DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment hyd and Hecw2

DIOPT Version :9

Sequence 1:	NP_001287262.1	Gene:	hyd / 41181	FlyBaseID:	FBgn0002431	Length:	2887	Species:	Drosophila melanogaster
Sequence 2:	NP_001001883.1	Gene:	Hecw2 / 329152	MGIID:	2685817	Length:	1578	Species:	Mus musculus

Alignment Length:	2000	Identity:	392/2000 - (19%)
Similarity:	669/2000 - (33%)	Gaps:	672/2000 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly  1129 REHALTILQQMCVSPALRPYLC-HMLSTKDAQGQTPFMLSVSCRAYEAGIILLNTILMLSEQDPQ 1192
            |||.|.:.::   :|.:|..|. ..|.:..||...|..:::. ||..      :|.|:.||....
Mouse     6 REHLLFVRRR---NPQMRYTLSPENLQSLAAQNSMPENMALQ-RANS------DTDLVTSESRSS 60

  Fly  1193 LKEAM----------------IFPNGSPADQSPLHVICYNDTCSFTWTGADHINQ----NIFECK 1237
            |..:|                |.....|:|                |.|..||::    |.::.|
Mouse    61 LTASMYEYTLGQAQNLIIFWDIKEEVDPSD----------------WIGLYHIDENSPANFWDSK 109

  Fly  1238 TCGLTGSLCCCTECA-----------------RVCHK---GHDCKLKRTAPTAYCDCWEKC---K 1279
            ..|:||     |:..                 ::|.|   |....|:.|.|         |   |
Mouse   110 NRGVTG-----TQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTP---------CITVK 160

  Fly  1280 CKALI----------AGNLTKRFALLCKLVSCT--DLVTKFNSKG-----ESILLFLIQTVGRQ- 1326
            ..|::          :|:|..|     ||||.|  ||......||     :..|...||. |::
Mouse   161 NPAVMMGAEGMEGGASGSLHSR-----KLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQP-GKKS 219

  Fly  1327 ----IVEQRQYRFSVRVRNVSTAATGATGNNSVISNRKTSAAEIDNDMPDHDLEPPKFA--RKAL 1385
                .....|.|.|..:.|.:         |.:....|.|...:..|:.:.::: .|||  |..:
Mouse   220 SFPTCAHHGQERRSTIISNTT---------NPIWHREKYSFFALLTDVLEIEIK-DKFAKSRPII 274

  Fly  1386 ERLLIDWNAVRSMIMSGAERGDVPNPAGSASENSNSEGFNMFIQTQHGSTLLDKFTHSLIVKCTS 1450
            :|.|                |.:..|.            ...::.|.|..:|   :::|..:..:
Mouse   275 KRFL----------------GKLTIPV------------QRLLERQAGDQML---SYNLGRRLPA 308

  Fly  1451 DHLDTLLLTLVRELQNASVSNRSKEAEEVVRRFVRSVARVFVIFNLEKQPNPEKRK--SH----- 1508
            ||:...|...| |:.:::..:.|.||       |.::..|..:......|:.|:..  ||
Mouse   309 DHVSGYLQFKV-EVTSSAHEDASPEA-------VGTILGVHTVNGDLGSPSDEEDMPGSHHDSTI 365

  Fly  1509 ---------SSCNKYVQSCVKVFQTLHKISIEELCEVSEALIAPVR---------LGVVR---PT 1552
                     |..:...:...:...|| :|..|:|...| :..:|.|         |..:.   |.
Mouse   366 CANGPVSEDSVADGTPKHSFRTSSTL-EIDTEDLISTS-SRNSPPRGRQDSLNDYLDAIEHNGPA 428

  Fly  1553 APFTMSSSNLDVRNSDDLFSVDPL-----APSNVESPSEQILVHDAGNDQSANFNIQQNYDVV-- 1610
            .|...|||...:..|..|.|..|.     |..:::|..|.   |:...|.....::::...::
Mouse   429 RPGAASSSERSMGASPKLRSSFPTDTRLNAMLHIDSDEED---HEFQQDLGYPSSLEEEGGLIMC 490

  Fly  1611 -AMETIRDASESEEVINREANSHNQDDELIENQR---NEDGMQDDESDNDFTFNDAETESDS--D 1669
             ....|.|.|.:.:       :..:||..:||:.   :|....::..:|.....|....|.:  |
Mouse   491 SRASRIDDGSLTSQ-------TKPEDDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPD 548

  Fly  1670 DNQSNQEVQRSVQAGATVGSENDIGVLFLEDESGDSSAQEEDGSEDGESDDQ--SDEFNFNEQQL 1732
            :|::|.|.|.|...|:|            |..|.....|...|::.|.||..  |.......:.|
Mouse   549 ENEANLEPQPSADQGST------------ELCSSQEVDQPTSGADAGASDTSGGSRRAASETESL 601

  Fly  1733 ERRSTNSNARSDLAPQTMQWAIRSRDTARSSVRVPTGSNMVFIDPMALRRSTVPASTTVTTPSIE 1797
            ::.|..|...|:..|..   ..|:...:.:|.| |.|.:    ||.. ..|:...|.|....|:|
Mouse   602 DQGSEPSQVSSETEPSD---PARTESVSEASTR-PEGES----DPEG-ADSSCNESVTTQLSSVE 657

  Fly  1798 PHTMATTASNLARAFGITIRQISELISILSYNVLNDIETSLKIQNDEAIAVQAFVEKRLKATWDW 1862
                 |..|:|..|      :..|..:..|             |.:|
Mouse   658 -----TRCSSLESA------RFPETPAFSS-------------QEEE------------------ 680

  Fly  1863 MFTVMDGTEAQLKFGAYLTNYTDPNHPLHPLNLSAQASSSQTPAPATSSSVNGVNIMGSNSRRDF 1927
                 ||.                         .|...:|..||..:..||
Mouse   681 -----DGA-------------------------CAAEPTSSGPAEGSQESV-------------- 701

  Fly  1928 FTYCLSLMRSHTSEHRDALPVLDITALRHIAYVLDAFVYYMRNDSG-FYDKQDTISGRINNLSPM 1991
               |..          .:||.:.:.:........:|.....:.:.| .:.::.::.|.. ..:|.
Mouse   702 ---CTP----------SSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAA-AAAPA 752

  Fly  1992 TESYDTDDELANLEEFNA-DVQMSASSMPSGSQGTRRHAFFARSESTLSLGCSAPEGFELPLDMA 2055
            ..:.|:..:    |:.:| |.|.:.....:..:|              :.|.|...|.:
Mouse   753 AAATDSQPQ----EDGDAGDAQGACEGATAQEEG--------------ATGGSQTNGHQ------ 793

  Fly  2056 MPLADKPHLLQPNSKRQELFANLPLLVTTNANNSGATNDGDGGSIF-DY--------------TP 2105
             ||...|.:.|..|:.|.:...||       .|..|..|..|...: |:              .|
Mouse   794 -PLRSLPSVRQDVSRYQRVDEALP-------PNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAP 850

  Fly  2106 TRLGFSNSLKRNE---RVYETVPIDSSKTGDGNVTNKAEGSTDSNIYVQLKKKQGSDDFKSHKEA 2167
            ..|..|||:::.|   |.|:::    .:|    :||:......|.|       .|:.:     ||
Mouse   851 QVLQRSNSIQQMEQLNRRYQSI----RRT----MTNERPEENTSAI-------DGAGE-----EA 895

  Fly  2168 DGNQSKYEKVVLMETDDSLP-STSKSTEALMATRPEV--IIAPNKASV--SPATAARSVIVLAGG 2227
            |.:|:..:    ...::.|| |||:|...|:...|.|  :|:|...:|  |..:|.|   :....
Mouse   896 DFHQASAD----FRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYR---MFTNN 953

  Fly  2228 SCLKTIDSDINNYSASNLSTAEQAKCDTQYQKSTSDHLLLFPARGSQFYQSNFSELPSWNFLLSR 2292
            :|||.:.:.:..              ||.:               .:.||.|...:...|...::
Mouse   954 TCLKHMITKVRR--------------DTHH---------------FERYQHNRDLVGFLNMFANK 989

  Fly  2293 -------WKLTLDLFGRVFMDDVGMEHGS--------VLPELRGFPVKEMRFRRHMEKLRNGQQR 2342
                   |::..|..|:.|.    ::|.|        .||.....|...:..|:|:.:.|:....
Mouse   990 QLELPRGWEMKHDHQGKAFF----VDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAG 1050

  Fly  2343 DLVLCKLERNRESLIVQTFKELNTQFGNQSRRIQPPI------TFNRVKVTFKDEPGEGSGVARS 2401
            ::                        |..||...||:      |||.              |:|.
Mouse  1051 EV------------------------GEDSRHAGPPVLPRPSSTFNT--------------VSRP 1077

  Fly  2402 FYTSIA-----EALLASAKIPN-LESVQ-----VGTNHSKYVVPFSSILRSRTVSGSSRDQSTLQ 2455
            .|..:.     :.::|..:.|| ||.:|     :..|||         ||.:.....:.....|.
Mouse  1078 QYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHS---------LREKIQFIRTEGTPGLV 1133

  Fly  2456 RRGSNSK-ILWRSARERKALNLDARPYTPPNS--------SDNATPESLNDHLSVHLQQIGERLY 2511
            |..|::. ::..|..|.:.::     |.||::        |...:|.|
Mouse  1134 RLSSDADLVMLLSLFEEEIMS-----YVPPHALLHPSYCQSPRGSPVS----------------- 1176

  Fly  2512 PKIHSINQTHAPKITGMLLEIPTPQLLSVISSDETLRQKVNEAIEIITFKQKSETSAQSSQPKKS 2576
                  :..::|.........|.|             .|.:...::..|.:|.||......|.|.
Mouse  1177 ------SPQNSPGTQRANARAPAP-------------YKRDFEAKLRNFYRKLETKGYGQGPGKL 1222

  Fly  2577 PSVVVVDPVDDD------------------------NEPLFYSPGKRGF--------YTPRQGFA 2609
            ..::..|.:.:|                        .|.|.||...|.|        :.|..|..
Mouse  1223 KLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLF 1287

  Fly  2610 SFERINA------------------FRNIGRLIGLCLLQNELLPLFLQRHVLKYILGRKIKFHDL 2656
            .:...:.                  ||..||::||.|:...||..|..|...|.:|.......||
Mouse  1288 EYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDL 1352

  Fly  2657 AFFDPALYESFRQIIQNAQTKEGEETINRMELCFVIDLMKEEGCG---NRELIPGGRDVAVTSSN 2718
            .:.|    |.|.|.:|..:..:..:.   ::|.|.::   ||..|   .|||.|||.::.||..|
Mouse  1353 EYLD----EEFHQSLQWMKDNDIHDI---LDLTFTVN---EEVFGQITERELKPGGANIPVTEKN 1407

  Fly  2719 IFEYVRRYTEYRLIKSQEKALEALKDGVFDVLPDNSMINLTAEDLRLLLNGVGDINVSTLISYTT 2783
            ..||:.|..::|:.:...:..|:|..|.::|:....:....|.:|.|::.|..:|:::...:.|.
Mouse  1408 KKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTE 1472

  Fly  2784 FNDESSEGPDKLLKFKKWFWSIVEKMNIMERQDLVYFWTGSPALPASEEGFQPLPSVTIRPADD- 2847
            :.....:....:    :|||:.||:.|..:|..|:.|.||:.::|  .|||     .::|.::.
Mouse  1473 YRGGYHDNHIVI----RWFWAAVERFNNEQRLRLLQFVTGTSSIP--YEGF-----ASLRGSNGP 1526

  Fly  2848 -----------SHLPTANTCISRLYIPLYSSKSILRSKMLMAI-KSKNFG 2885
                       :.||.|:||.:||.:|.|.|.|:|..|:|.|: ::..||
Mouse  1527 RRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFG 1576

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
hyd	NP_001287262.1	E3_UbLigase_EDD	150..196	CDD:288409
		ZnF_UBR1	1217..1284	CDD:197698	19/93 (20%)
		ASF1_hist_chap	1535..1733	CDD:304562	45/224 (20%)
		PolyA	2498..2561	CDD:197769	4/62 (6%)
		HECTc	2589..2885	CDD:238033	87/337 (26%)
		HECTc	2589..2884	CDD:214523	87/336 (26%)
Hecw2	NP_001001883.1	HECW_N	45..164	CDD:374632	29/154 (19%)
		C2_NEDL1-like	185..320	CDD:176073	35/177 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	341..453		23/113 (20%)
		DMP1	<344..700	CDD:369294	86/460 (19%)
		NESP55	<463..639	CDD:115071	42/205 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	496..802		79/458 (17%)
		Interaction with TP73. /evidence=ECO:0000250	737..1074		86/467 (18%)
		WW	815..844	CDD:366073	7/35 (20%)
		HECW1_helix	921..987	CDD:375862	18/97 (19%)
		WW	993..1022	CDD:366073	6/32 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1030..1075		12/82 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1167..1193		4/48 (8%)
		HECTc	1222..1576	CDD:238033	89/374 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900