DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment hyd and Magi3

DIOPT Version :9

Sequence 1:	NP_001287262.1	Gene:	hyd / 41181	FlyBaseID:	FBgn0002431	Length:	2887	Species:	Drosophila melanogaster
Sequence 2:	NP_001152826.1	Gene:	Magi3 / 99470	MGIID:	1923484	Length:	1476	Species:	Mus musculus

Alignment Length:	1386	Identity:	246/1386 - (17%)
Similarity:	400/1386 - (28%)	Gaps:	539/1386 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly  1543 PVRLGVVRPTAPFTMSSSNLDVRN-------------------SDDLF----SVDPLAPSNVESP 1584
            |:||..|:|....     |.|:|:                   .|:|:    .....||.:.|.|
Mouse    99 PIRLKTVKPGKVI-----NKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVP 158

  Fly  1585 SEQILVHDAGNDQSANFNIQQNYDVVAMETIRDASESEEVINREANSHNQDDELIENQR---NED 1646
                     |.|          |:.:::|..:...||..::    .|...|.......:   ...
Mouse   159 ---------GVD----------YNFISVEQFKALEESGALL----ESGTYDGNFYGTPKPPAEPS 200

  Fly  1647 GMQDDESDNDFTFNDAETESDSDDNQSNQEVQRSVQAGATVGSENDIGVLFLEDESGDSSAQEED 1711
            ..|.|..|.....|:.:|||......|..:::|.                       |||..||:
Mouse   201 PFQPDPVDQVLFDNEFDTESQRKRTTSVSKMERM-----------------------DSSLPEEE 242

  Fly  1712 GSEDGESDDQSDEFNFNEQQLER-----------RSTNSN--------ARSDLAPQTMQWAIRSR 1757
            ..||.|:.:.|......|...|.           ..|||:        ...:|.|....|.:...
Mouse   243 EDEDKEAVNGSGSMETREMHSETSDCWMKTVPSYNQTNSSMDFRNYMMRDENLEPLPKNWEMAYT 307

  Fly  1758 DTARSSVRVPTGSNMVFIDPMALRRSTVP-----------------------------ASTTVTT 1793
            ||..............::||...:::..|                             ..|....
Mouse   308 DTGMIYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFEN 372

  Fly  1794 P-----------SIEPHTMATTASNLARAFGITIRQISELISILSYNVLNDIETSLKIQNDEAIA 1847
            |           ..|.|:..|   ::.||.  ..|..|:|..:|       :..|||
Mouse   373 PVEEAKRKKQLGQAEIHSAKT---DVERAH--FTRDPSQLKGVL-------VRASLK-------- 417

  Fly  1848 VQAFVEKRLKATWDWMFTVMDGTEAQLKFGAYLTNYTDPNHPLHPLNLSAQASSSQTPAPATSS- 1911
                     |:|..:.||::.|..              |:..|...|:.....::|....|...
Mouse   418 ---------KSTMGFGFTIIGGDR--------------PDEFLQVKNVLKDGPAAQDGKIAPGDV 459

  Fly  1912 --SVNGVNIMGSNSRRDFFTYCLSLMRSHTSEHRDALPVLDITALRHIAYVLDAFVYYMRNDSGF 1974
              .:||..::|                 ||  |.|.:.:..:..:.....:.....|.:.:||
Mouse   460 IVDINGNCVLG-----------------HT--HADVVQMFQLVPVNQYVNLTLCRGYPLPDDS-- 503

  Fly  1975 YDKQDTISGRINNLSPMT--ESYDTDDELANLEEFNADV----------QMSASSMPSG-SQGTR 2026
               :|.:.. |...:|:.  :|....:...|.::|....          |:.||...:| |:.:.
Mouse   504 ---EDPVVD-IVAATPVINGQSLTKGETCMNTQDFKLGAMVLDQNGKSGQILASDRLNGPSESSE 564

  Fly  2027 RHAFFARSESTLSLGCSAPEGFELPL-----DMAMPLADKP--------------HLLQPNSKRQ 2072
            :.|..|.|      |.|.||...:||     .....:||.|              ..||.....:
Mouse   565 QRASLASS------GSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIK 623

  Fly  2073 ELF----------------------ANLPLLV--------TTNANNSGATNDGDGG--SIFDYTP 2105
            |::                      |::|||:        |..|.....|.:..|.  :|.:..|
Mouse   624 EIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPCSPTKTAKTKTDTKENSGSLETINEPIP 688

  Fly  2106 TRLGFSNSLKR--------------NERVYETVP---------IDSSKTGDGNVTNKAEGSTDSN 2147
            ..:.|..|:.|              ::.:||..|         :...::|.|......:| .|.:
Mouse   689 QPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDG-PDQS 752

  Fly  2148 IYV------QLKKKQG----SDDF----------KSHKEADGNQSKYEKVVLMET---------- 2182
            ||:      ...:|.|    :|:.          ||||:.         :.||.|
Mouse   753 IYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQV---------LDLMTTAARNGHVLLT 808

  Fly  2183 -------------DDS----------------LPSTSKSTEALMAT--RPE-----VIIAPNKAS 2211
                         |:|                ||:.|...||...|  |.|     .:|..:|:.
Mouse   809 VRRKIFYGEKQPEDESHQAFSQNGSPRLNRAELPTRSAPQEAYDVTLQRKENEGFGFVILTSKSK 873

  Fly  2212 VSPATAARSVIVLAGGS----C--LKTID--SDINNYSASNLS-------------------TAE 2249
            ..|......:..:..||    |  ||..|  |.:|..|..:||                   .||
Mouse   874 PPPGVIPHKIGRVIDGSPADRCGGLKVGDHISAVNGQSIVDLSHDNIVQLIKDAGVTVTLTVVAE 938

  Fly  2250 -------------------------QAKCD------------------TQYQKSTSDH------- 2264
                                     ||:.:                  :.|:.|.|||
Mouse   939 EEHHGPPSGTNSARQSPALQHRPMGQAQANHIPGDRIALEGEIGRDVCSSYRHSWSDHKHLAQPD 1003

  Fly  2265 LLLFPARGSQFYQS---------------NFSELPSWNFLLSRWKLTLDLFGRVFMDDVGMEH-G 2313
            ..:....||:..||               .||......:.:..:.|.|...|....|  |..| |
Mouse  1004 TAVISVVGSRHNQSLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKD--GRIHVG 1066

  Fly  2314 SVLPELRGFPVKEMRFRRHMEKLRNGQQRDLVLCKLERNRESLIVQTFKELNTQFGNQSRRI--- 2375
            ..:.|:.|.|.:.:...|.:|.::.|..:.|:|.:....    ::....:.:|...:.|..|
Mouse  1067 DQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTG----LIPDHGDWDTNSPSSSNVIYDE 1127

  Fly  2376 -QPPITFNRVKVTFKDE--PGEGSGVARSFYTSIAEALLASAKIPNLESVQVGTNHSKYVVPFSS 2437
             .||:..:....||::.  |.....:.|......||.|        .::||
Mouse  1128 QPPPLPSSHSASTFEESHVPATEDSLTRVQICEKAEEL--------KDTVQ-------------- 1170

  Fly  2438 ILRSRTVSGSSRDQSTLQRRGSNSKILWRSARERKALNLDARPYTPPNSSDNATPESLNDHLSVH 2502
                       ..:|||  .||..::.::|..:..:..      .||..|.:.....|.....|
Mouse  1171 -----------EKKSTL--NGSQPEMKYQSVHKTMSKK------DPPRGSGHGEKSRLKGENGV- 1215

  Fly  2503 LQQIGERLYPKIHSIN---QTHAPKITGMLLEIPTPQLLSVISSDETLRQKVNEAIEIITFKQKS 2564
             .:.|....|| .|:|   :.|..||...|...|..:     |.|.:|..:..|:...:|.|..|
Mouse  1216 -TRRGRSASPK-KSVNRHSEEHLEKIPRPLKSDPKEK-----SRDRSLSPRKGESKGRLTIKAGS 1273

  Fly  2565 ------ETSAQSSQPKKSPSV 2579
                  :...:||.|||...:
Mouse  1274 GQDPNRKDRGRSSSPKKQQKI 1294

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
hyd	NP_001287262.1	E3_UbLigase_EDD	150..196	CDD:288409
		ZnF_UBR1	1217..1284	CDD:197698
		ASF1_hist_chap	1535..1733	CDD:304562	41/215 (19%)
		PolyA	2498..2561	CDD:197769	16/65 (25%)
		HECTc	2589..2885	CDD:238033
		HECTc	2589..2884	CDD:214523
Magi3	NP_001152826.1	Interaction with ADRB1 and TGFA. /evidence=ECO:0000269\|PubMed:15652357	18..108		4/8 (50%)
		PDZ_signaling	24..103	CDD:238492	2/3 (67%)
		NK	125..>199	CDD:302627	14/96 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	184..266		21/104 (20%)
		WW	297..329	CDD:197736	6/31 (19%)
		WW	345..375	CDD:238122	2/29 (7%)
		Interaction with PTEN. /evidence=ECO:0000250	413..495		20/131 (15%)
		PDZ	416..496	CDD:214570	19/129 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	551..575		8/29 (28%)
		PDZ	578..659	CDD:214570	13/80 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	664..691		6/26 (23%)
		PDZ	729..812	CDD:214570	16/92 (17%)
		Interaction with ADGRB1. /evidence=ECO:0000250	729..811		16/91 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	818..844		4/25 (16%)
		PDZ_signaling	851..936	CDD:238492	17/84 (20%)
		Interaction with LPAR2 and GRIN2B. /evidence=ECO:0000250	852..939		18/86 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..966		1/26 (4%)
		PDZ_signaling	1023..1101	CDD:238492	17/79 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1109..1151		8/41 (20%)
		MIP-T3	<1166..>1431	CDD:287245	35/170 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1168..1476		35/168 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900