H2 Zn finger	619..642	CDD:275368	10/48 (21%)
		C2H2 Zn finger	650..671	CDD:275368	6/20 (30%)
		PHA03247	<996..1473	CDD:223021