Protein Alignment CG16789 and Spast

DIOPT Version :10

• Sequence 1: NP_649910.3
• Gene: CG16789 / 41154
• FlyBaseID: FBgn0037712
• Length: 831
• Species: Drosophila melanogaster
• Sequence 2: NP_001156342.1
• Gene: Spast / 50850
• MGIID: 1858896
• Length: 614
• Species: Mus musculus
• Alignment Length: 591
• Identity: 124/591 - (20%)
• Similarity: 227/591 - (38%)
• Gaps: 147/591 - (24%)

  Fly   260 PPPPAVVKERAEKLEKLQHNEKRYQAQAFYGKEFEERQNALKEEIRAKHGASMKEDIADEIRAWV 324
            ||.|....| ||.:            :.|:.:.||....||:  |..:..|..||...:    |.
Mouse   102 PPEPGPGGE-AESV------------RVFHKQAFEYISIALR--IDEEEKAGQKEQAVE----WY 147

  Fly   325 KDCYDKTGKLPDFPSEDQGGSLHIFSRPGTESEHSRS-SARSSKESRKTKDKSKSPARSGDLNVN 388
            |...::..|          |...|.:..|.:.|.:|. .|:........||:         |.:.
Mouse   148 KKGIEELEK----------GIAVIVTGQGEQYERARRLQAKMMTNLVMAKDR---------LQLL 193

  Fly   389 ENQDEEVSFQPAPSVCLPDIRAEIDLFND----TWRD---KDETGVLSQAAYEDMIYSDKYQEVE 446
            |.....:.|.          :::.|::|:    |.|:   :.|:|.:.:.  :|.:
Mouse   194 EKLQPVLQFS----------KSQTDVYNESTNLTCRNGHLQSESGAVPKR--KDPL--------- 237

  Fly   447 LEFRRAVDDVMRQEIELLQTAVG--------------------------KKVKKSSKKTRRSGKK 485
               ..|.:.:.|.:..|...:.|                          ....|.:.|..|:.|.
Mouse   238 ---THASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPKPNRTNKP 299

  Fly   486 SKK----KKEKDLTPDRTTES-----LYEELVTNGIIRKYPELRLKQFLGDKALTARIGTNPSPG 541
            |..    :|:|||...|..:|     :..|:|.||...|:.::..::.
Mouse   300 STPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQEL----------------- 347

  Fly   542 DIRQILTEYCILP-LGSDAIHNCTPLIRSILLAGPKGSGKKALLHAICTEVGAVLFDLTPANIVG 605
             .:|.|.|..||| |..:.........|.:||.||.|:||..|..|:..|..|..|:::.|::..
Mouse   348 -AKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTS 411

  Fly   606 KYPGKSGLIMLIHLVLKVSRLLQPAVIFMGDAERPFMKKIPKTDRTDPKRLKKDLPKLIK----N 666
            ||.|:..  .|:..:..|:|.|||::||:.:.:....:: .:.:....:|||.:.  ||:    .
Mouse   412 KYVGEGE--KLVRALFAVARELQPSIIFIDEVDSLLCER-REGEHDASRRLKTEF--LIEFDGVQ 471

  Fly   667 IAPEDRVVFIGTSNLPWEADQKLLQSVYNRFIYIPRPDYGAMSHAWKTLLHDYSGGISNLDTSAM 731
            .|.:|||:.:|.:|.|.|.|:.:|:....| :|:..|:........|.||......::..:.:.:
Mouse   472 SAGDDRVLVMGATNRPQELDEAVLRRFIKR-VYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQL 535

  Fly   732 AKISDGYTIGSIDACLKEVMTCKRKLQLRTQPLTN---AELINVLCS---------RDPVYREEE 784
            |:::|||:...:.|..|:......: :|:.:.:.|   :|:.|:..|         :..|..:..
Mouse   536 ARMTDGYSGSDLTALAKDAALGPIR-ELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTL 599

  Fly   785 EAFESW 790
            ||:..|
Mouse   600 EAYIRW 605

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG16789	NP_649910.3	DUF5401	<131..>307	CDD:375164	12/46 (26%)
		SpoVK	443..773	CDD:440232	83/372 (22%)
		P-loop containing Nucleoside Triphosphate Hydrolases	542..700	CDD:476819	48/162 (30%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..298		43/257 (17%)
		Required for midbody localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..192		27/127 (21%)
		Required for interaction with ATL1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	91..112		4/10 (40%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250|UniProtKB:Q9UBP0	110..194		24/121 (20%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	112..198		23/122 (19%)
		MIT_spastin	114..193	CDD:239142	21/115 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	220..306		12/99 (12%)
		Sufficient for microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	226..614		91/419 (22%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	268..326		11/57 (19%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	307..310		1/2 (50%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	308..310		1/1 (100%)
		RecA-like_spastin	341..504	CDD:410932	48/186 (26%)
		AAA_lid_3	531..585	CDD:465537	11/54 (20%)
		Vps4_C	<578..610	CDD:462762	5/28 (18%)